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Sedimentation of phytoplankton was studied in the meso/eutrophic reservoir Saidenbach for two years and measured as biovolume in a sedimentation trap near the bottom. It comes to an annual average of 2.76cm3/m2 × d (0.4… 10.9) and is statistically significant dependent on the free water concentration measured 14 days before. This allows flux to be reliably calculated without any direct measurement. The bottom is reached above all by diatoms which form 90% of the deposited algae. The sinking velocity of the diatoms is mainly determined by their physiological state: During growth phases low sinking velocities (0.1 … 2m/d) were found, while in decline phases they increased considerably (>6m/d). The highest average sinking velocities found among large diatoms were calculated for Fragilaria crotonensis (3…4m/d), the lowest for Melosira italica (1.5…2m/d). The values for Asterionella formosa, Synedra acus and Diatoma elongatum were between them. Turbulence has no influence on sinking velocity (usually, spring full circulation sees the highest sedimentary losses), but plays an essential part in the survival of the population in free water. Permanent redistribution prevents diatoms from sinking out from the euphotic layer, this “inoculation” making further development possible. On an annual average, phytoplankton forms approximately only one quarter of the whole trap sediment (max. 62%). The flux of the dry weight of seston (2.33g/m2 x d on an average) reflects the changes in phytoplankton flux negligibly only and does not reveal any relation to it. so that seston flux is not suitable for determining phytoplankton sedimentation. But it is possible to calculate this at a probability of 65 to 94%, by using the free water concentration measured 14 days before. 相似文献
424.
A retention model for the chiral separation of an uncharged solute, felodipine, on CHIRAL-AGP, using a micellar mobile phase is proposed. The model assumes the presence of two stereoselective sites and each enantiomer was found to interact with different sites. Addition of a chiral aliphatic alcohol, (+)-(S)-2-octanol, preferentially interacted with the binding site for (?)-(S)-felodipine. The monomeric form of the micellar agent (Tween® 20) competed with the enantiomers for the adsorption sites, and the formation of a 1:1 complex between the enantiomers and the micelles was assumed. The retention of the solutes was effectively controlled by adding small quantities (<1.63 × 10?3 M) of the nonionic detergent Tween 20 to the mobile phase. Baseline separation was achieved by addition of 1.0 mM n-octylamine to the mobile phase; 8.14 × 10?4 M Tween 20 in phosphate buffer pH 7.0. The separation factor (α = 1.74) was unaffected by the detergent concentration in the presence of 1.0 mM n-octylamine. © 1995 Wiley-Liss, Inc. 相似文献