Desferal as Improving Agent for Hemoglobin Fructation: Structural and Functional Impacts

Hyperglycemia and advanced glycation end products (AGEs) have considerable effects in diabetic patients. So, the recognition of anti-glycation property of compounds has a substantial benefit. Here, desferal, an iron chelator which is one of the most effective drugs in ??-thalassemia patients, was chosen to explore its effects on the fructation process of hemoglobin (Hb). The results indicated that desferal had a retardation effect on the functional and structural changes of Hb during fructation. It can prevent the AGE and carbonyl formations and helix depletion during the Hb fructation process. Moreover, desferal can preserve peroxidase and esterase activities of fructated Hb similar as native Hb. Therefore, desferal can be introduced as an anti-glycation drug to prevent the AGE formation.     

Archaea in boreal Swedish lakes are diverse,dominated by Woesearchaeota and follow deterministic community assembly

Despite their key role in biogeochemical processes, particularly the methane cycle, archaea are widely underrepresented in molecular surveys because of their lower abundance compared with bacteria and eukaryotes. Here, we use parallel high-resolution small subunit rRNA gene sequencing to explore archaeal diversity in 109 Swedish lakes and correlate archaeal community assembly mechanisms to large-scale latitudinal, climatic (nemoral to arctic) and nutrient (oligotrophic to eutrophic) gradients. Sequencing with universal primers showed the contribution of archaea was on average 0.8% but increased up to 1.5% of the three domains in forest lakes. Archaea-specific sequencing revealed that freshwater archaeal diversity could be partly explained by lake variables associated with nutrient status. Combined with deterministic co-occurrence patterns this finding suggests that ecological drift is overridden by environmental sorting, as well as other deterministic processes such as biogeographic and evolutionary history, leading to lake-specific archaeal biodiversity. Acetoclastic, hydrogenotrophic and methylotrophic methanogens as well as ammonia-oxidizing archaea were frequently detected across the lakes. Archaea-specific sequencing also revealed representatives of Woesearchaeota and other phyla of the DPANN superphylum. This study adds to our understanding of the ecological range of key archaea in freshwaters and links these taxa to hypotheses about processes governing biogeochemical cycles in lakes.     

Seed banks of grazed and ungrazed Baltic seashore meadows

Abstract. The seed banks of three grazed and three ungrazed seashore meadows were studied on the west coast of Finland. 8486 seedlings (mean 13 669 seedlings/m²) germinated from cold-treated samples (n = 343; depth = 10 cm). Most seedlings and species were monocots and perennials. The seed bank flora included 54 dicots vs. 28 monocots and 66 perennials vs. 16 annuals. The most abundant species were Juncus gerardii, Schoenoplectus tabernaemontani, Eleocharis uniglumis, Agrostis stolonifera, Juncus bufonius and Carex nigra, which made up 73% of the seed bank. Numbers of species and seedlings differed between elevation classes. Species richness was highest in elevation class 50–70 cm. The highest seed density occurred in class 20–50 cm. A model for size and species composition of the seed bank in relation to elevation is presented. The seed bank was larger and richer in species in the ungrazed than in the grazed sites, but not so in the upper elevations and closest to the open sea. Grazing reduced the size of the seed bank of Agrostis stolonifera, A. capillaris, Calamagrostis stricta, Elymus repens, Juncus bufonius, Limosella aquatica and Schoenoplectus tabernaemontani, but increased that of J. gerardii. 32 species germinated only from ungrazed samples and 11 species only from grazed ones. Multivariate classification resulted in nine sample groups. The ordination scatter was best explained by the flooding stress variables, elevation, the distance from the water line and the number of helophyte species in samples. 75 species were found both in the seed bank and in the vegetation, but there was a significant lack of resemblance (in the Mantel test) due to over-representation of some species. Eight species occurring only in the seed bank were mainly annuals or biennials (63%); those occurring only in the established vegetation (86 species) were mainly perennials (86%).