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181.
S. Nadeem Noreen Sher Akbar T. Hayat Awatif A. Hendi 《Computer methods in biomechanics and biomedical engineering》2013,16(11):987-993
In the present article, we have presented the peristaltic flow of an Oldroyd 8-constant fluid in an endoscope. The governing equations for the flow problem are simplified using long wavelength and low Reynold's number approximations. The solutions of the simplified problem are calculated using (i) Homotopy analysis method and (ii) Shooting method. The comparison of both the solutions shows a very good agreement between the results. The graphical results for the velocity field and stresses are presented to show the physical behaviour of all the parameters appearing in the problem. 相似文献
182.
Khan KM Rasheed M Zia-Ullah Hayat S Kaukab F Choudhary MI Atta-ur-Rahman Perveen S 《Bioorganic & medicinal chemistry》2003,11(7):1381-1387
Twenty-one hydrazides were synthesized by treating different esters with hydrazine hydrate. Substituted hydrazides were obtained by treating hydrazides with alkyl/aryl/acyl halides. Some of these compounds exhibit potential in vitro leishmanicidal activity. The structures of all the synthesized compounds were confirmed by spectroscopic analysis. 相似文献
183.
This paper investigates the steady hydromagnetic three-dimensional boundary layer flow of Maxwell fluid over a bidirectional stretching surface. Both cases of prescribed surface temperature (PST) and prescribed surface heat flux (PHF) are considered. Computations are made for the velocities and temperatures. Results are plotted and analyzed for PST and PHF cases. Convergence analysis is presented for the velocities and temperatures. Comparison of PST and PHF cases is given and examined. 相似文献
184.
The serine protease granzyme B (GrB) plays an important role in the immune defense mediated by cytotoxic lymphocytes. Recombinant derivatives of this pro-apoptotic protein fused to tumor-targeting ligands hold promise for cancer therapy, but their applicability may be limited by promiscuous binding to nontarget tissues via electrostatic interactions. Here, we investigated cell binding and specific cytotoxicity of chimeric molecules consisting of wild-type or surface-charge-modified human GrB and the natural EGFR ligand TGFα for tumor targeting. We mutated two cationic heparin-binding motifs responsible for electrostatic interactions of GrB with cell surface structures, and genetically fused the resulting GrBcs derivative to TGFα for expression in the yeast Pichia pastoris . Purified GrBcs-TGFα (GrBcs-T) and a corresponding fusion protein employing wild-type GrB (GrB-T) displayed similar enzymatic activity and targeted cytotoxicity against EGFR-overexpressing breast carcinoma cells in the presence of an endosomolytic reagent. However, unspecific binding of the modified GrBcs-T variant to EGFR-negative cells was dramatically reduced, preventing the sequestration by nontarget cells in mixed cell cultures and increasing tumor-cell specificity. Likewise, modification of the GrB domain alleviated unspecific extracellular effects such as cell detachment indicative of extracellular matrix degradation. Our data demonstrate improved selectivity and functionality of surface-charge-modified GrBcs, suggesting this strategy as a general approach for the development of optimized GrB fusion proteins for therapeutic applications. 相似文献
185.
The purpose of this study was to determine whether biocompatible and biodegradable vasoactive intestinal peptide-grafted sterically stabilized phospholipid mixed nanomicelles (VIP-SSMM; size, approximately 15 nm), a novel nanosized actively targeted drug delivery platform for breast cancer, accumulate in human MCF-7 breast cancer cells. Using hydrophobic CdSe/ZnS quantum dots (QD), we found that QD-loaded VIP-SSMM accumulated significantly faster and in greater quantity in MCF-7 cells than did QD-loaded SSMM alone (p<0.05). This process was mediated, in part, by VIP receptors because excess human VIP, but not PACAP(6-38) or galanin, significantly attenuated this response (p<0.05). Taken together, these data indicate that VIP-SSMM are actively targeted to human breast cancer cells through VIP receptors. We suggest that VIP-SSMM could be used as an actively targeted nanosized drug delivery platform for breast cancer cells over-expressing VIP receptors. 相似文献
186.
Helospectin I and II, two closely related mammalian neuropeptides of the secretin/glucagons/vasoactive intestinal peptide (VIP) superfamily of peptides, are co-localized with VIP in nerve fibers surrounding vascular smooth muscle. However, the role if any, VIP receptors play in transducing the vasorelaxant effects of helospectin I and II in the intact peripheral microcirculation is uncertain. The purpose of this study was to determine whether helospectin I and II elicit vasodilation in the intact peripheral microcirculation and, if so, whether this response is mediated, in part, by VIP or pituitary adenylate cyclase activating peptide (PACAP) receptor engagement, and through local elaboration of cyclooxygenase products of arachidonic acid metabolism. Using intravital microscopy, we found that suffusion of helospectin I and II (each, 1.0 nmol) evoked potent vasodilation and of similar magnitude in the intact hamster cheek pouch microcirculation (P < 0.05). Suffusion of 0.1 nmol helospectin I and II had no significant effects on arteriolar diameter. Pretreatment with VIP(10-28), a VPAC1/VPAC2 receptor antagonist, or PACAP(6-38), a PAC1/VPAC2 receptor antagonist, had no significant effects on helospectin I- and II-induced responses. In addition, pretreatment with indomethacin had no significant effects on helospectin I- and II-induced vasodilation. Collectively, these data indicate that helospectin I and II evoke potent vasodilation in the intact peripheral microcirculation that is not transduced by VIP or PACAP receptors nor through cyclooxygenase products of arachidonic acid metabolism. 相似文献
187.
