全文获取类型
收费全文 | 9717篇 |
免费 | 708篇 |
国内免费 | 3篇 |
专业分类
10428篇 |
出版年
2023年 | 63篇 |
2022年 | 134篇 |
2021年 | 281篇 |
2020年 | 175篇 |
2019年 | 215篇 |
2018年 | 288篇 |
2017年 | 257篇 |
2016年 | 380篇 |
2015年 | 517篇 |
2014年 | 567篇 |
2013年 | 668篇 |
2012年 | 759篇 |
2011年 | 745篇 |
2010年 | 436篇 |
2009年 | 392篇 |
2008年 | 528篇 |
2007年 | 484篇 |
2006年 | 400篇 |
2005年 | 400篇 |
2004年 | 337篇 |
2003年 | 315篇 |
2002年 | 280篇 |
2001年 | 163篇 |
2000年 | 135篇 |
1999年 | 120篇 |
1998年 | 77篇 |
1997年 | 75篇 |
1996年 | 58篇 |
1995年 | 52篇 |
1994年 | 55篇 |
1993年 | 58篇 |
1992年 | 72篇 |
1991年 | 73篇 |
1990年 | 67篇 |
1989年 | 55篇 |
1988年 | 47篇 |
1987年 | 52篇 |
1986年 | 68篇 |
1985年 | 48篇 |
1984年 | 51篇 |
1983年 | 41篇 |
1982年 | 44篇 |
1981年 | 31篇 |
1980年 | 32篇 |
1979年 | 29篇 |
1978年 | 32篇 |
1977年 | 34篇 |
1976年 | 22篇 |
1975年 | 34篇 |
1974年 | 34篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
81.
Juliano Silva Lima Igor David da Costa Ilana Rosental Zalmon 《Zeitschrift fur angewandte Ichthyologie》2021,37(2):337-341
The present work provides length-weight relationships (LWRs) for fish species captured around artificial offshore reef deployed 5 km from Guaxindiba Port, northern Rio de Janeiro, Brazil. The fish were captured during two sampling periods each year between 1996 and 2017 using bottom gill nets (25 mlength × 3 mdepth; 30 mm mesh). The fish were kept on ice and the biometric data, including total length (cm) and total wet weight (g) were determined in the laboratory. A total of 16 species belonging to 10 families were analyzed with Sciaenidae being the species richest family (5 spp) in the samples. The new value of LWRs for Sphyraena guachancho and new maximum sizes recorded for seven species highlight the scarcity of information on biological aspects of South America coastal fishes. These LWRs should assist fisheries scientist and managers to complement their further studies of population parameters to improve management decisions. 相似文献
82.
Hydrobiologia - Mussels provide important ecological functions in freshwater ecosystems but the associations between Amazonian mussels, macroinvertebrate assemblage and habitat quality remain... 相似文献
83.
Leduc Antoine O. H. C. Costa Jessica S. Oliveira do Nascimento Silva Renata R. Winandy Gabrielle S. M. de Araújo Carlos B. 《Journal of Ethology》2021,39(2):225-234
Journal of Ethology - Noise pollution may impair the cognitive performances of several animal species, producing suboptimal behavioral responses. Involuntary shifts in attention from noise... 相似文献
84.
Calmasini Fabiano B. Alexandre Eduardo C. Oliveira Mariana G. Silva Fábio H. Soares António G. Costa Soraia K.P. Antunes Edson 《Journal of physiology and biochemistry》2021,77(4):557-564
Journal of Physiology and Biochemistry - Lipopolysaccharide (LPS) is a component of gram-negative bacteria wall that elicits inflammatory response in the host through the toll-like receptor 4... 相似文献
85.
Woyneshet Gelaye Nana Aba Williams Stella Kepha Augusto Messa Junior Pedro Emanuel Fleitas Helena Marti-Soler Destaw Damtie Sissay Menkir Alejandro J. Krolewiecki Lisette van Lieshout Wendemagegn Enbiale 《PLoS neglected tropical diseases》2021,15(2)
BackgroundSoil-transmitted helminths (STH) are common in low and middle income countries where there is lack of access to clean water and sanitation. Effective diagnosis and treatment are essential for the control of STH infections. However, among STH parasites, Strongyloides stercoralis is the most neglected species, both in diagnostics and control strategies. Diagnostic methods cover different approaches, each with different sensitivities and specificities, such as serology, molecular techniques and microscopy based techniques. Of the later, the Baermann technique is the most commonly used procedure. In the literature, several ways have been described to perform the Baermann method, which illustrates the overall lack of a ‘(gold) reference standard’ method for the diagnosis of S. stercoralis infection. In this study we have evaluated the performance of three Baermann techniques in order to improve the reference standard for the microscopic diagnosis of S. stercoralis infection thereby facilitating individual case detection, mapping of the disease and proper evaluation of treatment responses.Methods/Principal findingsA community based cross sectional study was conducted at Zenzelima, Bahir Dar Zuria Ethiopia. A total of 437 stool samples were collected and analyzed by the following procedures: conventional Baermann (CB), modified Baermann (MB), and modified Baermann with charcoal pre-incubation (MBCI). The diagnostic sensitivity and Negative Predictive Value (NPV) of each technique was calculated using the combination of all the three techniques as a composite reference standard. Our result indicated that larvae of S. stercoralis were detected in 151 (34.6%) stool samples. The prevalence of S. stercoralis infection based on the three diagnostic methods was 9.6%, 8.0%, and 31.3% by CB, MB, and MBCI respectively. The sensitivity and NPV for CB, MB, and MBCI were 26.7% and 70.8%, 22.1% and 69.6%, and 87.0% and 93.2%, respectively. The MBCI showed significant difference (P- value = <0.001) in the sensitivity and NPV values when compared with CB and MB values. The agreement between CB, MB, and MBCI with the composite reference standard was 31.8%, 26.7%, 89.6%, respectively.Conclusion/SignificanceOur results suggest the superior performance of MBCI. It is relatively easy to implement, simple to perform and comparatively cheaper. The CB is by far the commonly used method in routine diagnostic although this technique significantly underestimates the true burden of the disease and thereby contributing to the exclusion of S. stercoralis from the control strategies. Therefore, MBCI is recommended as a routine microscopy-based diagnostic test for S. stercoralis infection, particularly in settings where molecular procedures are not available. 相似文献
86.
