全文获取类型
收费全文 | 3139篇 |
免费 | 264篇 |
国内免费 | 32篇 |
专业分类
3435篇 |
出版年
2021年 | 26篇 |
2020年 | 22篇 |
2019年 | 23篇 |
2018年 | 29篇 |
2017年 | 33篇 |
2016年 | 49篇 |
2015年 | 73篇 |
2014年 | 92篇 |
2013年 | 128篇 |
2012年 | 179篇 |
2011年 | 164篇 |
2010年 | 99篇 |
2009年 | 91篇 |
2008年 | 139篇 |
2007年 | 140篇 |
2006年 | 147篇 |
2005年 | 138篇 |
2004年 | 135篇 |
2003年 | 148篇 |
2002年 | 178篇 |
2001年 | 50篇 |
2000年 | 43篇 |
1999年 | 51篇 |
1998年 | 57篇 |
1997年 | 50篇 |
1996年 | 57篇 |
1995年 | 51篇 |
1994年 | 56篇 |
1993年 | 40篇 |
1992年 | 34篇 |
1991年 | 31篇 |
1990年 | 34篇 |
1989年 | 35篇 |
1988年 | 25篇 |
1987年 | 28篇 |
1986年 | 35篇 |
1985年 | 35篇 |
1984年 | 48篇 |
1983年 | 34篇 |
1982年 | 44篇 |
1981年 | 42篇 |
1980年 | 36篇 |
1979年 | 20篇 |
1978年 | 25篇 |
1977年 | 23篇 |
1976年 | 23篇 |
1975年 | 21篇 |
1974年 | 29篇 |
1970年 | 22篇 |
1968年 | 22篇 |
排序方式: 共有3435条查询结果,搜索用时 15 毫秒
41.
42.
Lentiviral vectors have been used for gene transfer into the liver but their ability to efficiently transduce quiescent hepatocytes
remains controversial. Lentivirus-mediated gene transfer is more efficient in cycling cells. We determine the effect of H-IL6
in the lentiviral transduction. The lentiviral vector was used to transduce HepG2 cells and mice liver cells, previously treated
with H-IL6. The highest transduction level was observed in HepG2 cells treated with 30 ng/mL H-IL6 and in the mice that received
4 μg H-IL6. Our results suggest that H-IL6 is an inducer of lentiviral gene transfer into the liver cells without any toxicity. 相似文献
43.
44.
Effects of grass species and grass growth on atmospheric nitrogen deposition to a bog ecosystem surrounded by intensive agricultural land use 下载免费PDF全文
Miriam Hurkuck Christian Brümmer Karsten Mohr Oliver Spott Reinhard Well Heinz Flessa Werner L. Kutsch 《Ecology and evolution》2015,5(13):2556-2571
We applied a 15N dilution technique called “Integrated Total Nitrogen Input” (ITNI) to quantify annual atmospheric N input into a peatland surrounded by intensive agricultural practices over a 2-year period. Grass species and grass growth effects on atmospheric N deposition were investigated using Lolium multiflorum and Eriophorum vaginatum and different levels of added N resulting in increased biomass production. Plant biomass production was positively correlated with atmospheric N uptake (up to 102.7 mg N pot−1) when using Lolium multiflorum. In contrast, atmospheric N deposition to Eriophorum vaginatum did not show a clear dependency to produced biomass and ranged from 81.9 to 138.2 mg N pot−1. Both species revealed a relationship between atmospheric N input and total biomass N contents. Airborne N deposition varied from about 24 to 55 kg N ha−1 yr−1. Partitioning of airborne N within the monitor system differed such that most of the deposited N was found in roots of Eriophorum vaginatum while the highest share was allocated in aboveground biomass of Lolium multiflorum. Compared to other approaches determining atmospheric N deposition, ITNI showed highest airborne N input and an up to fivefold exceedance of the ecosystem-specific critical load of 5–10 kg N ha−1 yr−1. 相似文献
45.
46.
