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Cultured neural crest cells which are freshly trypsinized require serum or purified fibronectin to attach to collagen substrates of types I–V. Furthermore, neural crest cells migrate in a Boyden chamber in response to fibronectin, and a “checkerboard” analysis demonstrates that fibronectin is both chemotactic and chemokinetic for these cells. It is proposed that collagen serves as a substrate for neural crest cells as they migrate in the early embryo. By mediating the cells' attachment to collagen, fibronectin may influence the movement of the cells. Local differences in fibronectin concentration or availability in the matrix could affect the degree of attachment of the cells to the collagen substrate and could also direct their migration by serving as a chemoattractant.  相似文献   
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Hydrobiologia - The deep mining of copper and nickel at Outokumpu, Eastern Finland, lasted from 1910 to the late 1980s, during which period metalliferous waste water of high conductivity and...  相似文献   
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The persent paper contains a study un the starch content of the chloroplasts of the leaves of Stellaria media, performed by hsitochemical methods. Three leaves were collected at short intervals, fixed and cut in 10 μ sections, and stained according to the PAS-procedure. The starch content of single chloroplasts was measured by a microscope photometer. The chemical composition of the stainable material was demonstrated by enzyme specific experiments. At the end of a 12 hours' dark period the chloroplasts contained only traces of starch. Light caused the starch accumulation to begin and after a time chloroplasts seemed to be filled with starch. Subsequently, however, a sudden decrease in the starch content of the chloroplasts took place, in spite of constant illumination. A rather high level of starch content was restored in a few hours. The author puts forward some ideas about the nature of the factors causing the transitory disintegration of starch in illuminated leaves. The induction or the activation of certain enzymes seems to be the most probable explanalion.  相似文献   
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Aim  We aim to assess the impact of forest fragmentation on lepidopteran larval community and study the associations of microclimate and tree community with lepidopteran assemblage.
Location  Kibale National Park, Uganda.
Methods  We investigated the effects of forest fragmentation on leaf herbivory, density of lepidopteran caterpillars, species richness and diversity as well as the composition of herbivorous lepidopteran larval community. Microclimate, size of the fragment, distance to the continuous forest, and tree diversity were studied as possible explanatory factors. We sampled 10 Neoboutonia macrocalyx Pax. (Euphorbiaceae) trees in each fragment during dry and rainy season, total of four times, in a year to cover the seasonal variation.
Results  The rates of herbivory, total larval density and species richness were significantly lower in the forest fragments than in the continuous forest but species diversity expressed as Fisher's alpha did not differ. The dominance structure and community composition of the larval communities in the fragments was different from that of the continuous forest. None of the differences we observed were related to the fragment area or distance to the continuous forest. Instead, we found an indication of association between the herbivore and the tree communities. The fragments had significantly lower humidity during most of the day and higher temperature during the afternoons (14–17 h), which might partially explain the differences in lepidopteran larval communities.
Main conclusions  Decreased larval density and species richness as well as differences in the community composition and structure all highlight the importance of large continuous forest areas for maintaining larval biodiversity.  相似文献   
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Tree seasonality in a warming climate   总被引:1,自引:0,他引:1  
Climate warming has increased researchers' interest in plant phenology and its modelling. Although the main focus is on projections of accelerated springtime phenological events, also a further extension of the growing season by delayed growth cessation is often projected. However, ecophysiological studies indicate that, for boreal and temperate trees, such generalisations are precluded owing to differential climatic conditions and inter- and intraspecific genetic differences. The annual cycle of these trees is an integrated system, where one phase affects subsequent phases, resulting in delayed impacts, which are only partially addressed in current ecophysiological models. Here, we outline an updated integrated conceptual model of the annual cycle by identifying ecophysiological phenomena that are particularly significant under climate warming.  相似文献   
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Peroxisome proliferator-activated receptor (PPAR) delta is an important regulator of fatty acid (FA) metabolism. Angiopoietin-like 4 (Angptl4), a multifunctional protein, is one of the major targets of PPAR delta in skeletal muscle cells. Here we investigated the regulation of Angptl4 and its role in mediating PPAR delta functions using human, rat and mouse myotubes. Expression of Angptl4 was upregulated during myotubes differentiation and by oleic acid, insulin and PPAR delta agonist GW501516. Treatment with GW501516 or Angptl4 overexpression inhibited both lipoprotein lipase (LPL) activity and LPL-dependent uptake of FAs whereas uptake of BSA-bound FAs was not affected by either treatment. Activation of retinoic X receptor (RXR), PPAR delta functional partner, using bexarotene upregulated Angptl4 expression and inhibited LPL activity in a PPAR delta dependent fashion. Silencing of Angptl4 blocked the effect of GW501516 and bexarotene on LPL activity. Treatment with GW501516 but not Angptl4 overexpression significantly increased palmitate oxidation. Furthermore, Angptl4 overexpression did not affect the capacity of GW501516 to increase palmitate oxidation. Basal and insulin stimulated glucose uptake, glycogen synthesis and glucose oxidation were not significantly modulated by Angptl4 overexpression. Our findings suggest that FAs-PPARdelta/RXR-Angptl4 axis controls the LPL-dependent uptake of FAs in myotubes, whereas the effect of PPAR delta activation on beta-oxidation is independent of Angptl4.  相似文献   
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The effects of electromagnetic fields (EMF) emitted by cellular phones on the event related desynchronization/synchronization (ERD/ERS) of the 4-6, 6-8, 8-10, and 10-12 Hz electroencephalogram (EEG) frequency bands were studied in 24 normal subjects performing an auditory memory task. This study was a systematic replication of our previous work. In the present double blind study, all subjects performed the memory task both with and without exposure to a digital 902 MHz field in a counterbalanced order. We were not able to replicate the findings from our earlier study. All eight of the significant changes in our earlier study were not significant in the present double blind replication. Also, the effect of EMF on the number of incorrect answers in the memory task was inconsistent. We previously reported no significant effect of EMF exposure on the number of incorrect answers in the memory task, but a significant increase in errors was observed in the present study. We conclude that EMF effects on the EEG and on the performance on memory tasks may be variable and not easily replicable for unknown reasons.  相似文献   
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