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951.
Pack ice around Svalbard was sampled during the expedition ARK XIX/1 of RV “Polarstern” (March–April 2003) in order to determine
environmental conditions, species composition and abundances of sea-ice algae and heterotrophic protists during late winter.
As compared to other seasons, species diversity of algae (total 40 taxa) was not low, but abundances (5,000–448,000 cells l−1) were lower by one to two orders of magnitude. Layers of high algal abundances were observed both at the bottom and in the
ice interior. Inorganic nutrient concentrations (NO2, NO3, PO4, Si(OH)4) within the ice were mostly higher than during other seasons, and enriched compared to seawater by enrichment indices of
1.6–24.6 (corrected for losses through the desalination process). Thus, the survival of algae in Arctic pack ice was not limited
by nutrients at the beginning of the productive season. Based on less-detailed physical data, light was considered as the
most probable factor controlling the onset of the spring ice-algal bloom in the lower part of the ice, while low temperatures
and salinities inhibit algal growth in the upper part of the ice at the end of the winter. Incorporation of ice algae probably
took place during the entire freezing period. Possible overwintering strategies during the dark period, such as facultative
heterotrophy, energy reserves, and resting spores are discussed. 相似文献
952.
Fröhlich J Koustiane C Kämpfer P Rosselló-Mora R Valens M Berchtold M Kuhnigk T Hertel H Maheshwari DK König H 《Systematic and applied microbiology》2007,30(1):68-74
Wood-eating termites feed on a diet highly deficient in nitrogen. They must complement their diet with the aid of nitrogen-fixing bacteria. Nitrogen fixation in the gut has been demonstrated, but information about nitrogen-fixing bacteria in pure culture is scarce. From the higher termite Nasutitermes nigriceps the symbiotic bacterial strain M3A was isolated, which thrives in the hindgut contents. The Gram-negative strain exhibited similarities to the species of the genus Ensifer (including Sinorhizobium) on the basis of morphological and physiological/biochemical features. The 16S rRNA gene analysis showed the highest sequence similarity of the isolate M3A to Ensifer adhaerens (>99%; ATCC 33499). The DNA-DNA hybridization revealed a similarity of 66% with E. adhaerens (NCIMB12342(T)). In contrast to the type strain the isolate M3A possesses the capacity to nodulate plant roots. This is the first report on the detailed identification of a rhizobia-related strain from the intestinal tract of animals. Strain M3A has been deposited with two culture collections (DSM10169; ATCC BAA-396). 相似文献
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Due to the species’ deep burrowing behaviour, growth parameters of the soft-shell clam Mya arenaria L. 1758 are difficult to estimate, especially in deeper habitats which are not directly accessible. In this study, we analysed
192 specimens of M. arenaria and found, contrary to results of most other studies that the sigmoid Gompertz growth model (GGM) is appropriate to describe
the growth. Predictions from this model confirm the finding of a life span up to 8 years and as a consequence of this, a maximum
shell-length of 60 mm. Individual growth rates calculated from the first deviation indicate a nearly exponential growth in
young individuals. The relationship between the clam sipho-width and age, shell-length and biomass conform to the GGM. This
is a new approach to assess growth of M. arenaria and to solve practical problems that arise in field studies. 相似文献
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Pseudoporphyria (PP) is characterized by skin fragility, blistering and scarring in sun-exposed skin areas without abnormalities
in porphyrin metabolism. The phenylpropionic acid derivative group of nonsteroidal anti-inflammatory drugs, especially naproxen,
is known to cause PP. Naproxen is currently one of the most prescribed drugs in the therapy of juvenile idiopathic arthritis
(JIA). The prevalence of PP was determined in a 9-year retrospective study of children with JIA and associated diseases. In
addition, we prospectively studied the incidence of PP in 196 patients (127 girls and 69 boys) with JIA and associated diseases
treated with naproxen from July 2001 to March 2002. We compared these data with those from a matched control group with JIA
and associated diseases not treated with naproxen in order to identify risk factors for development of PP. The incidence of
PP in the group of children taking naproxen was 11.4%. PP was particularly frequent in children with the early-onset pauciarticular
subtype of JIA (mean age 4.5 years). PP was associated with signs of disease activity, such as reduced haemoglobin (<11.75
g/dl), and increased leucocyte counts (>10,400/μl) and erythocyte sedimentation rate (>26 mm/hour). Comedications, especially
chloroquine intake, appeared to be additional risk factors. The mean duration of naproxen therapy before the onset of PP was
18.1 months, and most children with PP developed their lesions within the first 2 years of naproxen treatment. JIA disease
activity seems to be a confounding factor for PP. In particular, patients with early-onset pauciarticular JIA patients who
have significant inflammation appear to be prone to developing PP upon treatment with naproxen. 相似文献
960.
Integral and associated lysosomal membrane proteins 总被引:2,自引:0,他引:2
Schröder B Wrocklage C Pan C Jäger R Kösters B Schäfer H Elsässer HP Mann M Hasilik A 《Traffic (Copenhagen, Denmark)》2007,8(12):1676-1686
We searched for novel proteins in lysosomal membranes, tentatively participating in molecular transport across the membrane and/or in interactions with other compartments. In membranes purified from placental lysosomes, we identified 58 proteins, known to reside at least partially in the lysosomal membrane. These included 17 polypeptides comprising or associated with the vacuolar adenosine triphosphatase. We report on additional 86 proteins that were significantly enriched in the lysosomal membrane fraction. Among these, 12 novel proteins of unknown functions were found. Three were orthologues of rat proteins that have been identified in tritosomes by Bagshaw RD et al. (A proteomic analysis of lysosomal integral membrane proteins reveals the diverse composition of the organelle. Mol Cell Proteomics 2005;4:133-143). Here, the proteins encoded by LOC201931 (FLJ38482) and LOC51622 (C7orf28A) were expressed with an appended fluorescent tag in HeLa cells and found to be present in lysosomal organelles. Among the lysosomally enriched proteins, also 16 enzymes and transporters were detected that had not been assigned to lysosomal membranes previously. Finally, our results identified a particular set of proteins with known functions in signaling and targeting to be at least partially associated with lysosomes. 相似文献