全文获取类型
收费全文 | 1214篇 |
免费 | 71篇 |
国内免费 | 1篇 |
出版年
2024年 | 2篇 |
2023年 | 2篇 |
2022年 | 11篇 |
2021年 | 25篇 |
2020年 | 15篇 |
2019年 | 16篇 |
2018年 | 35篇 |
2017年 | 23篇 |
2016年 | 35篇 |
2015年 | 61篇 |
2014年 | 82篇 |
2013年 | 83篇 |
2012年 | 91篇 |
2011年 | 89篇 |
2010年 | 48篇 |
2009年 | 61篇 |
2008年 | 72篇 |
2007年 | 89篇 |
2006年 | 47篇 |
2005年 | 55篇 |
2004年 | 55篇 |
2003年 | 49篇 |
2002年 | 37篇 |
2001年 | 28篇 |
2000年 | 31篇 |
1999年 | 22篇 |
1997年 | 3篇 |
1996年 | 4篇 |
1995年 | 5篇 |
1993年 | 2篇 |
1992年 | 11篇 |
1991年 | 6篇 |
1990年 | 9篇 |
1989年 | 16篇 |
1988年 | 5篇 |
1987年 | 10篇 |
1986年 | 7篇 |
1985年 | 4篇 |
1984年 | 7篇 |
1983年 | 5篇 |
1982年 | 5篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1978年 | 2篇 |
1976年 | 3篇 |
1973年 | 2篇 |
1971年 | 3篇 |
1970年 | 1篇 |
1965年 | 2篇 |
1934年 | 1篇 |
排序方式: 共有1286条查询结果,搜索用时 46 毫秒
101.
102.
Xi Cheng Sunhwan Jo Yifei Qi Francesca?M. Marassi Wonpil Im 《Biophysical journal》2015,108(8):1954-1962
Solid-state NMR has been used to determine the structures of membrane proteins in native-like lipid bilayer environments. Most structure calculations based on solid-state NMR observables are performed using simulated annealing with restrained molecular dynamics and an energy function, where all nonbonded interactions are represented by a single, purely repulsive term with no contributions from van der Waals attractive, electrostatic, or solvation energy. To our knowledge, this is the first application of an ensemble dynamics technique performed in explicit membranes that uses experimental solid-state NMR observables to obtain the refined structure of a membrane protein together with information about its dynamics and its interactions with lipids. Using the membrane-bound form of the fd coat protein as a model membrane protein and its experimental solid-state NMR data, we performed restrained ensemble dynamics simulations with different ensemble sizes in explicit membranes. For comparison, a molecular dynamics simulation of fd coat protein was also performed without any restraints. The average orientation of each protein helix is similar to a structure determined by traditional single-conformer approaches. However, their variations are limited in the resulting ensemble of structures with one or two replicas, as they are under the strong influence of solid-state NMR restraints. Although highly consistent with all solid-state NMR observables, the ensembles of more than two replicas show larger orientational variations similar to those observed in the molecular dynamics simulation without restraints. In particular, in these explicit membrane simulations, Lys40, residing at the C-terminal side of the transmembrane helix, is observed to cause local membrane curvature. Therefore, compared to traditional single-conformer approaches in implicit environments, solid-state NMR restrained ensemble simulations in explicit membranes readily characterize not only protein dynamics but also protein-lipid interactions in detail. 相似文献
103.
Yifei Qi Xi Cheng Jumin Lee Josh?V. Vermaas Taras?V. Pogorelov Emad Tajkhorshid Soohyung Park Jeffery?B. Klauda Wonpil Im 《Biophysical journal》2015,109(10):2012-2022
Slow diffusion of the lipids in conventional all-atom simulations of membrane systems makes it difficult to sample large rearrangements of lipids and protein-lipid interactions. Recently, Tajkhorshid and co-workers developed the highly mobile membrane-mimetic (HMMM) model with accelerated lipid motion by replacing the lipid tails with small organic molecules. The HMMM model provides accelerated lipid diffusion by one to two orders of magnitude, and is particularly useful in studying membrane-protein associations. However, building an HMMM simulation system is not easy, as it requires sophisticated treatment of the lipid tails. In this study, we have developed CHARMM-GUI HMMM Builder (http://www.charmm-gui.org/input/hmmm) to provide users with ready-to-go input files for simulating HMMM membrane systems with/without proteins. Various lipid-only and protein-lipid systems are simulated to validate the qualities of the systems generated by HMMM Builder with focus on the basic properties and advantages of the HMMM model. HMMM Builder supports all lipid types available in CHARMM-GUI and also provides a module to convert back and forth between an HMMM membrane and a full-length membrane. We expect HMMM Builder to be a useful tool in studying membrane systems with enhanced lipid diffusion. 相似文献
104.
Hyun-Lim Kim Hana Ra Ki-Ryeong Kim Jeong-Min Lee Hana Im Yang-Hee Kim 《Molecules and cells》2015,38(4):312-317
Depletion of intracellular zinc by N,N,N′,N′-tetrakis(2-pyridylmethyl) ethylenediamine (TPEN) induces p53-mediated protein synthesis-dependent apoptosis of mouse cortical neurons. Here, we examined the requirement for poly(ADP-ribose) polymerase (PARP)-1 as an upstream regulator of p53 in zinc depletion-induced neuronal apoptosis. First, we found that chemical inhibition or genetic deletion of PARP-1 markedly attenuated TPEN-induced apoptosis of cultured mouse cortical neurons. Poly(ADP-ribosyl)ation of p53 occurred starting 1 h after TPEN treatment. Suggesting the critical role of PARP-1, the TPEN-induced increase of stability and activity of p53 as well as poly(ADP-ribosyl)ation of p53 was almost completely blocked by PARP inhibition. Consistent with this, the induction of downstream proapoptotic proteins PUMA and NOXA was noticeably reduced by chemical inhibitors or genetic deletion of PARP-1. TPEN-induced cytochrome C release into the cytosol and caspase-3 activation were also blocked by inhibition of PARP-1. Taken together, these findings indicate that PARP-1 is essential for TPEN-induced neuronal apoptosis. 相似文献
105.
106.
107.
108.
109.
Paik SH Kim HJ Lee S Im SW Ju YS Yeon JH Jo SJ Eun HC Seo JS Kim JI Kwon OS 《BMB reports》2011,44(11):741-746
Tanning ability is important, because it represents the ability of the skin to protect itself against ultraviolet (UV) radiation. Here, we sought to determine genetic regions associated with tanning ability. Skin pigmentation was measured at the outer forearm and buttock areas to represent facultative and constitutive skin color, respectively. In our study population consisting of isolated Mongolian subjects, with common histories of environmental UV exposure during their nomadic life, facultative skin color adjusted by constitutive skin color was used to indicate tanning ability. Through linkage analysis and family-based association tests of 345 Mongolian subjects, we identified 2 potential linkage regions regulating tanning ability on 5q35.3 and 12q13.2, having 6 and 7 significant single nucleotide polymorphisms (SNPs), respectively. Those significant SNPs were located in or adjacent to potential candidate genes related to tanning ability: GRM6, ATF1, WNT1, and SILV/Pmel17. 相似文献
110.