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941.
William?A.?McLaughlin Daniel?W.?Kulp Joanna?de la?Cruz Xiang-Jun?Lu Catherine?L.?Lawson Helen?M.?BermanEmail author 《Journal of structural and functional genomics》2005,5(4):255-265
A classification model of a DNA-binding protein chain was created based on identification of alpha helices within the chain likely to bind to DNA. Using the model, all chains in the Protein Data Bank were classified. For many of the chains classified with high confidence, previous documentation for DNA-binding was found, yet no sequence homology to the structures used to train the model was detected. The result indicates that the chain model can be used to supplement sequence based methods for annotating the function of DNA-binding. Four new candidates for DNA-binding were found, including two structures solved through structural genomics efforts. For each of the candidate structures, possible sites of DNA-binding are indicated by listing the residue ranges of alpha helices likely to interact with DNA. 相似文献
942.
Genomes are housed within cell nuclei as individual chromosome territories. Nuclei contain several architectural structures
that interact and influence the genome. In this review, we discuss how the genome may be organised within its nuclear environment
with the position of chromosomes inside nuclei being either influenced by gene density or by chromosomes size. We compare
interphase genome organisation in diverse species and reveal similarities and differences between evolutionary divergent organisms.
Genome organisation is also discussed with relevance to regulation of gene expression, development and differentiation and
asks whether large movements of whole chromosomes are really observed during differentiation. Literature and data describing
alterations to genome organisation in disease are also discussed. Further, the nuclear structures that are involved in genome
function are described, with reference to what happens to the genome when these structures contain protein from mutant genes
as in the laminopathies.
Review related to the 15th International Chromosome Conference (ICC XV), held in September 2004, Brunel University, London,
UK 相似文献
943.
944.
Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies 总被引:13,自引:0,他引:13
RNA interference (RNAi) is an important means of eliminating mRNAs, but the intracellular location of RNA-induced silencing complex (RISC) remains unknown. We show here that Argonaute 2, a key component of RISC, is not randomly distributed but concentrates in mRNA decay centres that are known as cytoplasmic bodies. The localization of Argonaute 2 in decay centres is not altered by the presence or absence of small interfering RNAs or their targeted mRNAs. However, RNA is required for the integrity of cytoplasmic bodies because RNase eliminates Argonaute 2 localization. In addition, Argonaute 1, another Argonaute family member, is concentrated in cytoplasmic bodies. These results provide new insight into the mechanism of RNAi function. 相似文献
945.
946.
In the September 9th issue of Cell, Mikeladze-Dvali et al. show that cell fate decisions needed for color vision are dependent on a bistable negative feedback loop between genes previously implicated in cell proliferation (warts) and growth (melted). 相似文献
947.
BackgroundMathematical models have become invaluable management tools for epidemiologists, both shedding light on the mechanisms underlying observed dynamics as well as making quantitative predictions on the effectiveness of different control measures. Here, we explain how substantial biases are introduced by two important, yet largely ignored, assumptions at the core of the vast majority of such models.ConclusionThis work aims to highlight that, when developing models for public health use, we need to pay careful attention to the intrinsic assumptions embedded within classical frameworks. 相似文献
948.
Leung KY Lescuyer P Campbell J Byers HL Allard L Sanchez JC Ward MA 《Proteomics》2005,5(12):3040-3044
A novel strategy consisting of cleavable Isotope-Coded Affinity Tag (cICAT) combined with MASCOT Distiller was evaluated as a tool for the quantification of proteins in "abnormal" patient plasma, prepared by pooling samples from patients with acute stroke. Quantification of all light and heavy cICAT-labelled peptide ion pairs was obtained using MASCOT Distiller combined with a proprietary software. Peptides displaying differences were selected for identification by MS. These preliminary results show the promise of our approach to identify potential biomarkers. 相似文献
949.
Malakhova ML Malinina L Pike HM Kanack AT Patel DJ Brown RE 《The Journal of biological chemistry》2005,280(28):26312-26320
Mammalian glycolipid transfer proteins (GLTPs) facilitate the selective transfer of glycolipids between lipid vesicles in vitro. Recent structural determinations of the apo- and glycolipid-liganded forms of human GLTP have provided the first insights into the molecular architecture of the protein and its glycolipid binding site (Malinina, L., Malakhova, M. L., Brown, R. E., and Patel, D. J. (2004) Nature 430, 1048-1053). In the present study, we have evaluated the functional consequences of point mutation of the glycolipid liganding site of human GLTP within the context of a carrier-based mechanism of glycolipid intermembrane transfer. Different approaches were developed to rapidly and efficiently assess the uptake and release of glycolipid by GLTP. They included the use of glass-immobilized, glycolipid films to load GLTP with glycolipid and separation of GLTP/glycolipid complexes from vesicles containing glycolipid (galactosylceramide or lactosylceramide) or from monosialoganglioside dispersions by employing nickel-nitrilotriacetic acid-based affinity or gel filtration strategies. Point mutants of the sugar headgroup recognition center (Trp-96, Asp-48, Asn-52) and of the ceramide-accommodating hydrophobic tunnel (Phe-148, Phe-183, Leu-136) were analyzed for their ability to acquire and release glycolipid ligand. Two manifestations of point mutation within the liganding site were apparent: (i) impaired formation of the GLTP/glycolipid complex; (ii) impaired acquisition and release of bound glycolipid by GLTP. The results are consistent with a carrier-based mode of GLTP action to accomplish the intermembrane transfer of glycolipid. Also noteworthy was the inefficient release of glycolipid by wtGLTP into phosphatidylcholine acceptor vesicles, raising the possibility of a function other than intermembrane glycolipid transfer in vivo. 相似文献
950.