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131.
C. W. Healey 《BMJ (Clinical research ed.)》1935,2(3898):558-559
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Matthew R. Steensma Wakenda K. Tyler Allison G. Shaber Steven R. Goldring F. Patrick Ross Bart O. Williams John H. Healey P. Edward Purdue 《PloS one》2013,8(7)
Giant cell tumor of bone (GCTB) is a benign, locally destructive neoplasm, with tumors comprised of mesenchymal fibroblast-like stromal cells; monocytic, mononuclear cells of myeloid lineage; and the characteristic osteoclast-like, multinucleated giant cells. Hampering the study of the complex interaction of its constituent cell types is the propensity of longstanding, repeatedly passaged cell cultures to undergo phenotypic alteration and loss of osteoclast-inducing capacities. In this study, we employed a novel, single-step technique to purify freshly harvested stromal cells using a CD14-negative selection column. Using 9 freshly harvested GCTB specimens and the purified stromal cell component, we performed analyses for markers of osteoblast lineage and analyzed the capacity of the stromal cells to undergo osteoblastic differentiation and induce osteoclastogenesis in co-cultures with monocytic cells. Successful purification of the CD14-negative stromal cells was confirmed via flow cytometric analysis and immunocytochemistry. Osteogenic media upregulated the expression of osteocalcin, suggesting an osteoblastic lineage of the GCTB stromal cells. The effects of the Wnt pathway agonist, SB415286, and recombinant human bone morphogenetic protein (BMP)-2 on osteoblastogenesis varied among samples. Notably, osteogenic media and SB415286 reversed the receptor activator of NF-κB ligand (RANKL)/osteoprotegerin (OPG) expression ratio resulting in diminished osteoclastogenic capacity. Recombinant human BMP2 had the opposite effect, resulting in enhanced and sustained support of osteoclastogenesis. Targeting the giant cell tumor stromal cell may be an effective adjunct to existing anti-resorptive strategies. 相似文献
133.
A study of Calypso, Cephalanthera, Corallorhiza and Epipactis seeds suggests that morphometric analysis can be useful as a taxonomic tool and an indicator of developmental patterns. 相似文献
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Devine MJ Kaganovich A Ryten M Mamais A Trabzuni D Manzoni C McGoldrick P Chan D Dillman A Zerle J Horan S Taanman JW Hardy J Marti-Masso JF Healy D Healey D Schapira AH Wolozin B Bandopadhyay R Cookson MR van der Brug MP Lewis PA 《PloS one》2011,6(7):e22489
Point mutations in LRRK2 cause autosomal dominant Parkinson's disease. Despite extensive efforts to determine the mechanism of cell death in patients with LRRK2 mutations, the aetiology of LRRK2 PD is not well understood. To examine possible alterations in gene expression linked to the presence of LRRK2 mutations, we carried out a case versus control analysis of global gene expression in three systems: fibroblasts isolated from LRRK2 mutation carriers and healthy, non-mutation carrying controls; brain tissue from G2019S mutation carriers and controls; and HEK293 inducible LRRK2 wild type and mutant cell lines. No significant alteration in gene expression was found in these systems following correction for multiple testing. These data suggest that any alterations in basal gene expression in fibroblasts or cell lines containing mutations in LRRK2 are likely to be quantitatively small. This work suggests that LRRK2 is unlikely to play a direct role in modulation of gene expression, although it remains possible that this protein can influence mRNA expression under pathogenic cicumstances. 相似文献
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Nair GM Healey JS Gordon E Divakaramenon S Morillo CA 《Indian pacing and electrophysiology journal》2011,11(4):120-125
Introduction
A patient with D-TGA and surgical repair (Mustard''s procedure) presented with appropriate ICD shocks due to monomorphic ventricular tachycardia, refractory to antiarrhythmic medications.Methods and Results
The patient underwent an electrophysiological study and catheter ablation for the VT. Substrate and pace mapping techniques, with the help of an electroanatomical mapping system, was used to localize and ablate the tachycardia successfully.Conclusions
In patients with D-TGA and Mustard''s repair, scar tissue resulting from VSD repair can act as a substrate for recurrent VT. Catheter ablation of VT is useful in management of VT that occurs despite antiarrhythmic therapy and/or when it is unstable. 相似文献140.
Catrin H. Middleton William Irving John F. R. Robertson Andrea Murray Celine B. Parsy-Kowalska Isabel K. Macdonald Jane McElveen Jared Allen Graham F. Healey Brian J. Thomson Stephen J. Ryder Stefan Holdenrieder Caroline J. Chapman 《PloS one》2014,9(8)