全文获取类型
收费全文 | 80篇 |
免费 | 3篇 |
出版年
2021年 | 2篇 |
2020年 | 1篇 |
2018年 | 2篇 |
2017年 | 3篇 |
2015年 | 1篇 |
2014年 | 3篇 |
2013年 | 2篇 |
2012年 | 8篇 |
2011年 | 8篇 |
2010年 | 6篇 |
2009年 | 6篇 |
2008年 | 3篇 |
2007年 | 5篇 |
2006年 | 5篇 |
2005年 | 2篇 |
2004年 | 1篇 |
2003年 | 2篇 |
2002年 | 3篇 |
1997年 | 1篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1980年 | 1篇 |
1978年 | 3篇 |
1976年 | 2篇 |
1973年 | 2篇 |
排序方式: 共有83条查询结果,搜索用时 31 毫秒
41.
Haydn Kuchel Rebecca Fox Jason Reinheimer Lee Mosionek Nicholas Willey Harbans Bariana Stephen Jefferies 《Molecular breeding : new strategies in plant improvement》2007,20(4):295-308
A number of useful marker-trait associations have been reported for wheat. However the number of publications detailing the
integrated and pragmatic use of molecular markers in wheat breeding is limited. A previous report by some of these authors
showed how marker-assisted selection could increase the genetic gain and economic efficiency of a specific breeding strategy.
Here, we present a practical validation of that study. The target of this breeding strategy was to produce wheat lines derived
from an elite Australian cultivar ‘Stylet’, with superior dough properties and durable rust resistance donated from ‘Annuello’.
Molecular markers were used to screen a BC1F1 population produced from a cross between the recurrent parent ‘Stylet’ and the donor parent ‘Annuello’ for the presence of
rust resistance genes Lr34/Yr18 and Lr46/Yr29. Following this, marker-assisted selection was applied to haploid plants, prior to chromosome doubling with cochicine, for
the rust resistance genes Lr24/Sr24, Lr34/Yr18, height reducing genes, and for the grain protein genes Glu-D1 and Glu-A3. In general, results from this study agreed with those of the simulation study. Genetic improvement for rust resistance was
greatest when marker selection was applied on BC1F1 individuals. Introgression of both the Lr34/Yr18 and Lr46/Yr29 loci into the susceptible recurrent parent background resulted in substantial improvement in leaf rust and stripe rust resistance
levels. Selection for favourable glutenin alleles significantly improved dough resistance and dough extensibility. Marker-assisted
selection for improved grain yield, through the selection of recurrent parent genome using anonymous markers, only marginally
improved grain yield at one of the five sites used for grain yield assessment. In summary, the integration of marker-assisted
selection for specific target genes, particularly at the early stages of a breeding programme, is likely to substantially
increase genetic improvement in wheat. 相似文献
42.
Charu Aggarwal Keshav Saini Elluri Seetharami Reddy Mohit Singla Kaustuv Nayak Yadya M. Chawla Deepti Maheshwari Prabhat Singh Pragati Sharma Priya Bhatnagar Sanjeev Kumar Kamalvishnu Gottimukkala Harekrushna Panda Sivaram Gunisetty Carl W. Davis Haydn Thomas Kissick Sushil Kumar Kabra Rakesh Lodha Guruprasad R. Medigeshi Rafi Ahmed Kaja Murali-Krishna Anmol Chandele 《Journal of virology》2021,95(23)
43.
Haydn Didier 《BMJ (Clinical research ed.)》1986,292(6537):1741
44.
Agnieszka K. Rzadzinska Elisa M. Nevalainen Haydn M. Prosser Pekka Lappalainen Karen P. Steel 《PloS one》2009,4(9)
In vertebrates hearing is dependent upon the microvilli-like mechanosensory stereocilia and their length gradation. The staircase-like organization of the stereocilia bundle is dynamically maintained by variable actin turnover rates. Two unconventional myosins were previously implicated in stereocilia length regulation but the mechanisms of their action remain unknown. MyosinXVa is expressed in stereocilia tips at levels proportional to stereocilia length and its absence produces staircase-like bundles of very short stereocilia. MyosinVIIa localizes to the tips of the shorter stereocilia within bundles, and when absent, the stereocilia are abnormally long. We show here that myosinVIIa interacts with twinfilin-2, an actin binding protein, which inhibits actin polymerization at the barbed end of the filament, and that twinfilin localization in stereocilia overlaps with myosinVIIa. Exogenous expression of myosinVIIa in fibroblasts results in a reduced number of filopodia and promotes accumulation of twinfilin-2 at the filopodia tips. We hypothesize that the newly described interaction between myosinVIIa and twinfilin-2 is responsible for the establishment and maintenance of slower rates of actin turnover in shorter stereocilia, and that interplay between complexes of myosinVIIa/twinfilin-2 and myosinXVa/whirlin is responsible for stereocilia length gradation within the bundle staircase. 相似文献
45.
