Phylogeny of Syrphidae (Diptera) inferred from combined analysis of molecular and morphological characters

Abstract.  Syrphidae (Diptera) commonly called hoverflies, includes more than 5000 species world-wide. The aim of this study was to address the systematic position of the disputed elements in the intrafamilial classification of Syrphidae, namely the monophyly of Eristalinae and the placement of Microdontini and Pipizini, as well as the position of particular genera ( Nausigaster , Alipumilio , Spheginobaccha ). Sequence data from nuclear 28S rRNA and mitochondrial COI genes in conjunction with larval and adult morphological characters of fifty-one syrphid taxa were analysed using optimization alignment to explore phylogenetic relationships among included taxa. A species of Platypezidae, Agathomyia unicolor , was used as outgroup, and also including one representative ( Jassidophaga villosa ) of the sister-group of Syrphidae, Pipunculidae. Sensitivity of the data was assessed under six different parameter values. A stability tree summarized the results. Microdontini, including Spheginobaccha , was placed basally, and Pipizini appeared as the sister-group to subfamily Syrphinae. The monophyly of subfamily Eristalinae was supported. The results support at least two independent origins of entomophagy in syrphids, and frequent shifts between larval feeding habitats within the saprophagous eristalines.     

Interpopulation genetic divergence in European grayling (Thymallus thymallus, Salmonidae) at a microgeographic scale: implications for conservation

In order to provide guidelines for conservationand management of the severely declining LakeSaimaa (eastern Finland) European grayling(Thymallus thymallus, Salmonidae), weinvestigated the microgeographic geneticstructure of three populations from the watersystem using 17 microsatellite loci andmitochondrial DNA polymerase chainreaction-restriction fragment lengthpolymorphism analysis. Microsatellites revealedlow levels of intrapopulation genetic diversityand substantial divergence between populationssampled from spawning sites separated by aslittle as 55 kilometers. Mitochondrial analysisindicated the occurrence of two compositehaplotypes within Lake Saimaa. The nucleotidesubstitution estimates between the haplotypessuggested their separation to markedly predatethe late Pleistocene period. The populationsexhibited marked frequency differences for thetwo mitochondrial haplotypes, reinforcing theview of limited interpopulation gene flowwithin Lake Saimaa. An individual basedmicrosatellite Neighbor-Joining dendrogramdemonstrated clustering of all the specimensaccording to their sampling origin. Individualassignment tests revealed 100% assignmentsuccess of individuals into their population oforigin and 100% exclusion (p <0.05) from all alternative referencepopulations. These findings exemplify that T. thymallus populations may be genetically highly structured over small geographical scales and provide a good starting point for the development of appropriate conservation strategies for Lake Saimaa grayling.     

Effects of Metarhizium anisopliae on the pollen beetle Meligethes aeneus and its parasitoids Phradis morionellus and Diospilus capito

The efficacy of two Finnish strains of Metarhizium anisopliaeagainst the rape blossom beetle Meligethes aeneus (Coleoptera:Nitidulidae) and the effect on its parasitoids Phradismorionellus (Hymenoptera: Ichneumonidae) and Diospilus capito(Hymenoptera: Braconidae) were studied in laboratory and semi-fieldexperiments. Several bio-assays were performed using either directexposure of the host beetle to the pathogens, or pots containing soilinoculated with M. anisopliae conidia at a rate of 2 ×10⁸ per ml soil. Parasitised and unparasitised M. aeneuslarvae were collected in the field and were placed for pupation in thetest soil. The effect of treatment on M. aeneus andD. capito was estimated after adult emergence, and the effecton P. morionellus was estimated by dissecting hibernating pupae.While both of the M. anisopliae strains were highly pathogenicto M. aeneus adults and larvae upon direct exposure, soiltreatment resulted in no significant difference between the number ofinsects emerging (or found) from the treated and untreatedpots. However, the treated insects appeared to be latently infectedand the actual infection rate had to be estimated after their deathand incubation in a moist chamber. The mortality of the beetles causedby direct exposure to the fungus was 85% (range 70–88%). The rateof latent infection following indirect exposure via treated soil indeep pots in laboratory was 80% (range 49–100%), while that inP. morionellus was only 17% (range 0–85%), and inD. capito significantly higher, 76% (range 72–100%).The results indicate that M. anisopliae is a potentially usefulcandidate to be used as a bio-insecticide to control the pollenbeetle, and that at least the most abundant parasitoid, Phradismorionellus, is less affected than the target pest itself. Thepossible ecological role of latent infections in the host as well asin some of the parasitoids needs to be clarified.     

