全文获取类型
收费全文 | 3587篇 |
免费 | 248篇 |
出版年
2021年 | 24篇 |
2020年 | 25篇 |
2019年 | 26篇 |
2018年 | 47篇 |
2017年 | 38篇 |
2016年 | 60篇 |
2015年 | 103篇 |
2014年 | 133篇 |
2013年 | 180篇 |
2012年 | 178篇 |
2011年 | 181篇 |
2010年 | 117篇 |
2009年 | 88篇 |
2008年 | 157篇 |
2007年 | 210篇 |
2006年 | 192篇 |
2005年 | 172篇 |
2004年 | 172篇 |
2003年 | 156篇 |
2002年 | 149篇 |
2001年 | 48篇 |
2000年 | 35篇 |
1999年 | 57篇 |
1998年 | 39篇 |
1997年 | 43篇 |
1996年 | 37篇 |
1995年 | 41篇 |
1994年 | 36篇 |
1993年 | 45篇 |
1992年 | 29篇 |
1991年 | 29篇 |
1990年 | 36篇 |
1989年 | 39篇 |
1988年 | 46篇 |
1987年 | 46篇 |
1986年 | 25篇 |
1985年 | 42篇 |
1984年 | 31篇 |
1983年 | 45篇 |
1982年 | 43篇 |
1981年 | 38篇 |
1980年 | 40篇 |
1979年 | 32篇 |
1978年 | 35篇 |
1977年 | 33篇 |
1976年 | 24篇 |
1975年 | 29篇 |
1974年 | 25篇 |
1973年 | 28篇 |
1970年 | 27篇 |
排序方式: 共有3835条查询结果,搜索用时 15 毫秒
41.
Helmut Leonhardt 《Cell and tissue research》1967,84(1):1-8
Zusammenfassung In der Gegend der Apertura lateralis ventriculi quarti des Kaninchengehirns wurden intraventrikuläre markhaltige Nervenfasern der Fasergruppe B gefunden. Ihre Markscheide beginnt oder endigt innerhalb des Ventrikellumens. Besonderheiten des Ependyms dieser Gegend werden mitgeteilt.
Die Untersuchung wurde mit dankenswerter Unterstützung durch die Deutsche Forschungsgemeinschaft durchgeführt. 相似文献
Summary In the 4th ventricle of the brain near the apertura lateralis in rabbit myelinated nerve fibers occur. These nerve fibers belong to the fiber group B. Their myelin sheath begins or ends in the ventricular cavity. Peculiarities of the ependyma of this region are discussed.
Die Untersuchung wurde mit dankenswerter Unterstützung durch die Deutsche Forschungsgemeinschaft durchgeführt. 相似文献
42.
Helmut Leonhardt 《Cell and tissue research》1967,76(4):511-524
Zusammenfassung Die sinusförmigen Kapillaren der Area postrema werden von zwei Basalmembranen umgeben, die einen Raum einschließen, in dem Makrophagen liegen. Verästelte Ausläufer der äußeren Basalmembran dringen tief zwischen die Gliafortsätze ein und bilden ein Membranlabyrinth. Das Kapillarendothel ist fenestriert. Nach Cardiazolkrampf entsteht ein perivaskuläres Oedem, das das Membranlabyrinth öffnet und den perivaskulären Raum vergrößert. Das Endothel zeigt hierbei starke Cytopempsis. Die funktionelle Bedeutung der Strukturen wird diskutiert.
Mit dankenswerter Unterstützung durch die Deutsche Forschungsgemeinschaft. 相似文献
Summary The sinusoid capillaries of the area postrema are surrounded by two basement membranes enclosing a perivascular space, in which macrophages are found. Ramified extensions of the outer basement membrane penetrate deeply between the glial processes forming a labyrinth of basement membranes. The capillary endothelium is fenestrated. After a Cardiazolinduced convulsion a perivascular edema opens the basement membrane labyrinth and the perivascular space is greatly enlarged. The endothelium shows pinocytotic activity. The functional significance of these structures is discussed.
Mit dankenswerter Unterstützung durch die Deutsche Forschungsgemeinschaft. 相似文献
43.
Helmut Leonhardt 《Cell and tissue research》1967,79(2):172-184
Zusammenfassung In der vorderen Wand des IV. Ventrikels und des Canalis centralis gegenüber der Area postrema liegen beim Kaninchen Nervenzellen, deren Fortsätze intraventrikulär mit 6 großen Protoplasmakugeln endigen, die mit Mitochondrien angefüllt sind. Das Perikaryon ist reich an Ergastoplasma, alle Teile der Zelle enthalten leere, 600 bis 1500 Å messende, und ein dichtes Granulum enthaltende, 650–1000 Å große Bläschen. Vermutlich werden Stoffe in den Liquor cerebrospinalis abgegeben. Dem Plasmalemm der Nervenzellen liegen Synapsen an.
