全文获取类型
收费全文 | 3034篇 |
免费 | 197篇 |
出版年
2020年 | 19篇 |
2018年 | 32篇 |
2017年 | 29篇 |
2016年 | 45篇 |
2015年 | 83篇 |
2014年 | 109篇 |
2013年 | 156篇 |
2012年 | 150篇 |
2011年 | 158篇 |
2010年 | 93篇 |
2009年 | 82篇 |
2008年 | 140篇 |
2007年 | 186篇 |
2006年 | 173篇 |
2005年 | 156篇 |
2004年 | 154篇 |
2003年 | 138篇 |
2002年 | 131篇 |
2001年 | 28篇 |
2000年 | 21篇 |
1999年 | 35篇 |
1998年 | 33篇 |
1997年 | 34篇 |
1996年 | 31篇 |
1995年 | 37篇 |
1994年 | 27篇 |
1993年 | 41篇 |
1992年 | 19篇 |
1991年 | 21篇 |
1990年 | 27篇 |
1989年 | 29篇 |
1988年 | 36篇 |
1987年 | 30篇 |
1985年 | 31篇 |
1984年 | 27篇 |
1983年 | 40篇 |
1982年 | 37篇 |
1981年 | 36篇 |
1980年 | 40篇 |
1979年 | 30篇 |
1978年 | 33篇 |
1977年 | 28篇 |
1976年 | 23篇 |
1975年 | 24篇 |
1973年 | 25篇 |
1972年 | 18篇 |
1971年 | 18篇 |
1970年 | 21篇 |
1967年 | 18篇 |
1960年 | 20篇 |
排序方式: 共有3231条查询结果,搜索用时 921 毫秒
131.
Katarina Stingl Karl Ulrich Bartz-Schmidt Dorothea Besch Angelika Braun Anna Bruckmann Florian Gekeler Udo Greppmaier Stephanie Hipp Gernot H?rtd?rfer Christoph Kernstock Assen Koitschev Akos Kusnyerik Helmut Sachs Andreas Schatz Krunoslav T. Stingl Tobias Peters Barbara Wilhelm Eberhart Zrenner 《Proceedings. Biological sciences / The Royal Society》2013,280(1757)
This study aims at substituting the essential functions of photoreceptors in patients who are blind owing to untreatable forms of hereditary retinal degenerations. A microelectronic neuroprosthetic device, powered via transdermal inductive transmission, carrying 1500 independent microphotodiode-amplifier-electrode elements on a 9 mm2 chip, was subretinally implanted in nine blind patients. Light perception (8/9), light localization (7/9), motion detection (5/9, angular speed up to 35 deg s−1), grating acuity measurement (6/9, up to 3.3 cycles per degree) and visual acuity measurement with Landolt C-rings (2/9) up to Snellen visual acuity of 20/546 (corresponding to decimal 0.037 or corresponding to 1.43 logMAR (minimum angle of resolution)) were restored via the subretinal implant. Additionally, the identification, localization and discrimination of objects improved significantly (n = 8; p < 0.05 for each subtest) in repeated tests over a nine-month period. Three subjects were able to read letters spontaneously and one subject was able to read letters after training in an alternative-force choice test. Five subjects reported implant-mediated visual perceptions in daily life within a field of 15° of visual angle. Control tests were performed each time with the implant''s power source switched off. These data show that subretinal implants can restore visual functions that are useful for daily life. 相似文献
132.
133.
Thomas Weisse Michael Moser Ulrike Scheffel Peter Stadler Thomas Berendonk Guntram Weithoff Helmut Berger 《European journal of protistology》2013,49(2):255-271
We investigated the morphology, phylogeny of the 18S rDNA, and pH response of Oxytricha acidotolerans sp. nov. and Urosomoida sp. (Ciliophora, Hypotricha) isolated from two chemically similar acid mining lakes (pH ~ 2.6) located at Langau, Austria, and in Lusatia, Germany. Oxytricha acidotolerans sp. nov. from Langau has 18 frontal-ventral-transverse cirri but a very indistinct kinety 3 fragmentation so that the assignment to Oxytricha is uncertain. The somewhat smaller species from Lusatia has a highly variable cirral pattern and the dorsal kineties arranged in the Urosomoida pattern and is, therefore, preliminary designated as Urosomoida sp. The pH response was measured as ciliate growth rates in laboratory experiments at pH ranging from 2.5 to 7.0. Our hypothesis was that the shape of the pH reaction norm would not differ between these closely related (3% difference in their SSU rDNA) species. Results revealed a broad pH niche for O. acidotolerans, with growth rates peaking at moderately acidic conditions (pH 5.2). Cyst formation was positively and linearly related to pH. Urosomoida sp. was more sensitive to pH and did not survive at circumneutral pH. Accordingly, we reject our hypothesis that similar habitats would harbour ciliate species with virtually identical pH reaction norm. 相似文献
134.
