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91.
Christian Heinen Jed Long Stuart H. Ralston Mark S. Searle Nico P. Dantuma 《FEBS letters》2010,584(8):1585-4674
We show that the ubiquitin-associated domain (UBA) of human p62/sequestosome-1 (SQSTM1) can delay degradation of proteasome substrates in yeast. Taking advantage of naturally occurring mutant UBA domains that are linked to Paget’s disease of bone (PDB), we found that three of the four mutant UBA domains tested in this study were able to inhibit proteasomal degradation, albeit not to the same extent as the wild-type domain. Interestingly, the stability measured as the fraction of folded protein, and not the ubiquitin binding properties, of the PDB-associated UBA domains correlated with their protective effects. These data suggest that the protective effect of UBA domains depends on their structural integrity rather than ubiquitin binding capabilities. 相似文献
92.
Immunoquantitative Real-Time PCR for Detection and Quantification of Staphylococcus aureus Enterotoxin B in Foods 下载免费PDF全文
Andreja Rajkovic Benaissa El Moualij Mieke Uyttendaele Philippe Brolet Willy Zorzi Ernst Heinen Ellen Foubert Johan Debevere 《Applied microbiology》2006,72(10):6593-6599
A real-time immunoquantitative PCR (iqPCR) method for detection of Staphylococcus aureus enterotoxin B (SEB) was developed and evaluated using both pure cultures and foods. The assay consisted of immunocapture of SEB and real-time PCR amplification of the DNA probe linked to the detection antibody. iqPCR was compared to an in-house enzyme-linked immunosorbent assay (ELISA) using the same couple of capture-detection antibodies and to commercial kits for detection of S. aureus enterotoxins (SE). The iqPCR was approximately 1,000 times more sensitive (<10 pg ml−1) than the in-house ELISA and had a dynamic range of approximately 10 pg ml−1 to approximately 30,000 pg ml−1. iqPCR was not inhibited by any of the foods tested and was able to detect SEB present in these foods. No cross-reactivity with SE other than SEB was observed. Application of iqPCR for detection of SEB in cultures of S. aureus revealed the onset of SEB production after 4 h of incubation at 22, 37, and 42°C, which was in the first half of the exponential growth phase. The total amounts of SEB produced by the two strains tested were larger at 42°C than at 37°C and were strain dependent. 相似文献
93.
A model wheat cultivar for transformation to improve resistance to Fusarium Head Blight 总被引:2,自引:2,他引:0
Fusarium head blight (FHB), caused primarily by Fusarium graminearum, is a major disease problem in wheat (Triticum aestivum). Genetic engineering holds significant potential to enhance FHB resistance in wheat. Due to the requirement of screening
for FHB resistance on flowers at anthesis, the number of screens carried out in a year is limited. Our objective was to evaluate
the feasibility of using the rapid-maturing dwarf wheat cultivar Apogee as an alternative genotype for transgenic FHB resistance
research. Our transformation efficiency (number of transgenic plants/number of embryos) for Apogee was 1.33%. Apogee was also
found to exhibit high FHB susceptibility and reached anthesis within 4 weeks. Interestingly, microsatellite marker haplotype
analysis of the chromosome 3BS FHB resistant quantitative trait locus (QTL) region indicated that this region maybe deleted
in Apogee. Our results indicate that Apogee is particularly well suited for accelerating transgenic FHB resistance research
and transgenic wheat research in general.
C.A. Mackintosh and D.F. Garvin contributed equally to the article and should be considered co-first authors 相似文献
94.
Expansins are involved in the formation of nematode-induced syncytia in roots of Arabidopsis thaliana 总被引:1,自引:0,他引:1
95.
Andreas Pomschar Inga Koerte Sang Lee Ruediger P. Laubender Andreas Straube Florian Heinen Birgit Ertl-Wagner Noam Alperin 《PloS one》2013,8(2)
Purpose
To compare venous drainage patterns and associated intracranial hydrodynamics between subjects who experienced mild traumatic brain injury (mTBI) and age- and gender-matched controls.Methods
Thirty adult subjects (15 with mTBI and 15 age- and gender-matched controls) were investigated using a 3T MR scanner. Time since trauma was 0.5 to 29 years (mean 11.4 years). A 2D-time-of-flight MR-venography of the upper neck was performed to visualize the cervical venous vasculature. Cerebral venous drainage through primary and secondary channels, and intracranial compliance index and pressure were derived using cine-phase contrast imaging of the cerebral arterial inflow, venous outflow, and the craniospinal CSF flow. The intracranial compliance index is the defined as the ratio of maximal intracranial volume and pressure changes during the cardiac cycle. MR estimated ICP was then obtained through the inverse relationship between compliance and ICP.Results
Compared to the controls, subjects with mTBI demonstrated a significantly smaller percentage of venous outflow through internal jugular veins (60.9±21% vs. controls: 76.8±10%; p = 0.01) compensated by an increased drainage through secondary veins (12.3±10.9% vs. 5.5±3.3%; p<0.03). Mean intracranial compliance index was significantly lower in the mTBI cohort (5.8±1.4 vs. controls 8.4±1.9; p<0.0007). Consequently, MR estimate of intracranial pressure was significantly higher in the mTBI cohort (12.5±2.9 mmHg vs. 8.8±2.0 mmHg; p<0.0007).Conclusions
mTBI is associated with increased venous drainage through secondary pathways. This reflects higher outflow impedance, which may explain the finding of reduced intracranial compliance. These results suggest that hemodynamic and hydrodynamic changes following mTBI persist even in the absence of clinical symptoms and abnormal findings in conventional MR imaging. 相似文献96.