Die Untersuchung wurde mit dankenswerter Unterstützung durch die Deutsche Forschungsgemeinschaft durchgeführt. 相似文献
Summary In the anterior wall of the IVth ventricle, opposite to the area postrema, nerve cells are found. Their processes end with spheroid swellings extending into the ventricle. These bulbs contain a large number of mitochondria, while ergastoplasm is seen in the perikaryon. The cytoplasm of the whole cell shows vesicles (600–1500 Å) and dense-core vesicles (650–1000 Å) which might be extruded into the liquor cerebrospinalis. The plasmalemma of the perikaryon is in contact with synapses.
Die Untersuchung wurde mit dankenswerter Unterstützung durch die Deutsche Forschungsgemeinschaft durchgeführt. 相似文献
44.
The antioxidant capacity of 5-hydroxy-tryptophan. 5-hydroxy-indole. and DOPA (3,4-dihydroxy-phenyI-alanine) was tested in the Fe-induced lipid peroxidation of liver microsomes of normal- and vitamin E-deficient rats, using ascorbate as a reductant. Lipid peroxidation was monitored as low-level chemilu-minescence, indicative of generation of electronically-excited states arising from the recombination of secondary lipid peroxyl radicals. 相似文献
45.
A. Moser B. Mayr W. Jury W. Steiner P. Horvat 《Bioprocess and biosystems engineering》1991,7(4):177-182
The macroscopic mathematical model based on compartments with ideal mixing zones and tanks-in series was evaluated. Based on the experimental data obtained in a 300 dm3 pilot reactor and the dependence of mixing time on the volume of liquid phase, we have found mathematical relations between the ratio of vessel diameter to liquid level, adjustable parameters of model and the mixing time.List of Symbols
V dm3
total volume of bioreactor
-
V
g dm3
total volume of liquid
-
V
1 dm3
volume of ideally mixed zone in the vessel
-
V
2 dm3
volume of macromixer in inner circulation flows
-
V
3 dm3
volume of liquid phase in the pump
-
V
4 dm3
volume of liquid phase in the pipe between the vessel and the pump
-
V
5 dm3
volume of liquid phase in the pipe between the pump and air input system included falling jet
-
V
LT dm3
volume of liquid in the tank
-
V
LC dm3
volume of liquid in the circulation system
-
F
E dm3/s
inner volumetric circulation flow rate across the macromixers
-
F
cir dm3/s
external volumetric circulation flow rate, pumping capacity
-
t
A s
time interval of the pulse application
-
t
AA s
time point of the pulse application related to the free choosen starting point of the experiment
-
t
m s
mixing time
-
t
c s
circulation time
-
t
end s
end time of simulation
-
C
*,* kg/m3
concentration of tracer in the indicated compartment
-
C
0 kg/m3
concentration of the tracer before the injection
-
C
t kg/m3
concentration of the tracer at the indicated time
-
C
kg/m3
theoretical concentration of the full mixed tracer
-
C
sim kg/m3
calculated concentration of tracer during numerical integration method
-
i
index of an arbitrary tank
-
D
T m
diameter of bioreactor
-
D 1/s
dilution rate
-
H
L m
level of liquid in the unaerated vessel
-
vector of inhomogenities 相似文献
46.
The reaction of ribose with horseradish peroxidase in the presence of H2O2 is accompanied by light emission. The detection of horseradish peroxidase Compound II (FeO2+) indicates that the enzyme participates in a normal peroxidatic cycle. Hydrogen peroxide converts horseradish peroxidase into Compound I (FeO3+) which in turn is converted into Compound II by abstracting a hydrogen atom from ribose forming a ribosyl radical. In aerated solutions oxygen rapidly adds to the ribosyl radical. Based on the spectral characteristics and the enhancement of the chemiluminescence by chlorophyll-a, xanthene dyes, D2O and DABCO, it is suggested that the excited species, apparently triplet carbonyls and 1O2, are formed from the bimolecular decay of the peroxyl radicals via the Russell mechanism. 相似文献
47.
