全文获取类型
收费全文 | 381篇 |
免费 | 41篇 |
出版年
2023年 | 4篇 |
2022年 | 8篇 |
2021年 | 15篇 |
2020年 | 18篇 |
2019年 | 12篇 |
2018年 | 19篇 |
2017年 | 11篇 |
2016年 | 16篇 |
2015年 | 31篇 |
2014年 | 39篇 |
2013年 | 33篇 |
2012年 | 37篇 |
2011年 | 33篇 |
2010年 | 14篇 |
2009年 | 15篇 |
2008年 | 29篇 |
2007年 | 16篇 |
2006年 | 22篇 |
2005年 | 7篇 |
2004年 | 9篇 |
2003年 | 2篇 |
2002年 | 10篇 |
2001年 | 2篇 |
1999年 | 3篇 |
1998年 | 3篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1985年 | 1篇 |
1972年 | 1篇 |
1971年 | 2篇 |
排序方式: 共有422条查询结果,搜索用时 796 毫秒
51.
Lam H Patel S Wong J Chu J Lau A Li A Li S 《Biochemical and biophysical research communications》2008,372(4):601-606
Human embryonic stem cells (hESC) are pluripotent, and can be directed to differentiate into different cell types for therapeutic applications. To expand hESCs, it is desirable to maintain hESC growth without differentiation. As hESC colonies grow, differentiated cells are often found at the periphery of the colonies, but the underlying mechanism is not well understood. Here, we utilized micropatterning techniques to pattern circular islands or strips of matrix proteins, and examined the spatial pattern of hESC renewal and differentiation. We found that micropatterned matrix restricted hESC differentiation at colony periphery but allowed hESC growth into multiple layers in the central region, which decreased hESC proliferation and induced hESC differentiation. In undifferentiated hESCs, β-catenin primarily localized at cell-cell junctions but not in the nucleus. The amount of β-catenin in differentiating hESCs at the periphery of colonies or in multiple layers decreased significantly at cell-cell junctions. Consistently, knocking down β-catenin decreased Oct-4 expression in hESCs. These results indicate that localized decrease of β-catenin contributes to the spatial pattern of differentiation in hESC colonies. 相似文献
52.
53.
54.
Michael Burton John D. Perry Stephen P. Stanforth Hayley J. Turner 《Bioorganic & medicinal chemistry》2018,26(17):4841-4849
The preparation and evaluation of chromogenic substrates for detecting bacterial glycosidase enzymes is reported. These substrates are monoglycoside derivatives of the metal chelators catechol, 2,3-dihydroxynaphthalene (DHN) and 6,7-dibromo-2,3-dihydroxynaphthalene (6,7-dibromo-DHN). When hydrolysed by appropriate bacterial enzymes these substrates produced coloured chelates in the presence of ammonium iron(III) citrate, thus enabling bacterial detection. A β-d-riboside of DHN and a β-d-glucuronide derivative of 6,7-dibromo-DHN were particularly effective for the detection of S. aureus and E. coli respectively. 相似文献
55.
56.
57.
Nicole J. Lake Bryn D. Webb David A. Stroud Tara R. Richman Benedetta Ruzzenente Alison G. Compton Hayley S. Mountford Juliette Pulman Coralie Zangarelli Marlene Rio Nathalie Boddaert Zahra Assouline Mingma D. Sherpa Eric E. Schadt Sander M. Houten James Byrnes Elizabeth M. McCormick Zarazuela Zolkipli-Cunningham David R. Thorburn 《American journal of human genetics》2018,102(4):713
58.
59.
60.
Hayley A. Lawrence Craig D. Millar Graeme A. Taylor Linda D. Macdonald David M. Lambert 《Journal of avian biology》2008,39(3):359-363
The Chatham Island taiko Pterodroma magentae (tchaik) is one of the World's most endangered seabirds with a population size of between 120–150 individuals that includes only 8–15 breeding pairs. Molecular techniques were used to identify the sex of taiko, which is difficult to assign morphologically. Blood samples were obtained from almost the entire known living population and from some birds now thought to be dead. We report an approximately even sex ratio in taiko chicks and adults associated with breeding burrows, but a large male-biased ratio in non-breeding adult birds caught on the ground. This finding suggests that unpaired males may be having difficulty in attracting females to burrows and that this situation may be an example of the Allee effect, that reduced density of potential mates acts to decrease population productivity. Identification of the sex of taiko using a molecular technique has important implications for the conservation management of this critically endangered species, including the future transfer of taiko chicks to a predator-excluded breeding site. 相似文献