全文获取类型
收费全文 | 400篇 |
免费 | 56篇 |
出版年
2021年 | 6篇 |
2020年 | 5篇 |
2019年 | 5篇 |
2018年 | 5篇 |
2017年 | 5篇 |
2016年 | 7篇 |
2015年 | 16篇 |
2014年 | 14篇 |
2013年 | 15篇 |
2012年 | 29篇 |
2011年 | 27篇 |
2010年 | 19篇 |
2009年 | 16篇 |
2008年 | 12篇 |
2007年 | 28篇 |
2006年 | 25篇 |
2005年 | 15篇 |
2004年 | 17篇 |
2003年 | 12篇 |
2002年 | 8篇 |
2001年 | 12篇 |
2000年 | 15篇 |
1999年 | 10篇 |
1998年 | 12篇 |
1997年 | 8篇 |
1996年 | 3篇 |
1995年 | 5篇 |
1994年 | 4篇 |
1993年 | 7篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1989年 | 9篇 |
1988年 | 16篇 |
1987年 | 7篇 |
1986年 | 2篇 |
1985年 | 9篇 |
1984年 | 4篇 |
1983年 | 6篇 |
1982年 | 3篇 |
1980年 | 3篇 |
1979年 | 7篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1954年 | 1篇 |
1950年 | 1篇 |
1944年 | 1篇 |
1932年 | 1篇 |
1903年 | 1篇 |
1902年 | 1篇 |
排序方式: 共有456条查询结果,搜索用时 15 毫秒
71.
Jin G Zhou X Wang H Zhao H Cui K Zhang XS Chen L Hazen SL Li K Wong ST 《Journal of proteome research》2008,7(9):4013-4021
The mass spectrometry (MS) technology in clinical proteomics is very promising for discovery of new biomarkers for diseases management. To overcome the obstacles of data noises in MS analysis, we proposed a new approach of knowledge-integrated biomarker discovery using data from Major Adverse Cardiac Events (MACE) patients. We first built up a cardiovascular-related network based on protein information coming from protein annotations in Uniprot, protein-protein interaction (PPI), and signal transduction database. Distinct from the previous machine learning methods in MS data processing, we then used statistical methods to discover biomarkers in cardiovascular-related network. Through the tradeoff between known protein information and data noises in mass spectrometry data, we finally could firmly identify those high-confident biomarkers. Most importantly, aided by protein-protein interaction network, that is, cardiovascular-related network, we proposed a new type of biomarkers, that is, network biomarkers, composed of a set of proteins and the interactions among them. The candidate network biomarkers can classify the two groups of patients more accurately than current single ones without consideration of biological molecular interaction. 相似文献
72.
73.
74.
Borglin S Joyner D Jacobsen J Mukhopadhyay A Hazen TC 《Journal of microbiological methods》2009,76(2):159-168
Growing anaerobic microorganisms in phenotypic microarrays (PM) and 96-well microtiter plates is an emerging technology that allows high throughput survey of the growth and physiology and/or phenotype of cultivable microorganisms. For non-model bacteria, a method for phenotypic analysis is invaluable, not only to serve as a starting point for further evaluation, but also to provide a broad understanding of the physiology of an uncharacterized wild-type organism or the physiology/phenotype of a newly created mutant of that organism. Given recent advances in genetic characterization and targeted mutations to elucidate genetic networks and metabolic pathways, high-throughput methods for determining phenotypic differences are essential. Here we outline challenges presented in studying the physiology and phenotype of a sulfate-reducing anaerobic delta proteobacterium, Desulfovibrio vulgaris Hildenborough. Modifications of the commercially available OmniLog system (Hayward, CA) for experimental setup, and configuration, as well as considerations in PM data analysis are presented. Also highlighted here is data viewing software that enables users to view and compare multiple PM data sets. The PM method promises to be a valuable strategy in our systems biology approach to D. vulgaris studies and is readily applicable to other anaerobic and aerobic bacteria. 相似文献
75.
76.
Zhiping Wu Valentin Gogonea Xavier Lee Matthew A. Wagner Xin-Min Li Ying Huang Arundhati Undurti Roland P. May Michael Haertlein Martine Moulin Irina Gutsche Giuseppe Zaccai Joseph A. DiDonato Stanley L. Hazen 《The Journal of biological chemistry》2009,284(52):36605-36619
High density lipoprotein (HDL), the carrier of so-called “good” cholesterol, serves as the major athero-protective lipoprotein and has emerged as a key therapeutic target for cardiovascular disease. We applied small angle neutron scattering (SANS) with contrast variation and selective isotopic deuteration to the study of nascent HDL to obtain the low resolution structure in solution of the overall time-averaged conformation of apolipoprotein AI (apoA-I) versus the lipid (acyl chain) core of the particle. Remarkably, apoA-I is observed to possess an open helical shape that wraps around a central ellipsoidal lipid phase. Using the low resolution SANS shapes of the protein and lipid core as scaffolding, an all-atom computational model for the protein and lipid components of nascent HDL was developed by integrating complementary structural data from hydrogen/deuterium exchange mass spectrometry and previously published constraints from multiple biophysical techniques. Both SANS data and the new computational model, the double superhelix model, suggest an unexpected structural arrangement of protein and lipids of nascent HDL, an anti-parallel double superhelix wrapped around an ellipsoidal lipid phase. The protein and lipid organization in nascent HDL envisages a potential generalized mechanism for lipoprotein biogenesis and remodeling, biological processes critical to sterol and lipid transport, organismal energy metabolism, and innate immunity. 相似文献
77.
Christopher B. Walker Sergey Stolyar Dylan Chivian Nicolas Pinel Jeffrey A. Gabster Paramvir S. Dehal Zhili He Zamin Koo Yang Huei-Che B. Yen Jizhong Zhou Judy D. Wall Terry C. Hazen Adam P. Arkin David A. Stahl 《Environmental microbiology》2009,11(9):2244-2252
The genome of Desulfovibrio vulgaris strain DePue, a sulfate-reducing Deltaproteobacterium isolated from heavy metal-impacted lake sediment, was completely sequenced and compared with the type strain D. vulgaris Hildenborough. The two genomes share a high degree of relatedness and synteny, but harbour distinct prophage and signatures of past phage encounters. In addition to a highly variable phage contribution, the genome of strain DePue contains a cluster of open-reading frames not found in strain Hildenborough coding for the production and export of a capsule exopolysaccharide, possibly of relevance to heavy metal resistance. Comparative whole-genome microarray analysis on four additional D. vulgaris strains established greater interstrain variation within regions associated with phage insertion and exopolysaccharide biosynthesis. 相似文献
78.
79.
Environmental contamination with a variety of pollutants has prompted the development of effective bioremediation strategies. But how can these processes be best monitored and controlled? One avenue under investigation is the development of stress response systems as tools for effective and general process control. Although the microbial stress response has been the subject of intensive laboratory investigation, the environmental reflection of the laboratory response to specific stresses has been little explored. However, it is only within an environmental context, in which microorganisms are constantly exposed to multiple changing environmental stresses, that there will be full understanding of microbial adaptive resiliency. Knowledge of the stress response in the environment will facilitate the control of bioremediation and other processes mediated by complex microbial communities. 相似文献
80.
Cindy H. Wu Bram Sercu Laurie C. Van De Werfhorst Jakk Wong Todd Z. DeSantis Eoin L. Brodie Terry C. Hazen Patricia A. Holden Gary L. Andersen 《PloS one》2010,5(6)