全文获取类型
收费全文 | 4743篇 |
免费 | 443篇 |
国内免费 | 2篇 |
出版年
2023年 | 15篇 |
2022年 | 44篇 |
2021年 | 119篇 |
2020年 | 72篇 |
2019年 | 86篇 |
2018年 | 80篇 |
2017年 | 77篇 |
2016年 | 155篇 |
2015年 | 239篇 |
2014年 | 251篇 |
2013年 | 285篇 |
2012年 | 417篇 |
2011年 | 421篇 |
2010年 | 257篇 |
2009年 | 194篇 |
2008年 | 326篇 |
2007年 | 348篇 |
2006年 | 266篇 |
2005年 | 300篇 |
2004年 | 266篇 |
2003年 | 228篇 |
2002年 | 234篇 |
2001年 | 34篇 |
2000年 | 44篇 |
1999年 | 54篇 |
1998年 | 60篇 |
1997年 | 34篇 |
1996年 | 30篇 |
1995年 | 28篇 |
1994年 | 18篇 |
1993年 | 12篇 |
1992年 | 11篇 |
1991年 | 13篇 |
1990年 | 13篇 |
1989年 | 12篇 |
1987年 | 8篇 |
1986年 | 8篇 |
1985年 | 5篇 |
1984年 | 7篇 |
1983年 | 12篇 |
1982年 | 9篇 |
1981年 | 8篇 |
1980年 | 8篇 |
1979年 | 6篇 |
1978年 | 8篇 |
1977年 | 8篇 |
1974年 | 9篇 |
1973年 | 4篇 |
1972年 | 4篇 |
1965年 | 4篇 |
排序方式: 共有5188条查询结果,搜索用时 531 毫秒
151.
Genome sequence of Babesia bovis and comparative analysis of apicomplexan hemoprotozoa 总被引:1,自引:0,他引:1
Brayton KA Lau AO Herndon DR Hannick L Kappmeyer LS Berens SJ Bidwell SL Brown WC Crabtree J Fadrosh D Feldblum T Forberger HA Haas BJ Howell JM Khouri H Koo H Mann DJ Norimine J Paulsen IT Radune D Ren Q Smith RK Suarez CE White O Wortman JR Knowles DP McElwain TF Nene VM 《PLoS pathogens》2007,3(10):1401-1413
152.
Y chromosome lineage- and village-specific genes on chromosomes 1p22 and 6q27 control visceral leishmaniasis in Sudan
下载免费PDF全文
![点击此处可从《PLoS genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Miller EN Fadl M Mohamed HS Elzein A Jamieson SE Cordell HJ Peacock CS Fakiola M Raju M Khalil EA Elhassan A Musa AM Ibrahim ME Blackwell JM 《PLoS genetics》2007,3(5):e71
Familial clustering and ethnic differences suggest that visceral leishmaniasis caused by Leishmania donovani is under genetic control. A recent genome scan provided evidence for a major susceptibility gene on Chromosome 22q12 in the Aringa ethnic group in Sudan. We now report a genome-wide scan using 69 families with 173 affected relatives from two villages occupied by the related Masalit ethnic group. A primary ten-centimorgan scan followed by refined mapping provided evidence for major loci at 1p22 (LOD score 5.65; nominal p = 1.72 × 10−7; empirical p < 1 × 10−5; λS = 5.1) and 6q27 (LOD score 3.74; nominal p = 1.68 × 10−5; empirical p < 1 × 10−4; λS = 2.3) that were Y chromosome–lineage and village-specific. Neither village supported a visceral leishmaniasis susceptibility gene on 22q12. The results suggest strong lineage-specific genes due to founder effect and consanguinity in these recently immigrant populations. These chance events in ethnically uniform African populations provide a powerful resource in the search for genes and mechanisms that regulate this complex disease. 相似文献
153.