Rifat Hayat Iftikhar Ahmed Jayoung Paek Yeseul Sin Muhammad Ehsan Muhammad Iqbal Akira Yokota Young H. Chang 《Annals of microbiology》2014,64(3):1081-1088
A Gram-negative, motile, rod-shaped, endospore-forming bacterial strain, designated as NCCP-36T, was isolated from the compost of fruit and vegetable wastes. The strain NCCP-36T grew within a temperature range of 10–45?○C (optimum 28?○C) and a pH range of 6.5–8.5 (optimum 7.0), and its cells tolerated <50 mM boron (optimum growth without boron) and 0–5 % NaCl (w/v) in tryptic soya broth medium. Based on comparative analysis of 16S rRNA gene sequence, strain NCCP-36T showed the highest similarity to Lysinibacillus sinduriensis BLB-1T (97.52 %) and L. xylanilyticus XDB9T (96.96 %), and <97 % similarity with other closely related taxa. However, DNA–DNA relatedness between strain NCCP-36T and the closely related type strains of genus Lysinibacillus was ≤37 %. Phylogenetic and chemotaxonomic analyses [major polar lipids: diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phospholipids; predominant menaquinone: MK-7; major cellular fatty acids: iso-C15:0, antieso-C15:0, and iso-C16:0; DNA G+C contents: 37 mol %; Lys-Asp (type A4α) in cell-wall peptidoglycans as diagnostic amino acids] also support the affiliation of strain NCCP-36T to genus Lysinibacillus. Based upon DNA–DNA relatedness as well as distinctive chemotaxonomic, phylogenetic, and genotypic data, we conclude that strain NCCP-36T belongs to a novel species of genus Lysinibacillus, for which the name Lysinibacillus composti sp. nov. is proposed. The type strain is NCCP-36T (JCM 18777T?=?KCTC 13796T?=?DSMZ 24785T). 相似文献
188.
The plants of mung bean (Vigna radiata L. Wilczek) were raised from the seeds soaked in water (control), IAA or 4-C-IAA (10−6, 10−8 or 10−10 M) for 8 or 12 h. The plants were allowed to grow in a net house and were sampled at 30 and 45 days after sowing (DAS). Both
IAA and 4-Cl-IAA significantly affected the growth (length, fresh and dry mass of roots and shoots), the number of nodules,
their fresh and dry mass and the activity of nitrogenase. However, the contents of nitrogen and carbohydrate exhibited a decrease
in response to both the auxins. 4-Cl-IAA, at a concentration of 10−8 M, generated the best response. Moreover, 4-Cl-IAA at other two concentrations (10−6 and 10−10 M) was much more active than any of the IAA concentration used. 相似文献
189.
The aim here is to investigate the effects of convective heat and mass transfer in the flow of Eyring-Powell fluid past an inclined exponential stretching surface. Mathematical formulation and analysis have been performed in the presence of Soret, Dufour and thermal radiation effects. The governing partial differential equations corresponding to the momentum, energy and concentration are reduced to a set of non-linear ordinary differential equations. Resulting nonlinear system is computed for the series solutions. Interval of convergence is determined. Physical interpretation is seen for the embedded parameters of interest. Skin friction coefficient, local Nusselt number and local Sherwood number are numerically computed and examined. 相似文献
190.
The purpose of this study was to determine whether vasoactive intestinal peptide (VIP), a pleiotropic amphipathic peptide, interacts with rigid liposomes composed of gel phase phospholipids. We found that incubation of VIP with small unilamellar gel phase liposomes composed of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) and egg phosphatidylglycerol (ePG) for 2h at room temperature had no significant effects on VIP secondary structure. Moreover, suffusion of VIP (0.01, 0.1 and 1.0nmol) incubated in saline or with DPPC/ePG liposomes (size, 30 and 100nm) for 2h at room temperature or 4 degrees C onto the intact hamster cheek pouch microcirculation elicited a similar concentration-dependent vasodilation except for 0.01nmol VIP (P<0.05). By contrast, incubation of VIP with gel phase liposomes overnight at 4 degrees C significantly potentiated vasodilation evoked by all three concentrations of the peptide in comparison to aqueous VIP (P<0.05). VIP-induced vasodilation was liposome size-independent. The ratio of VIP to phospholipids in DPPC/ePG liposomes was concentration-independent. Collectively, these data indicate that short-term interactions of VIP with rigid phospholipid bilayers are limited resulting in only modest effects on VIP vasoreactivity in vivo. 相似文献