Dolashki A Velkova L Atanasov B Voelter W Stevanovic S Schwarz H Di Muro P Dolashka-Angelova P 《Biochimica et biophysica acta》2008,1784(11):1617-1624
We have studied the stability and reassociation behaviour of native molecules of Rapana venosa hemocyanin and its two subunits, termed RvH1 and RvH2. In the presence of different concentrations of Ca(2+) and Mg(2+) ions and pH values, the subunits differ not only in their reassociation behaviour, but also in their formation of helical tubules and multidecamers. RvH1 revealed a greater stability at higher pH values compared to RvH2. Overall, the stability of reassociated RvH and its structural subunits was found to be pH-dependent. The increasing stability of native Hc and its subunits, shown by pH-induced CD transitions (acid and alkaline denaturation), can be explained with the formation of quaternary structure. The absence of a Cotton effect at temperatures 20-40 degrees C in the pH-transition curves of RvH2 indicates that this subunit is stabilized by additional "factors", e.g.: non-ionic/hydrophobic stabilization and interactions of carbohydrate moieties. A similar behaviour was observed for the T-transition curves in a wide pH interval for RvH and its structural subunits. At higher temperatures, many of the secondary structural elements are preserved especially at neutral pH, even at extreme high temperatures above 90 degrees C the protein structures resemble a "globule state". 相似文献
47.
48.
Aleksey V Zimin Alaina Shumate Ida Shinder Jakob Heinz Daniela Puiu Mihaela Pertea Steven L Salzberg 《Genetics》2022,220(2)
Until 2019, the human genome was available in only one fully annotated version, GRCh38, which was the result of 18 years of continuous improvement and revision. Despite dramatic improvements in sequencing technology, no other genome was available as an annotated reference until 2019, when the genome of an Ashkenazi individual, Ash1, was released. In this study, we describe the assembly and annotation of a second individual genome, from a Puerto Rican individual whose DNA was collected as part of the Human Pangenome project. The new genome, called PR1, is the first true reference genome created from an individual of African descent. Due to recent improvements in both sequencing and assembly technology, and particularly to the use of the recently completed CHM13 human genome as a guide to assembly, PR1 is more complete and more contiguous than either GRCh38 or Ash1. Annotation revealed 37,755 genes (of which 19,999 are protein coding), including 12 additional gene copies that are present in PR1 and missing from CHM13. Fifty-seven genes have fewer copies in PR1 than in CHM13, 9 map only partially, and 3 genes (all noncoding) from CHM13 are entirely missing from PR1. 相似文献
49.
Schlossmacher U Wiens M Schröder HC Wang X Jochum KP Müller WE 《The FEBS journal》2011,278(7):1145-1155
Silicateins are unique enzymes of sponges (phylum Porifera) that template and catalyze the polymerization of nanoscale silicate to siliceous skeletal elements. These multifunctional spicules are often elaborately shaped, with complex symmetries. They carry an axial proteinaceous filament, consisting of silicatein and the scaffold protein silintaphin-1, which guides silica deposition and subsequent spicular morphogenesis. In vivo, the synthesis of the axial filament very likely proceeds in three steps: (a) assembly of silicatein monomers to form one pentamer; (b) assembly of pentamers to form fractal-like structures; and finally (c) assembly of fractal-like structures to form filaments. The present study was aimed at exploring the effect of self-assembled complexes of silicatein and silintaphin-1 on biosilica synthesis in vitro. Hence, in a comparative approach, recombinant silicatein and recombinant silintaphin-1 were used at different stoichiometric ratios to form axial filaments and to synthesize biosilica. Whereas recombinant silicatein-α reaggregates to randomly organized structures, coincubation of silicatein-α and silintaphin-1 (molecular ratio 4 : 1) resulted in synthetic filaments via fractal-like patterned self-assemblies, as observed by electron microscopy. Concurrently, owing to the concerted action of both proteins, the enzymatic activity of silicatein-α strongly increased by 5.3-fold (with the substrate tetraethyl orthosilicate), leading to significantly enhanced synthesis of biosilica. These results indicate that silicatein-α-mediated biosilicification depends on the concomitant presence of silicatein-α and silintaphin-1. Accordingly, silintaphin-1 might not only enhance the enzymatic activity of silicatein-α, but also accelerate the nonenzymatic polycondensation of the silica product before releasing the fully synthesized biosiliceous polymer. 相似文献
50.