全文获取类型
收费全文 | 135篇 |
免费 | 13篇 |
出版年
2017年 | 1篇 |
2016年 | 2篇 |
2015年 | 3篇 |
2014年 | 6篇 |
2013年 | 8篇 |
2012年 | 7篇 |
2011年 | 6篇 |
2010年 | 4篇 |
2009年 | 2篇 |
2008年 | 1篇 |
2007年 | 5篇 |
2006年 | 3篇 |
2005年 | 4篇 |
2004年 | 6篇 |
2003年 | 6篇 |
2002年 | 4篇 |
2001年 | 4篇 |
2000年 | 1篇 |
1999年 | 4篇 |
1998年 | 5篇 |
1997年 | 3篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1987年 | 3篇 |
1986年 | 2篇 |
1985年 | 5篇 |
1984年 | 4篇 |
1983年 | 1篇 |
1982年 | 5篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1977年 | 2篇 |
1975年 | 1篇 |
1974年 | 3篇 |
1973年 | 2篇 |
1971年 | 4篇 |
1969年 | 1篇 |
1968年 | 2篇 |
1967年 | 1篇 |
1966年 | 3篇 |
1965年 | 2篇 |
1960年 | 3篇 |
1942年 | 1篇 |
1941年 | 1篇 |
1940年 | 1篇 |
1939年 | 1篇 |
排序方式: 共有148条查询结果,搜索用时 180 毫秒
141.
142.
Heinz Nika Edward Nieves David H. Hawke Ruth Hogue Angeletti 《Journal of biomolecular techniques》2013,24(3):132-153
We previously adapted the β-elimination/Michael addition chemistry to solid-phase derivatization on reversed-phase supports, and demonstrated the utility of this reaction format to prepare phosphoseryl peptides in unfractionated protein digests for mass spectrometric identification and facile phosphorylation-site determination. Here, we have expanded the use of this technique to β-N-acetylglucosamine peptides, modified at serine/threonine, phosphothreonyl peptides, and phosphoseryl/phosphothreonyl peptides, followed in sequence by proline. The consecutive β-elimination with Michael addition was adapted to optimize the solid-phase reaction conditions for throughput and completeness of derivatization. The analyte remained intact during derivatization and was recovered efficiently from the silica-based, reversed-phase support with minimal sample loss. The general use of the solid-phase approach for enzymatic dephosphorylation was demonstrated with phosphoseryl and phosphothreonyl peptides and was used as an orthogonal method to confirm the identity of phosphopeptides in proteolytic mixtures. The solid-phase approach proved highly suitable to prepare substrates from low-level amounts of protein digests for phosphorylation-site determination by chemical-targeted proteolysis. The solid-phase protocol provides for a simple, robust, and efficient tool to prepare samples for phosphopeptide identification in MALDI mass maps of unfractionated protein digests, using standard equipment available in most biological laboratories. The use of a solid-phase analytical platform is expected to be readily expanded to prepare digest from O-glycosylated- and O-sulfonated proteins for mass spectrometry-based structural characterization. 相似文献
143.
144.
D H Hawke P M Yuan K J Wilson M W Hunkapiller 《Biochemical and biophysical research communications》1987,145(3):1248-1253
Microbore HPLC methodology permits rapid and sensitive mapping of human saliva proteins. Saliva is sampled and processed in less than one hour, greatly reducing the likelihood of artifactual protein degradation. As little as 50 microliters of saliva yields proteins in sufficient quantities and purity to obtain amino terminal sequences directly. By this route we have discovered a 14 kDa protein extremely homologous to Cystatin S, but amino-terminally extended by eight amino acids. 相似文献
145.
146.
SplitsTree: analyzing and visualizing evolutionary data 总被引:15,自引:0,他引:15
MOTIVATION: Real evolutionary data often contain a number of different and
sometimes conflicting phylogenetic signals, and thus do not always clearly
support a unique tree. To address this problem, Bandelt and Dress (Adv.
Math., 92, 47-05, 1992) developed the method of split decomposition. For
ideal data, this method gives rise to a tree, whereas less ideal data are
represented by a tree-like network that may indicate evidence for different
and conflicting phylogenies. RESULTS: SplitsTree is an interactive program,
for analyzing and visualizing evolutionary data, that implements this
approach. It also supports a number of distances transformations, the
computation of parsimony splits, spectral analysis and bootstrapping.
相似文献
147.
148.