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991.
Richard Appeldoorn David Ballantine Ivonne Bejarano Milton Carlo Michael Nemeth Ernesto Otero Francisco Pagan Hector Ruiz Nikolaos Schizas Clark Sherman Ernesto Weil 《Coral reefs (Online)》2016,35(1):63-75
Mesophotic coral ecosystems (MCEs) were compared between La Parguera and Ponce, off the south coast of Puerto Rico. In contrast to La Parguera, Ponce has a narrow insular shelf and hosts several river outlets, a commercial port, a regional sewage treatment plant with associated deep water outfall, and three deep dredge disposal sites. Off Ponce, MCEs receive higher (16×) rates of sedimentation than off La Parguera, a less impacted site. The most impacted sites were located offshore of Cayo Ratones and are in or down-current and in close proximity to one of the dredge disposal sites. There, MCEs are characterized by a steep, irregular, rocky slope with a cover of fine-grained, dark brown sediment, which increases with depth. At shallower depths, scattered rocky outcroppings are colonized by sponges, black corals and algae. The sediment cover contains two to three times the terrigenous content and a significantly higher percentage of the fine-grained fraction than off La Parguera. Thirteen remotely operated vehicle (ROV) dives east and west of Ponce showed that the deepest depth at which corals were observed increased with distance from Cayo Ratones and did not approach depths observed off La Parguera except at the eastern-most (up-current) site, Caja de Muertos, which was also significantly further offshore. Benthic communities off Caja de Muertos were comparable to those at La Parguera, while off Cayo Ratones, there were no mesophotic corals and sparse development of other benthic macrobiota except sponges. Management authorities should include MCEs when assessing potential impacts from anthropogenic activities and take the necessary steps to reduce local threats. 相似文献
992.
993.
Yulheri Abas Nadia Touil Micheline Kirsch-Volders Geert Angenon Michel Jacobs Ivan Diane Hector Famelaer 《Plant Cell, Tissue and Organ Culture》2007,91(2):145-154
The aim of the present study was to observe the induction and repair of single strand breaks (Ssbs) and double strand breaks
(Dsbs) in mesophyll protoplasts of Nicotiana plumbaginifolia, irradiated with UV-C and cultured under light or dark conditions. DNA damage and repair was determined by the neutral and
alkaline comet assay to reveal Dsbs and Ssbs respectively.
Subculturing protoplasts for 4 h at low temperature was essential to reduce the amount of Dsbs to the detection limit of the
assay procedure.
Light-cultured protoplasts showed a significant increase of Ssbs and Dsbs compared to dark cultured protoplasts, in which
the number of Ssbs and Dsbs remained very constant throughout the experiments.
UV treatment significantly enhanced the levels of Ssbs and Dsbs in light and dark cultured protoplasts. On average, equal
levels of DNA damage were observed under light or dark conditions. Formulations introduced to evaluate the contribution of
UV-C or light treatment in repair kinetics of DNA damage, showed that the number of Ssbs, but not of Dsbs, evolved differently
for light and dark cultured protoplasts. DNA repair was more rapidly observed under light conditions and occurred in different
repair phases. Observations are discussed in relation to the involvement of chromatin remodelling, photosynthetic active radiation
and DNA repair mechanisms. 相似文献
994.
995.
As a result of global climate change, species are experiencing an escalation in the severity and regularity of extreme thermal events. With patterns of disease distribution and transmission predicted to undergo considerable shifts in the coming years, the interplay between temperature and pathogen exposure will likely determine the capacity of a population to persist under the dual threat of global change and infectious disease. In this study, we investigated how exposure to a pathogen affects an individual's ability to cope with extreme temperatures. Using experimental infections of Daphnia magna with its obligate bacterial pathogen Pasteuria ramosa, we measured upper thermal limits of multiple host and pathogen genotype combinations across the dynamic process of infection and under various forms (static and ramping) of thermal stress. We find that pathogens substantially limit the thermal tolerance of their host, with the reduction in upper thermal limits on par with the breadth of variation seen across similar species entire geographical ranges. The precise magnitude of any reduction, however, was specific to the host and pathogen genotype combination. In addition, as thermal ramping rate slowed, upper thermal limits of both healthy and infected individuals were reduced. Our results suggest that the capacity of a population to evolve new thermal limits, when also faced with the threat of infection, will depend not only on a host's genetic variability in warmer environments, but also on the frequency of host and pathogen genotypes. We suggest that pathogen‐induced alterations of host thermal performance should be taken into account when assessing the resilience of any population and its potential for adaptation to global change. 相似文献
996.
997.
An X-ray microanalysis survey of the concentration of elements in the cytoplasm of different mammalian cell types 总被引:1,自引:0,他引:1
Electron probe energy dispersive X-ray microanalysis was performed on freeze-dried tissue sections. The dry weight concentration of elements (mmole/kg dry weight) was measured in the cytoplasm of several cell types from adult mice and rats. This comparative investigation showed: (1) That the energy dispersive X-ray spectrum of element concentration from the cytoplasm of a specific cell type allows one to distinguish this specific cell type from other cell types with considerable accuracy. (2) That there is a relationship between the concentration of the various elements and the ultrastructural features of the cytoplasmic regions being analyzed. For example, areas rich in ribosomes are also rich in P, K and Mg. (3) These data support the idea that K is directly involved in the control of protein synthesis. The catalog of element concentrations in the cytoplasm of 13 cell types from both mice and rats should be of value to others who seek to answer various questions about these cell types. 相似文献
998.
999.