Robert Gliniars Gabriel Sebastian Becker Désirée Braun Helmut Dalitz 《Trees - Structure and Function》2013,27(4):1129-1138
Monthly stem increment of 766 trees was assessed for 7 years in Kakamega Forest, Kenya and related to monthly climatic variables. Mean stem increment of all tree individuals correlated negatively with maximum temperature but not with mean and minimum temperatures. For the precipitation variables sum of precipitation and number of rainy days we found positive correlations. Stem increment of the trees in the early-, mid-, and late-successional groups correlated positively with the number of rainy days. For late-successional trees increment correlated negatively with mean and maximum temperature and positively with all other precipitation variables. For mid-successional trees we found a negative correlation with mean temperature. In addition, the stem increment of most species related positively to precipitation variables and negatively to mean and maximum temperature. In view of the expected increasing temperatures and fewer but heavier rain events, our results suggest that climate change will lead to a reduction in stem increment. The results appertaining to the successional groups imply that early and mid-successional species are better equipped to perform well under the expected future climatic conditions than the late-successional species. This could reduce the role of this East African forest as a carbon store. As the responses to climatic variables were highly group- and species-specific it is likely that climate change will result in a species composition shift, presumably in favour of drought-resistant and heat-tolerating species. 相似文献
135.
136.
Soumitra Paul Chowdhury Kristin Dietel Manuela R?ndler Michael Schmid Helmut Junge Rainer Borriss Anton Hartmann Rita Grosch 《PloS one》2013,8(7)
The soil-borne pathogen Rhizoctonia solani is responsible for crop losses on a wide range of important crops worldwide. The lack of effective control strategies and the increasing demand for organically grown food has stimulated research on biological control. The aim of the present study was to evaluate the rhizosphere competence of the commercially available inoculant Bacillus amyloliquefaciens FZB42 on lettuce growth and health together with its impact on the indigenous rhizosphere bacterial community in field and pot experiments. Results of both experiments demonstrated that FZB42 is able to effectively colonize the rhizosphere (7.45 to 6.61 Log 10 CFU g−1 root dry mass) within the growth period of lettuce in the field. The disease severity (DS) of bottom rot on lettuce was significantly reduced from severe symptoms with DS category 5 to slight symptom expression with DS category 3 on average through treatment of young plants with FZB42 before and after planting. The 16S rRNA gene based fingerprinting method terminal restriction fragment length polymorphism (T-RFLP) showed that the treatment with FZB42 did not have a major impact on the indigenous rhizosphere bacterial community. However, the bacterial community showed a clear temporal shift. The results also indicated that the pathogen R. solani AG1-IB affects the rhizosphere microbial community after inoculation. Thus, we revealed that the inoculant FZB42 could establish itself successfully in the rhizosphere without showing any durable effect on the rhizosphere bacterial community. 相似文献
137.
Tomá? Venit Rastislav Dzijak Al?běta Kalendová Michal Kahle Jana Roho?ková Volker Schmidt Thomas Rülicke Birgit Rathkolb Wolfgang Hans Alexander Bohla Oliver Eickelberg Tobias Stoeger Eckhard Wolf Ali ?nder Yildirim Valérie Gailus-Durner Helmut Fuchs Martin Hrabě de Angelis Pavel Hozák 《PloS one》2013,8(4)
138.
Romana H?ftberger Lidia Sabater Romain Marignier Fahmy Aboul-Enein Rapha?l Bernard-Valnet Helmut Rauschka Anne Ruiz Yolanda Blanco Francesc Graus Josep Dalmau Albert Saiz 《PloS one》2013,8(11)
Cell-based assays (CBA) have increased the sensitivity of the neuromyelitis optica (NMO)-IgG/aquaporin-4-antibody detection compared to classical tissue-based indirect assays. We describe the sensitivity of an optimized immunohistochemistry (IHC-o) to detect NMO-IgG/aquaporin-4-antibody in comparison with that of two CBA: an in-house (CBA-ih) and a commercial (CBA-c) assay (Euroimmun, Germany). Coded serum from 103 patients with definite NMO and 122 inflammatory controls were studied by IHC-o, CBA-ih, and CBA-c. IHC-o used the same protocol described to detect antibodies against cell surface antigens. CBA-ih used live cells transfected with the aquaporin-4-M23-isoform. The sensitivity of the IHC-o was 74.8% (95% confidence interval [CI] 65-83) and was similar to that of the CBA-ih 75.7% (95% CI 66-84) and the CBA-c 73.8% (95% CI 64-82). The specificity of the three assays was 100% (95% CI 97-100). Interassay concordance was high, 100 of 103 samples were coincident in all techniques. The optimized immunohistochemistry proves to be as sensitive and specific as the cell-based assays. This assay extends the available tools for NMO-IgG/aquaporin-4-antibody detection. 相似文献
139.
140.
Helmut D. Hummler Katharina Banke Marla R. Wolfson Giuseppe Buonocore Michael Ebsen Wolfgang Bernhard Dimitrios Tsikas Hans Fuchs 《PloS one》2016,11(2)