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Luc Verschaeve Hayet Edziri Roel Anthonissen Dalenda Boujnah Fethia Skhiri Mahjoub Aouni Maha Mastouri 《Acta Physiologiae Plantarum》2017,39(9):206
Ruscus hypophyllum L. is a rare Mediterranean plant which is used in the traditional medicine. We studied its phenolic content and in vitro toxicity and genotoxicity using the neutral red uptake (NRU) test, the bacterial Vitotox test, and the comet assay in human C3A hepatic cells. Aqueous leaf and fruit extracts were investigated. Antigenotoxicity against 4-nitroquinoline-oxide (4NQO, 0.4 µg/mL) and Benzo(α)pyrene (BaP, 800 µg/mL) was also investigated with the Vitotox test. The extracts appeared to be genotoxic only at high exposure levels in the comet assay. There was no indication of a genotoxic activity in the Vitotox test and also no indication of antigenotoxicity. The moderate polyphenol content may provide an explanation for the absence of antigenotoxicity. 相似文献
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Fournel S Neichel S Dali H Farci S Maillère B Briand JP Muller S 《Journal of immunology (Baltimore, Md. : 1950)》2003,171(2):636-644
We have previously reported that peptide 88-99 of histone H4 represents a minimal T cell epitope recognized by Th cells from nonautoimmune BALB/c (H-2(d/d)) mice immunized with nucleosomes. In this study, we tested a panel of overlapping peptides spanning the whole sequences of H4 and H3 for recognition by CD4(+) T cells from unprimed (New Zealand Black (NZB) x New Zealand White (NZW))F(1) lupus mice (H-2(d/z)). None of the 11 H4 peptides was recognized by CD4(+) T cells from (NZB x NZW)F(1) mice. In contrast, these cells proliferated and secreted IL-2, IL-10, and IFN-gamma upon ex vivo stimulation with H3 peptides representing sequences 53-70, 64-78, and 68-85. Peptides 56-73 and 61-78 induced the production of IFN-gamma and IL-10, respectively, without detectable proliferation, suggesting that they may act as partial agonist of the TCR. Th cells from unprimed BALB/c mice and other lupus-prone mice such as SNF(1) (H-2(d/q)) and MRL/lpr (H-2(k/k)) mice did not recognize any peptides present within the H3 region 53-85. We further demonstrated that immunization of normal BALB/c mice with syngeneic liver nucleosomes and spleen apoptotic cells, but not with nonapoptotic syngeneic cells, induced Th cell responses against several peptides of the H3 region 53-85. Moreover, we found that this conserved region of H3, which is accessible at the surface of nucleosomes, is targeted by Abs from (NZB x NZW)F(1) mice and lupus patients, and contains motifs recognized by several distinct HLA-DR molecules. It might thus be important in the self-tolerance breakdown in lupus. 相似文献
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Mariem Ben Said M’hamed Grati Takahiro Ishimoto Bing Zou Imen Chakchouk Qi Ma Qi Yao Bouthaina Hammami Denise Yan Rahul Mittal Noritaka Nakamichi Abdelmonem Ghorbel Lingling Neng Mustafa Tekin Xiao Rui Shi Yukio Kato Saber Masmoudi Zhongmin Lu Mounira Hmani Xuezhong Liu 《Human genetics》2016,135(5):513-524
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Mariam Siala Radhouane Gdoura Mohamed Younes Hela Fourati Ilhem Cheour Nihel Meddeb Naceur Bargaoui Sofien Baklouti Slaheddine Sellami Markus Rihl & Adnene Hammami 《FEMS immunology and medical microbiology》2009,55(2):178-186
We aimed to determine the frequency of Chlamydia trachomatis DNA in the synovial compartment of 34 arthritic patients. Chlamydia trachomatis DNA was detected using a nested PCR targeting the cryptic plasmid, the 16S rRNA gene and the outer membrane protein 1 gene. The presence of serum immunoglobulin (Ig)G and IgA antibodies against C. trachomatis was studied by a microimmunofluorescence assay and by an enzyme-linked immunosorbent assay, respectively. Synovial samples from 20 of 34 (59%) patients [nine with reactive arthritis (ReA), seven with undifferentiated oligoarthritis (UOA), two with rheumatoid arthritis and two with osteoarthritis] were positive for at least one C. trachomatis DNA sequence by nested PCR. The high sensitivity results most likely from the combination of a standardized automated MagNA Pure extraction method, PCR targeting three different C. trachomatis genes and the screening for C. trachomatis in synovial tissue and fluid samples. There was no correlation between the presence of C. trachomatis DNA in the joint and a Chlamydia -specific serologic response. Our data support that PCR is the method of choice to establish the diagnosis of Chlamydia -induced arthritis in patients with ReA. We suggest that this diagnosis might also be considered in C. trachomatis -positive patients previously classified as UOA. 相似文献
