全文获取类型
收费全文 | 12275篇 |
免费 | 723篇 |
国内免费 | 5篇 |
出版年
2022年 | 65篇 |
2021年 | 116篇 |
2020年 | 81篇 |
2019年 | 104篇 |
2018年 | 138篇 |
2017年 | 124篇 |
2016年 | 235篇 |
2015年 | 320篇 |
2014年 | 352篇 |
2013年 | 790篇 |
2012年 | 695篇 |
2011年 | 657篇 |
2010年 | 423篇 |
2009年 | 389篇 |
2008年 | 681篇 |
2007年 | 722篇 |
2006年 | 683篇 |
2005年 | 627篇 |
2004年 | 734篇 |
2003年 | 618篇 |
2002年 | 646篇 |
2001年 | 295篇 |
2000年 | 271篇 |
1999年 | 245篇 |
1998年 | 163篇 |
1997年 | 130篇 |
1996年 | 113篇 |
1995年 | 108篇 |
1994年 | 112篇 |
1993年 | 104篇 |
1992年 | 178篇 |
1991年 | 161篇 |
1990年 | 174篇 |
1989年 | 175篇 |
1988年 | 154篇 |
1987年 | 130篇 |
1986年 | 120篇 |
1985年 | 102篇 |
1984年 | 102篇 |
1983年 | 79篇 |
1982年 | 70篇 |
1981年 | 68篇 |
1980年 | 66篇 |
1979年 | 84篇 |
1978年 | 63篇 |
1977年 | 66篇 |
1975年 | 50篇 |
1974年 | 50篇 |
1973年 | 48篇 |
1972年 | 51篇 |
排序方式: 共有10000条查询结果,搜索用时 164 毫秒
11.
A new snake-eel,Apterichtus keramanus, is described on the basis of a single 276-mm TL specimen trawled from the coast of Kerama Islands, Okinawa Prefecture, Japan.
The species is unique in the genus in having the posterior nostril opening entirely inside the mouth and a dark band running
from the anteroventral margin of the eye to the upper lip. 相似文献
12.
13.
14.
Kei Watanabe Kenta Wada Tomoko Ohashi Saki Okubo Kensuke Takekuma Ryoichi Hashizume Jun-Ichi Hayashi Tadao Serikawa Takashi Kuramoto Yoshiaki Kikkawa 《PloS one》2012,7(11)
We discovered a new cataract mutation, kfrs4, in the Kyoto Fancy Rat Stock (KFRS) background. Within 1 month of birth, all kfrs4/kfrs4 homozygotes developed cataracts, with severe opacity in the nuclei of the lens. In contrast, no opacity was observed in the kfrs4/+ heterozygotes. We continued to observe these rats until they reached 1 year of age and found that cataractogenesis did not occur in kfrs4/+ rats. To define the histological defects in the lenses of kfrs4 rats, sections of the eyes of these rats were prepared. Although the lenses of kfrs4/kfrs4 homozygotes showed severely disorganised fibres and vacuolation, the lenses of kfrs4/+ heterozygotes appeared normal and similar to those of wild-type rats. We used positional cloning to identify the kfrs4 mutation. The mutation was mapped to an approximately 9.7-Mb region on chromosome 7, which contains the Mip gene. This gene is responsible for a dominant form of cataract in humans and mice. Sequence analysis of the mutant-derived Mip gene identified a 5-bp insertion. This insertion is predicted to inactivate the MIP protein, as it produces a frameshift that results in the synthesis of 6 novel amino acid residues and a truncated protein that lacks 136 amino acids in the C-terminal region, and no MIP immunoreactivity was observed in the lens fibre cells of kfrs4/kfrs4 homozygous rats using an antibody that recognises the C- and N-terminus of MIP. In addition, the kfrs4/+ heterozygotes showed reduced expression of Mip mRNA and MIP protein and the kfrs4/kfrs4 homozygotes showed no expression in the lens. These results indicate that the kfrs4 mutation conveys a loss-of-function, which leads to functional inactivation though the degradation of Mip mRNA by an mRNA decay mechanism. Therefore, the kfrs4 rat represents the first characterised rat model with a recessive mutation in the Mip gene. 相似文献
15.
16.
17.
18.
N. Hayashi M. Sholichin T. Sakao Y. Yamamura H. Komae 《Biochemical Systematics and Ecology》1980,8(2):109-113
A survey of chemical composition of 23 species of Asarum subgenus Heterotropa showed that the five groups could be distinguished on the basis of the presence or absence of asatone, phenol ethers and terpenes. 相似文献
19.
Vitronectin is a cell-adhesive glycoprotein in serum and plasma, also termed serum spreading factor and complement S-protein. It consists of a mixture of a polypeptide of molecular weight 75 kilodalton (kDa) and its nicked product of 65 kDa plus 10 kDa. By a quantitative immunoblotting assay, human blood samples could be classified into three distinct vitronectin types; type I (58% of the population) was 75 kDa rich and 65 kDa poor, type II (35% of the population) contained approximately equal amounts of 75 kDa and 65 kDa, and type III (5% of the population) was 75 kDa poor and 65 kDa rich. The vitronectin type did not correlate with age, sex, or ABO blood type. 相似文献
20.
Shiro Tabata Takeshi Ide Yasuyoshi Umemura Kenzo Torri 《Biochimica et Biophysica Acta (BBA)/General Subjects》1984,797(2):231-238
α-Glucosidases or maltases (EC 3.2.1.20) were purified to electrophoretic homogeneity from a respective strain of Sacchromyces cerevisiae which carries a single MAL gene, either MALα, MALβ or MALγ, using gluconate-Sepharose affinity chromography and isoelectrofocusing. Of these maltases, two types of maltase were obtained from the MALγ strain, the pI values of which were 5.6 and 5.9. From the MALα and MALβ strain was obtained only one type of maltase with the pI at 5.6 which was identical to one of the maltases from the MALγ strain. These four maltases possessed the same properties, except for pI. They were monomers with molecular weights of between 66 000 and 67 000. With regard to the substrate specificity, they hydrolyzed maltose and sucrose exclusively but not α-methulglucoside nor maltooligosaccharide. They did not differ in immunological properties. 相似文献