87.
Mário S. Diniz Hugo M. Santos Pedro M. Costa Isabel Peres Maria H. Costa José L. Capelo 《Biomarkers》2013,18(6):589-598
The main objective of this work was to evaluate arsenic effects on metallothionein (MT) induction by exposing a freshwater Asiatic clam (Corbicula fluminea) to different concentrations of this metalloid. The presence of MT-like proteins was detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis and compared with a standard rabbit MT. In addition, the polarographic response showed good correspondence between standard MT and MT-like curves from C. fluminea, allowing MT quantification. The results show that clams exposed to different concentrations of arsenic are able to induce significant levels of MTs. Although variability was found in MT induction, significant differences in MT levels were found after 28 days of exposure in all treatments in comparison with the controls, suggesting that exposure to arsenic induced MT-like proteins in C. fluminea. 相似文献
88.
89.
Sílvia Castro Victoria Ferrero Joana Costa Ana João Sousa Mariana Castro Luis Navarro João Loureiro 《Biological invasions》2013,15(8):1863-1875
Oxalis pes-caprae, a tristylous flowering plant native to South Africa, is described in the western Mediterranean basin as an asexual—only 5x short-styled morph (5x S-morph) invasive weed losing all mating partners after introduction. The objective of this study was to reassess the patterns of floral morph and cytotype distribution and the sexual reproduction ability in this invaded range. For that, floral morph and cytotype composition were evaluated in 39 populations of O. pes-caprae in a methodical sampling. The reproductive success of natural populations was assessed as fruit and seed production and seed germination for all floral morphs and cytotypes detected. Self- and morph-incompatibility were also studied with controlled hand pollinations. A remarkable diversity in floral morph and cytotype composition was observed. Furthermore, we observed successful sexual reproduction in several localities across the surveyed area. The S-morph is still dominant in this invaded area, and although it was mostly 5x, an additional cytotype (4x) was also recorded. Records of both a mid-styled morph (M-morph) and an area with trimorphic populations of this species are reported here for the first time in the invasive range of the Mediterranean basin. The long-styled morph appears to occur randomly across the surveyed area, while the M-morph is concentrated mainly in Estremadura province (Portugal), where a breakdown in the incompatibility system was observed. These distribution patterns may result from events of sexual reproduction after incompatibility breakdown and/or from multiple introduction events from the native area. The ability to reproduce sexually, undetected so far, may have important impacts in the population dynamics and major consequences for the adaptation and selection potential of O. pes-caprae in this invaded area. 相似文献
90.
Victoria Ferrero Sílvia Castro Joana Costa Paola Acuña Luis Navarro João Loureiro 《Biological invasions》2013,15(10):2347-2358
Removal of invasive species often benefits biological diversity allowing ecosystems’ recovery. However, it is important to assess the functional roles that invaders may have established in their new areas to avoid unexpected results from species elimination. Invasive animal-pollinated plants may affect the plant–pollination interactions by changing pollinator availability and/or behaviour in the community. Thus, removal of an invasive plant may have important effects on pollinator community that may then be reflected positive or negatively on the reproductive success of native plants. The objective of this study was to assess the effect of removing Oxalis pes-caprae, an invasive weed widely spread in the Mediterranean basin, on plant–pollinator interactions and on the reproductive success of co-flowering native plants. For this, a disturbed area in central Portugal, where this species is highly abundant, was selected. Visitation rates, natural pollen loads, pollen tube growth and natural fruit set of native plants were compared in the presence of O. pes-caprae and after manual removal of their flowers. Our results showed a highly resilient pollination network but also revealed some facilitative effects of O. pes-caprae on the reproductive success of co-flowering native plants. Reproductive success of the native plants seems to depend not only on the number and diversity of floral visitors, but also on their efficiency as pollinators. The information provided on the effects of invasive species on the sexual reproductive success of natives is essential for adequate management of invaded areas. 相似文献