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Newborn rat adrenocortical cells were successfully cultured in a serum free carrier protein free medium (SPFM) by using -cyclodextrin as a cholesterol carrier and have expressed corticosteroid biosynthesis in this medium. A stable inclusion complex of cholesterol--cyclodextrin with a molar ratio of almost 1 was obtained for a 5 × 10–5 mol/1 -cyclodextrin concentration. Cell cultures incubated with [4-14C] cholesterol--cyclodextrin in SPFM produced, under ACTH stimulation, various 14C labeled steroids with a predominance of corticosterone and 18-hydroxy-11-deoxycorticosterone. As measured by gas chromatography and mass spectrometry, the ratio between corticosteroids (21-hydroxylated steroids) and 20-reduced steroids produced in SPFM with cholesterol--cyclodextrin was equal to 1.8. This corresponds to a value of 3.6 times higher than that found in the serum free medium with cholesterol-albumin. Consequently, the chemically defined SPFM with cholesterol--cyclodextrin used in this study is more suitable for corticosteroidogenesis by adrenal cells in culture than a serum free medium with cholesterol-albumin.Abbreviations -CD
-cyclodextrin
- ACTH
adrenocorticotropic hormone
- 20-dihydroprogesterone
20-hydroxy-4-pregnene-3-one
- 11-hydroxy-20-dihydroprogesterone
11, 20-dihydroxy-4-pregnene-3-one
- 11-hydroxyprogesterone
11-hydroxy-4-pregnene-3,20-dione
- C--CD
cholesterol--cyclodextrin complex
- corticosterone
11,21-dihydroxy-4-pregnene-3,20-dione
- deoxycorticosterone
21-hydroxy-4-pregnene-3,20-dione
- 18-hydroxy-11-deoxycorticosterone
18,21-dihydroxy-4-pregnene-3,20-dione
- 18-hydroxy-20-dihydroprogesterone
18-20-dihydroxy-4-pregnene-3-one
- 18-hydroxyprogesterone
18-hydroxy4-pregnene-3,20-dione
- progesterone
4-pregnene-3,20-dione
- SFM-S
serum-free medium
- SPFM
serum-free protein-free medium
- SSM
serum supplemented medium 相似文献
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Edziri Hayet Mastouri Maha Ammar Samia Matieu Mata Patrich Gros Hiar Raida Mahjoub Mohamed Ali Ali Si Mohamed Laurent Gutmann Zine Mighri Aouni Mahjoub 《World journal of microbiology & biotechnology》2008,24(12):2933-2940
Antimicrobial, antioxidant, and antiviral activities of flower extracts of Retama raetam Forssk. Webb (Fabaceae) were screened both from standard and isolated Gram-positive and Gram-negative bacteria by solid medium
technique. Oxacillin, Amoxicillin, Ticarcillin, Cefotaxim, and Amphotericin were used as the control agents. The antiviral
activity was evaluated against human cytomegalovirus (HCMV) strain AD-169 (ATCC Ref. VR 538) and coxsackie B virus type 3
(CoxB-3) using a cytopathic effect (CPE) reduction assay. The antioxidant activity was evaluated using two tests: 1,1-diphenyl-2-picrylhydrazyl
(DPPH) free-radical scavenging and the ammonium thiocyanate methods. All extracts were characterized quantitatively for the
presence of polyphenols, flavonoids, and tannins. Of the extracts tested, butanol and ethyl acetate extracts showed important
antibacterial activity against Gram-positive and Gram-negative bacteria but only moderate antifungal activity. Methanol extract
exhibited moderate antiviral activity against HCMV with IC50 of 250 μg/ml. Ethyl acetate, chloroform, and methanol fractions were found to cause significant free-radical-scavenging effects
in both assays. These results may suggest that R. raetam flowers could be used as a natural preservative ingredient in the food and/or pharmaceutical industries. 相似文献