Moermans C Heinen V Nguyen M Henket M Sele J Manise M Corhay JL Louis R 《Cytokine》2011,56(2):298-304
Background
Chronic obstructive pulmonary disease (COPD) is a chronic airway inflammatory disease caused by repeated exposure to noxious gases or particles. It is now recognized that the disease also features systemic inflammation. The purpose of our study was to compare airway and systemic inflammation in COPD to that seen in healthy subjects and to relate the inflammation with the disease severity.Methods
Ninety-five COPD patients, encompassing the whole severity spectrum of the disease, were recruited from our outpatient clinic and rehabilitation center and compared to 33 healthy subjects. Induced sputum and blood samples were obtained for measurement of inflammatory cell count. Interleukin (IL)-4, IL-6, IL-10, TNF-α and IFN-γ produced by 24 h sputum and blood cell cultures were measured.Results
Compared to healthy subjects, COPD exhibited a prominent airway neutrophilic inflammation associated with a marked IL-10, IL-6 and TNF-α release deficiency that contrasted with a raised IFN-γ production. Neutrophilic inflammation was also prominent at blood level together with raised production of IFN-γ, IL-10 and TNF-α. Furthermore, sputum neutrophilia correlated with disease severity assessed by GOLD stages. Likewise the extent of TNF-α release from blood cells also positively correlated with the disease severity but negatively with that of sputum cell culture. Blood release of TNF-α and IL-6 negatively correlated with body mass index. Altogether, our results showed a significant relationship between cellular marker in blood and sputum but poor relationship between local and systemic release of cytokines.Conclusions
COPD is characterized by prominent neutrophilic inflammation and raised IFN-γ production at both bronchial and systemic level. Overproduction of TNF-α at systemic level correlates with disease severity and inversely with body mass index. 相似文献97.
98.
The large N-linked oligosaccharides released from Schizosaccharomyces pombe
by endo-beta-N-acetylglucosaminidase H were examined to determine how the
negatively chargedpyruvylated galactoses present (Gemmill,T.R., and
Trimble,R.B., 1996, J. Biol. Chem ., 271, 25945-25949) were attached to the
oligosaccharide chains. Binding of biotinylated human serum amyloid P and
peanut agglutinin to native and depyruvylated S.pombe glycoproteins,
respectively, indicated that the pyruvylated epitope was likely to be in
the beta configuration. Examination by high- field 1H NMR of whole glycans
and a disaccharide fragment released from them on partial acid hydrolysis
showed that the pyruvylated galactose species was in fact beta1,3-linked to
a second galactose, and this occurred an average of five to six times on
nominal Gal57Man64GlcNAc N- glycans. The pyruvate-2,(4,6)Gal-beta1,3Gal
epitope is chemically similar to acetaldehyde-Galbeta1,3Gal groups found on
the glycoproteins from Paramyxovirus-infected bovine kidney cells (Prehm,
P., Scheid,A. and Choppin,P.W. ,1979, J. Biol. Chem ., 254, 9669-9677). The
1:1 stoichiometry between pyruvate and beta-linked galactose in these
S.pombe glycans indicates that either pyruvate addition to terminal
beta1,3Gal is highly efficient or that pyruvylated Gal is transferred en
bloc to alpha1,2-linked Gal residues in theN-linked chains. In
contradiction to many galactomannan-producing fungi, which add substantial
amounts of Gal in the furanose form to their glycoproteins, all detectable
Gal in the large S.pombe galactomannans is in the pyranose form, as found
in higher eukaryotes. The current work shows that the S.pombe outer chain
structure is a poly-alpha1,6Man backbone 2- O-substituted with either Gal
or the pyruvylated galactobiose and contains little alpha1,2-linked or
2-O-substituted Man. This is in contrast to the S. cerevisiae outer chain,
which is poly-alpha1,6Man substituted with alpha1,2-linked Man sidechains
(Ballou,C.E. ,1990, Methods Enzymol , 185, 440-470).
相似文献
99.
Plant-mediated soil legacy effects can be important determinants of the performance of plants and their aboveground insect herbivores, but, soil legacy effects on plant–insect interactions have been tested for only a limited number of host plant species and soils. Here, we tested the performance of a polyphagous aboveground herbivore, caterpillars of the cabbage moth Mamestra brassicae, on twelve host plant species that were grown on a set of soils conditioned by each of these twelve species. We tested how growth rate (fast- or slow-growing) and functional type (grass or forb) of the plant species that conditioned the soil and of the responding host plant species growing in those soils affect the response of insect herbivores to conditioned soils. Our results show that plants and insect herbivores had lower biomass in soils that were conditioned by fast-growing forbs than in soils conditioned by slow-growing forbs. In soils conditioned by grasses, growth rate of the conditioning plant had the opposite effect, i.e. plants and herbivores had higher biomass in soils conditioned by fast-growing grasses, than in soils conditioned by slow-growing grasses. We show that the response of aboveground insects to soil legacy effects is strongly positively correlated with the response of the host plant species, indicating that plant vigour may explain these relationships. We provide evidence that soil communities can play an important role in shaping plant–insect interactions aboveground. Our results further emphasize the important and interactive role of the conditioning and the response plant in mediating soil–plant–insect interactions. 相似文献
100.
Jér?me Beaufays Beno?t Adam Catherine Menten-Dedoyart Laurence Fievez Amélie Grosjean Yves Decrem Pierre-Paul Prév?t Sébastien Santini Robert Brasseur Michel Brossard Michel Vanhaeverbeek Fabrice Bureau Ernst Heinen Laurence Lins Luc Vanhamme Edmond Godfroid 《PloS one》2008,3(12)