排序方式: 共有61条查询结果,搜索用时 390 毫秒
41.
Hantschel O Wiesner S Güttler T Mackereth CD Rix LL Mikes Z Dehne J Görlich D Sattler M Superti-Furga G 《Molecular cell》2005,19(4):461-473
The Bcr-Abl tyrosine kinase causes different forms of leukemia in humans. Depending on its position within the cell, Bcr-Abl differentially affects cellular growth. However, no structural and molecular details for the anticipated localization determinants are available. We present the NMR structure of the F-actin binding domain (FABD) of Bcr-Abl and its cellular counterpart c-Abl. The FABD forms a compact left-handed four-helix bundle in solution. We show that the nuclear export signal (NES) previously reported in this region is part of the hydrophobic core and nonfunctional in the intact protein. In contrast, we could identify the critical residues of helix alphaIII that are responsible for F-actin binding and cytoskeletal association. We propose that these interactions represent a major determinant for both Bcr-Abl and c-Abl localization. 相似文献
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D. Mathioudakis J. Engel I. D. Welters M. G. Dehne R. Matejec H. Harbach M. Henrich T. Schwandner M. Fuchs K. Weism��ller G. J. Scheffer J?rg M��hling 《Amino acids》2011,40(4):1077-1090
For the first time the immunonutritional role of pyruvate on neutrophils (PMN), free ??-keto and amino acid profiles, important reactive oxygen species (ROS) produced [superoxide anion (O2 ?), hydrogen peroxide (H2O2)] as well as released myeloperoxidase (MPO) acitivity has been investigated. Exogenous pyruvate significantly increased PMN pyruvate, ??-ketoglutarate, asparagine, glutamine, aspartate, glutamate, arginine, citrulline, alanine, glycine and serine in a dose as well as duration of exposure dependent manner. Moreover, increases in O2 ? formation, H2O2-generation and MPO acitivity in parallel with intracellular pyruvate changes have also been detected. Regarding the interesting findings presented here we believe, that pyruvate fulfils considerably the criteria for a potent immunonutritional molecule in the regulation of the PMN dynamic ??-keto and amino acid pools. Moreover it also plays an important role in parallel modulation of the granulocyte-dependent innate immune regulation. Although further research is necessary to clarify pyruvate??s sole therapeutical role in critically ill patients?? immunonutrition, the first scientific successes seem to be very promising. 相似文献
45.
Epigenetic processes elicit changes in gene expression by modifying DNA bases or histone side chains without altering DNA sequences. Recently discovered Jumonji histone demethylases (JHDMs) affect gene expression by demethylating lysine residues of histone tails. JHDMs belong to a family of dioxygenases and share similarities with prolyl hydroxylases (PHDs). Therefore, we investigated the influence of hypoxia in macrophages on histone methylation. All JHDM family members JMJD1A–C and JMJD2A–D are expressed in macrophages. Thus, we analyzed the methylation status of histone H3 residues not only under hypoxia but also after treatment with the dioxygenase-inhibitors DMOG, NO and ROS. Western analysis revealed increased methylations in H3K9me2/me3 and H3K36me3 at pO2 below 3%, DMOG, NO and ROS treatment. Chromatin immunoprecipitation (ChIP) assays demonstrated increased repressive marks H3K9me2 and H3K9me3 in specific promoter regions of the chemokine Ccl2 and the chemokine receptors Ccr1 and Ccr5, which correlated with a downregulation of their mRNA expression under hypoxic conditions. In contrasts, the hypoxia-inducible factor (HIF) target gene adrenomedullin (ADM) mRNA was upregulated and no increase in its histone modification was observed. We suggest that hypoxia and a concomitant loss of JHDM activity increases H3K9 methylation and decreases chemokine expression. 相似文献
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In 1998–2000 a monitoring of the spectrum of Fusarium species on winter wheat was carried out in the Rhineland. The epidemic spread ofFusarium spp. on wheat plants during growing season was investigated as well as the grain contamination after harvest.F avenaceum was the Fusarium species isolated most frequently followed byF culmorum, F poae andF graminearum. Microdochium nivale occurred considerably only in 1998. Both, susceptibility and plant height of the cultivars were correlated to the incidence of Fusarium species /M nivale on harvested kernels; interactions with cropping intensities were detected. The incidence ofF poae seemed to be independent of the cultivar-specific Fusarium susceptibility. Despite the lack of disease symptoms, between growth stages 45–85 mycelium ofFusarium spp. was detectable in the leaves as well as conidia on the leaf surfaces. 相似文献
47.
Mühling J Nickolaus KA Halabi M Fuchs M Krüll M Engel J Wolff M Matejec R Langefeld TW Welters ID Menges T Dehne MG Sablotzki A Hempelmann G 《Amino acids》2005,29(3):289-300
Summary. The objective of this study was to determine the dose as well as duration of exposure-dependent effects of L-alanyl-L-glutamine,
arginine or taurine on polymorphonuclear neutrophil (PMN) free α-keto acid profiles and, in a parallel study, on PMN immune
functions. Exogenous L-alanyl-L-glutamine significantly increased PMN α-ketoglutarate, pyruvate PMN superoxide anion (O2−) generation, hydrogen peroxide (H2O2) formation and released myeloperoxidase (MPO) activity. Arginine also led to significant increases in α-ketoglutarate, pyruvate,
MPO release and H2O2 generation. Formation of O2− on the other hand was decreased by arginine. Incubation with taurine resulted in lower intracellular pyruvate and α-ketobutyrate
levels, decreased O2− and H2O2 formation and a concomitant significantly increased MPO activity. We therefore believe that considerable changes in PMN free-α-keto-acid
profiles, induced for example by L-alanyl-L-glutamine, arginine or taurine, may be one of the determinants in cell nutrition
that considerably modulates the immunological competence of PMN. 相似文献
48.
In a long-lasting field experiment at the Research Station Hanninghof at Dülmen (Westphalia), laid out in 1971, the inoculum
potential (IP) of vesicular-arbuscular mycorrhizal (VAM) fungi of soil from winter wheat in a continuous monoculture (CM)
and in a four-years cereal crop rotation (CR, winter wheat, winter barley, winter rye, oats) was investigated from 1980 to
1982. The influence of green manure and two levels of introgen fertilization (100 and 200 kg ha−1) on VAM-IP was also assessed. It turned out that VAM is frequent also in intensively cultivated soils, but varies this with
the crop rotation system and form and intensity of fertilization. Compared to CR the VAM-IP was significantly reduced in CM.
A cumulation of this effect, with a particularly strong decrease of VAM occurred when other factors unfavourable for the symbiosis
coincided in CM. Among these factors were the use of non-mycorrhizalRaphanus sativus andBrassica napus for green manure together with a N-fertilization of 200 kg ha−1. On the other hand green manure and the higher N-fertilization resulted in less disturbance of the VAM-IP in the CR. A correlation
between VAM-IP in the soil and of wheat yields could neither be observed in the CR nor in the CM. 相似文献
49.
The inoculation of wheat ears with 27 isolates ofFusarium culmorum in growth stage 65 reduced 1000-grain weights by 14 to 61%. For the phytopathological characterisation of isolates the virulence on primary wheat leaves and the growth rate an potato-dextrose-agar were assessed. Deoxynivalenol-producing isolates ofF. culmorum reduced the 1000-grain weight more than nivalenol-producing isolates. 相似文献
50.
Tilo Dehne Camilla Karlsson Jochen Ringe Michael Sittinger Anders Lindahl 《Arthritis research & therapy》2009,11(5):R133-14