Crystals of the NC1 domain of human type IV collagen 总被引:1,自引:0,他引:1
M Stubbs L Summers I Mayr M Schneider W Bode R Huber A Ries K Kühn 《Journal of molecular biology》1990,211(4):683-684
Crystals of the non-collagenous C-terminal region (NC1) of type IV collagen have been obtained from human placenta. These crystals diffract to 2.0 A, and belong to space group P22(1)2(1), with cell dimensions a = 81 A, b = 158 A, c = 138 A, alpha = beta = gamma = 90 degrees. The crystals contain one hexamer in the asymmetric unit; they are very stable with respect to X-rays. 相似文献
48.
Clinical and biochemical studies in three patients with severe early infantile Cockayne syndrome 总被引:2,自引:0,他引:2
Jaak Jacken Helmut Klocker Helga Schwaiger Romuald Bellmann Monica Hirsch-Kauffmann Manfred Schweiger 《Human genetics》1989,83(4):339-346
Summary We present clinical and biochemical data from three patients with severe Cockayne syndrome (CS) of very early onset. Unlike in classic CS, signs became evident in the first weeks of life and led to unusually early death. Fibroblasts from two of the patients showed a complete defect of the repair of UV-induced thymine dimer lesions. They were unable to remove thymine dimer lesions from their DNA, had a severe reduction of the RNA synthesis rates after UV irradiation, and showed no reactivation of an UV-inactivated indicator gene and no DNA recondensation after UV irradiation. DNA repair investigated in these two fibroblast cell strains resembled that of xeroderma pigmentosum cells of complementation group A. In contrast, fibroblasts from the third patient showed the same in vitro repair characteristics as classic CS cells. 相似文献
49.
Helmut Wieczorek Gabriele Wolff 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1989,164(6):825-834
1. | The electrophysiology of the sugar receptor in labellar taste hairs ofDrosophila melanogaster (Diptera) was investigated using 33 monosaccharides, oligosaccharides, glucosides, and amino acids which in calyptrate flies are known to bind to specific receptor sites or which may be assigned to specific receptor sites on the basis of structural criteria. |
2. | The pyranose site ofDrosophila is very similar to the pyranose site of the calyptrate flies: regarding monosaccharides of the pyranose type three adjacent equatorial hydroxyl groups (C-2, C-3, C-4) seem to be important for stimulating effectiveness. On the other hand, it exhibits a more rigid stereospecificity with regard to the substituents at C-1 and C-5. |
3. | A furanose site as in calyptrate flies does not exist inDrosophila. First, D-galactose, phenylalanine and 2,5-anhydro-D-mannitol are not or nearly not stimulatory. Secondly, according to different sugar receptor responses after treatment of the taste hairs with papain, D-fucose binds to another receptor site than D-fructose. Thirdly, the effective conformation of D-fructose is not the furanose, but most probably the pyranose form as can be concluded from experiments with freshly prepared and equilibrium solutions of D-fructose. |
4. | The characteristic differences between the properties of the sugar receptors ofDrosophila and of the calyptrate flies lead to the suggestion that the actual number of types of receptor sites in the various fly species is greater than assumed up till now. The broad specificity of the sugar receptors of flies may therefore result from a mosaic of different types of highly specific receptor sites. |
50.
Identification and purification of a family of dimeric major cold shock protein homologs from the psychrotrophic Bacillus cereus WSBC 10201. 总被引:2,自引:1,他引:1
下载免费PDF全文
![点击此处可从《Journal of bacteriology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Whole-cell protein patterns of a psychrotrophic Bacillus cereus strain from cultures grown at 7 and 30 degrees C were compared. This analysis revealed that at least three major proteins are expressed at a significantly higher rate at 7 degrees C than at 30 degrees C. The most abundant of these cold-induced proteins was a small polypeptide of 7.5 kDa, designated CspA, of B. cereus. In addition, four small proteins very similar in size to CspA were seen on both 7 degrees C and 30 degrees C two-dimensional protein gels. Immunoblot analysis using B. cereus anti-CspA antibodies indicated that the five proteins described above plus an additional sixth protein not visible on silver-stained two-dimensional gels are members of a B. cereus cold shock protein family. This hypothesis was corroborated by cloning and sequencing of the genes encoding five proteins of this family. The protein sequences deduced are highly similar and show homology to small procaryotic cold shock proteins and to the cold shock domain of eucaryotic Y-box proteins. Besides CspA, only one of the additional five CspA homologs was slightly cold inducible. In the presence of 100 mM NaCl, the two purified members of the protein family (CspA and CspE) elute as dimers at an apparent molecular mass of 15 kDa from a gel filtration column. At higher salt concentrations, they dissociate into their monomers. Their ability to bind to the ATTGG motif of single-stranded oligonucleotides was demonstrated by band shift analysis. 相似文献