Human follicular dendritic cells in vitro and follicular dendritic-cell-like cells

Cell and Tissue Research - Human follicular dendritic cell (FDC)-like cells (FLC) have been utilized for the in vitro analysis of germinal center reactions. However, there is no consensus whether...     

GYMNASTS UTILIZE VISUAL AND AUDITORY INFORMATION FOR BEHAVIOURAL SYNCHRONIZATION IN TRAMPOLINING

In synchronized trampolining, two gymnasts perform the same routine at the same time. While trained gymnasts are thought to coordinate their own movements with the movements of another gymnast by detecting relevant movement information, the question arises how visual and auditory information contribute to the emergence of synchronicity between both gymnasts. Therefore the aim of this study was to examine the role of visual and auditory information in the emergence of coordinated behaviour in synchronized trampolining. Twenty female gymnasts were asked to synchronize their leaps with the leaps of a model gymnast, while visual and auditory information was manipulated. The results revealed that gymnasts needed more leaps to reach synchronicity when only either auditory (12.9 leaps) or visual information (10.8 leaps) was available, as compared to when both auditory and visual information was available (8.1 leaps). It is concluded that visual and auditory information play significant roles in synchronized trampolining, whilst visual information seems to be the dominant source for emerging behavioural synchronization, and auditory information supports this emergence.     

Changes in enzyme stability and fatty acid composition of Streptomyces sp., a facultative thermophilic actinomycete

The thermostability of several enzymes from the facultative thermophilic actinomycete Streptomyces sp., derived from cells grown in the temperature range from 37 degrees C to 60 degrees C, has been examined. A correlation between the growth temperature of the cultures and the heat stability of the enzymes could be demonstrated for alanine dehydrogenase, isocitrate dehydrogenase, myokinase and pyruvate kinase. Except for the isocitrate dehydrogenase, which showed a linear increase of its stability throughout the entire temperature range, all enzymes exhibited a steep increase of the heat stability up to about 50 degrees C, but no further increase in the higher growth range, suggesting, that from 50 degrees C upward a shift to the exclusive production of thermostable protein occurs. Furthermore, the stability of alanine dehydrogenase and pyruvate kinase was found to increase substantially in presence of their substrates. In contrast, substrate-mediated stabilization was very weak with glucose-6-phosphate dehydrogenase and totally absent with acetate kinase, isocitrate dehydrogenase and myokinase. A comparison with previous observations with the same enzymes from Bacillus flavothermus showed, that enzymes from different organisms can have different thermal properties. Determination of the fatty acid composition of Streptomyces sp. cells, grown at different temperatures, showed relatively small alterations, with the main changes occurring between 37 degrees C and 40 degrees C.     

Peritoneal resorption of ethidium bromide, free or linked to DNA in rodents]

Ethidium bromide, either free (EB) or bound to DNA (EB-DNA), is injected into the peritoneal cavity of adult rats or mice. EB is then detected by fluorescence microscopy in peritoneal cells and by spectrophotometry in the peritoneal fluid. EB-DNA persists for a longer period of time in the peritoneal cavity than free EB does.     

Histophysiological modifications of the amnion in the rat at the end of gestation]

Amniotic epithelium of the rat has been studied during the eight last days of pregnancy, by scanning electron microscope analysis. The cells of this organ progressively become larger and flatter. Changes on their surface have also been observed : density of microvilli increases but single cilia disappear. These changes are related to modifications in the exchange processes.     

Bacillus flavothermus,a newly isolated facultative thermophile

A sample from a hot spring on the northern island of New Zealand contained five different thermophilic bacterial strains. One strain with peculiar properties, i.e. the formation of dark yellow colonies at 30°C as well as at 70°C, was further characterized. It was found to be a gram-positive, facultatively aerobic, motile Bacillus species, with terminal endospores. According to the physiological properties the strain closely resembled B. coagulans. However, two typical characteristics were contradictory to this conclusion, namely the intense yellow pigmentation of the colonies and the range of growth temperature. The latter was found to reach from 30 to 70°C, with an optimum at 60°C under aerobic and at 65°C under anaerobic conditions. Growth at moderate temperatures was slower than at 60°C, but the final cell yields were almost equal. The strain can therefore be considered as facultatively thermophilic. The pigment, which was found to be located in the cytoplasmic membrane, was spectroscopically identified as a carotenoid.Because the characteristics of this strain did not correspond with any of the Bacillus species described thus far, we concluded, that we had isolated a novel strain, for which the name Bacillus flavothermus is proposed.     

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