46.
47.
Vernet N Mahadevaiah SK Ojarikre OA Longepied G Prosser HM Bradley A Mitchell MJ Burgoyne PS 《Current biology : CB》2011,21(9):787-793
During male but not female mammalian meiosis, there is efficient apoptotic elimination of cells with unpaired (univalent) chromosomes at the first meiotic metaphase (MI) [1]. Apoptotic elimination of MI spermatocytes is seen in response to the univalent X chromosome of XSxr(a)O male mice [2], in which the X chromosome carries Sxr(a) [3, 4], the Y-chromosome-derived sex-reversal factor that includes the testis determinant Sry. Sxr(b) is an Sxr(a)-derived variant in which a deletion has removed six Y short-arm genes and created a Zfy2/Zfy1 fusion gene spanning the deletion breakpoint [4, 5]. XSxr(b)O males have spermatogonial arrest that can be overcome by the re-addition of Eif2s3y from the deletion as a transgene; however, XSxr(b)OEif2s3y transgenic males do not show the expected elimination of MI spermatocytes in response to the univalent [6]. Here we show that these XSxr(b)OEif2s3y males have an impaired apoptotic response with completion of the first meiotic division, but there is no second meiotic division. We then show that Zfy2 (but not the closely related Zfy1) is sufficient to reinstate the apoptotic response to the X univalent. These findings provide further insight into the basis for the much lower transmission of chromosomal errors originating at the first meiotic division in men than in women [7]. 相似文献
48.
Here, we sought to determine whether peptide vaccines designed harbor both class I as well as class II restricted antigenic motifs could concurrently induce CD4 and CD8 T cell activation against autologous tumor antigens. Based on our prior genome-wide interrogation of human prostate cancer tissues to identify genes over-expressed in cancer and absent in the periphery, we targeted SIM2 as a prototype autologous tumor antigen for these studies. Using humanized transgenic mice we found that the 9aa HLA-A*0201 epitope, SIM2237–245, was effective at inducing an antigen specific response against SIM2-expressing prostate cancer cell line, PC3. Immunization with a multi-epitope peptide harboring both MHC-I and MHC-II restricted epitopes induced an IFN-γ response in CD8 T cells to the HLA-A*0201-restricted SIM2237–245 epitope, and an IL-2 response by CD4 T cells to the SIM2240–254 epitope. This peptide was also effective at inducing CD8+ T-cells that responded specifically to SIM2-expressing tumor cells. Collectively, the data presented in this study suggest that a single peptide containing multiple SIM2 epitopes can be used to induce both a CD4 and CD8 T cell response, providing a peptide-based vaccine formulation for potential use in immunotherapy of various cancers. 相似文献
49.
Chen Y Sprung R Tang Y Ball H Sangras B Kim SC Falck JR Peng J Gu W Zhao Y 《Molecular & cellular proteomics : MCP》2007,6(5):812-819
The positively charged lysine residue plays an important role in protein folding and functions. Neutralization of the charge often has a profound impact on the substrate proteins. Accordingly all the known post-translational modifications at lysine have pivotal roles in cell physiology and pathology. Here we report the discovery of two novel, in vivo lysine modifications in histones, lysine propionylation and butyrylation. We confirmed, by in vitro labeling and peptide mapping by mass spectrometry, that two previously known acetyltransferases, p300 and CREB-binding protein, could catalyze lysine propionylation and lysine butyrylation in histones. Finally p300 and CREB-binding protein could carry out autopropionylation and autobutyrylation in vitro. Taken together, our results conclusively establish that lysine propionylation and lysine butyrylation are novel post-translational modifications. Given the unique roles of propionyl-CoA and butyryl-CoA in energy metabolism and the significant structural changes induced by the modifications, the two modifications are likely to have important but distinct functions in the regulation of biological processes. 相似文献
50.
Keith Biggadike Matilde Caivano Margaret Clackers Diane M. Coe George W. Hardy Davina Humphreys Haydn T. Jones David House Annette Miles-Williams Philip A. Skone Iain Uings Vicki Weller Iain M. McLay Simon J.F. Macdonald 《Bioorganic & medicinal chemistry letters》2009,19(16):4846-4850
Starting from a non-steroidal glucocorticoid agonist aryl pyrazole derivative, the NFκB agonist activity was optimised in an iterative process from pIC50 7.5 (for 7), to pIC50 10.1 (for 38E1). An explanation for the SAR observed based is presented along with a proposed docking of 38E1 into the active site of the glucocorticoid receptor. 相似文献