Use of bimolecular fluorescence complementation to study in vivo interactions between Cdc42p and Rdi1p of Saccharomyces cerevisiae
下载免费PDF全文
![点击此处可从《Eukaryotic cell》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Saccharomyces cerevisiae Cdc42p functions as a GTPase molecular switch, activating multiple signaling pathways required to regulate cell cycle progression and the actin cytoskeleton. Regulatory proteins control its GTP binding and hydrolysis and its subcellular localization, ensuring that Cdc42p is appropriately activated and localized at sites of polarized growth during the cell cycle. One of these, the Rdi1p guanine nucleotide dissociation inhibitor, negatively regulates Cdc42p by extracting it from cellular membranes. In this study, the technique of bimolecular fluorescence complementation (BiFC) was used to study the dynamic in vivo interactions between Cdc42p and Rdi1p. The BiFC data indicated that Cdc42p and Rdi1p interacted in the cytoplasm and around the periphery of the cell at the plasma membrane and that this interaction was enhanced at sites of polarized cell growth during the cell cycle, i.e., incipient bud sites, tips and sides of small- and medium-sized buds, and the mother-bud neck region. In addition, a ring-like structure containing the Cdc42p-Rdi1p complex transiently appeared following release from G1-phase cell cycle arrest. A homology model of the Cdc42p-Rdi1p complex was used to introduce mutations that were predicted to affect complex formation. These mutations resulted in altered BiFC interactions, restricting the complex exclusively to either the plasma membrane or the cytoplasm. Data from these studies have facilitated the temporal and spatial modeling of Rdi1p-dependent extraction of Cdc42p from the plasma membrane during the cell cycle. 相似文献
154.
Functional modulation of cardiac form through regionally confined cell shape changes 总被引:1,自引:1,他引:0
下载免费PDF全文
![点击此处可从《PLoS biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Developing organs acquire a specific three-dimensional form that ensures their normal function. Cardiac function, for example, depends upon properly shaped chambers that emerge from a primitive heart tube. The cellular mechanisms that control chamber shape are not yet understood. Here, we demonstrate that chamber morphology develops via changes in cell morphology, and we determine key regulatory influences on this process. Focusing on the development of the ventricular chamber in zebrafish, we show that cardiomyocyte cell shape changes underlie the formation of characteristic chamber curvatures. In particular, cardiomyocyte elongation occurs within a confined area that forms the ventricular outer curvature. Because cardiac contractility and blood flow begin before chambers emerge, cardiac function has the potential to influence chamber curvature formation. Employing zebrafish mutants with functional deficiencies, we find that blood flow and contractility independently regulate cell shape changes in the emerging ventricle. Reduction of circulation limits the extent of cardiomyocyte elongation; in contrast, disruption of sarcomere formation releases limitations on cardiomyocyte dimensions. Thus, the acquisition of normal cardiomyocyte morphology requires a balance between extrinsic and intrinsic physical forces. Together, these data establish regionally confined cell shape change as a cellular mechanism for chamber emergence and as a link in the relationship between form and function during organ morphogenesis. 相似文献
155.
156.
Shender Lisa A. Cody Theresa Ruder Mark Fenton Heather Niedringhaus Kevin D. Blanton Jason Motes Jessy Schmedes Sarah Forys Elizabeth 《EcoHealth》2022,19(2):203-215
EcoHealth - Extreme weather events, particularly heavy rainfall, are occurring at greater frequency with climate change. Although adverse human health effects from heavy rainfall are often... 相似文献
157.
Katherine L. Helbig Robert J. Lauerer Jacqueline C. Bahr Ivana A. Souza Candace T. Myers Betül Uysal Niklas Schwarz Maria A. Gandini Sun Huang Boris Keren Cyril Mignot Alexandra Afenjar Thierry Billette de Villemeur Delphine Héron Caroline Nava Stéphanie Valence Julien Buratti Christina R. Fagerberg Heather C. Mefford 《American journal of human genetics》2019,104(3):562
158.
Amino Acids - It is known for almost 25 years that the corpora cardiaca (neurosecretory glands) of cicadas synthesize two isobaric peptides with hypertrehalosaemic activity denominated... 相似文献
159.
160.
Wenjuan Dong Heather Mead Lei Tian Jun-Gyu Park Juan I. Garcia Sierra Jaramillo Tasha Barr Daniel S. Kollath Vanessa K. Coyne Nathan E. Stone Ashley Jones Jianying Zhang Aimin Li Li-Shu Wang Martha Milanes-Yearsley Jordi B. Torrelles Luis Martinez-Sobrido Paul S. Keim Bridget Marie Barker Michael A. Caligiuri Jianhua Yu 《Journal of virology》2022,96(1)