Unraveling the metabolic underpinnings of frailty using multicohort observational and Mendelian randomization analyses

Identifying metabolic biomarkers of frailty, an age-related state of physiological decline, is important for understanding its metabolic underpinnings and developing preventive strategies. Here, we systematically examined 168 nuclear magnetic resonance-based metabolomic biomarkers and 32 clinical biomarkers for their associations with frailty. In up to 90,573 UK Biobank participants, we identified 59 biomarkers robustly and independently associated with the frailty index (FI). Of these, 34 associations were replicated in the Swedish TwinGene study (n = 11,025) and the Finnish Health 2000 Survey (n = 6073). Using two-sample Mendelian randomization, we showed that the genetically predicted level of glycoprotein acetyls, an inflammatory marker, was statistically significantly associated with an increased FI (β per SD increase = 0.37%, 95% confidence interval: 0.12–0.61). Creatinine and several lipoprotein lipids were also associated with increased FI, yet their effects were mostly driven by kidney and cardiometabolic diseases, respectively. Our findings provide new insights into the causal effects of metabolites on frailty and highlight the role of chronic inflammation underlying frailty development.     

Trophic interactions in changing landscapes: responses of soil food webs

Soil communities in landscapes that are rapidly changing due to a range of anthropogenic processes can be regarded as highly transient systems where interactions between competing species or trophic levels may be seriously disrupted. In disturbed communities dispersal in space and time has a role in ensuring continuity of community function. Stable communities, in undisturbed systems, are more dependent on competition and other biotic interactions between species. We predicted how food web components would respond to disturbance, based on their dispersal and colonizing abilities. During decomposition, flows of energy and nutrients generally follow either a bacterial-based path, with bacteria as the primary decomposer and bacterial-feeding fauna and their predators forming the associated food web, or a fungal-based channel. Trophic links that were generally resistant to change were the organisms of the bacterial pathway that have high abilities to disperse in time and passively disperse in space. Organisms in the fungal pathway were less resistant to disturbance. Resource inputs to the soil system are derived from plants, either through root exudation and root turnover during active growth or from dead plant material following senescence or agricultural tillage. Disturbances to the soil system can arise as a direct action on the soil, or indirectly from effects on the above-ground plant community. Disturbance-induced changes in plant community composition will change the soil food web composition. Organisms involved in direct interactions with plants (e.g. AM-mycorrhizal fungi) were also predicted to be vulnerable to disturbance.

Zusammenfassung

Bodengemeinschaften in Landschaften, die sich aufgrund einer Reihe von anthropogenen Prozessen schnellstens verändern, können als sehr kurzlebige Systeme angesehen werden, in denen Interaktionen zwischen konkurrierenden Arten oder trophischen Leveln nachhaltig unterbrochen sind. In gestörten Gemeinschaften hat die Ausbreitung in Raum und Zeit eine Rolle bei der Wahrung der Kontinuität von Gemeinschaftsfunktionen. Stabile Gemeinschaften, in ungestörten Systemen, sind stärker von Konkurrenz und anderen biotischen Interaktionen zwischen den Arten abhängig. Wir sagten voraus, wie Nahrungsnetzkomponenten auf Störung antworten würden, basierend auf ihrer Ausbreitungs- und Kolonisationsfähigkeit. Während der Zersetzung folgen die Flüsse von Energie und Nährstoffen im Allgemeinen entweder einem Weg, der auf Bakterien basiert, mit Bakterien als den primären Zersetzern und bacterienfressender Fauna und ihre Predatoren, die das assoziierte Nahrungsnetz bilden, oder sie folgen einem Kanal, der auf Pilzen basiert. Trophische Verknüpfungen, die im Allgemeinen resistent gegen Veränderungen waren, waren die Organismen des bakteriellen Weges, die große Möglichkeiten haben sich in Zeit und passiv im Raum auszubreiten. Organismen des pilzbasierten Weges waren weniger widerstandsfähig gegenüber Störungen. Die Ressourcenzufuhr in das Bodensystem stammte von Pflanzen, entweder über Wurzelausscheidungen und/oder Wurzelturnover während des aktiven Wachstums oder von totem Pflanzenmaterial aufgrund von Seneszenz oder landwirtschatlicher Bodenbearbeitung. Störungen des Bodensystems können durch direkte Einwirkungen auf den Boden oder indirekt durch Effekte der oberirdischen Pflanzemeinschaft entstehen. Störungsinduzierte Veränderungen in der Zusammensetzung der Pflanzengemeinschaft werden die Zusammensetzung des Bodennahrungsnetzes verändern. Für Organismen, die an direkten Interaktionen mit Pflanzen beteiligt sind (beispielsweise AM-Mykorrhizapilze), wurde ebenfalls vorhergesagt, dass sie anfällig für Störungen sind.