全文获取类型
收费全文 | 197篇 |
免费 | 11篇 |
专业分类
208篇 |
出版年
2021年 | 2篇 |
2019年 | 1篇 |
2018年 | 3篇 |
2017年 | 4篇 |
2016年 | 7篇 |
2015年 | 11篇 |
2014年 | 8篇 |
2013年 | 4篇 |
2012年 | 7篇 |
2011年 | 4篇 |
2010年 | 4篇 |
2009年 | 5篇 |
2008年 | 8篇 |
2007年 | 5篇 |
2006年 | 4篇 |
2005年 | 3篇 |
2004年 | 6篇 |
2003年 | 1篇 |
2002年 | 5篇 |
2001年 | 9篇 |
2000年 | 2篇 |
1999年 | 4篇 |
1998年 | 12篇 |
1997年 | 7篇 |
1996年 | 7篇 |
1995年 | 9篇 |
1994年 | 2篇 |
1993年 | 4篇 |
1992年 | 4篇 |
1991年 | 1篇 |
1990年 | 5篇 |
1989年 | 4篇 |
1988年 | 3篇 |
1987年 | 1篇 |
1985年 | 7篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1977年 | 7篇 |
1976年 | 6篇 |
1975年 | 3篇 |
1974年 | 2篇 |
1973年 | 1篇 |
1972年 | 2篇 |
1971年 | 1篇 |
排序方式: 共有208条查询结果,搜索用时 0 毫秒
91.
Water quality and algal populations in the Vaal River Barrage Reservoir have been monitored extensively for many decades, because of its importance as a water source for the most densely populated area in South Africa. Although Aulacoseira granulata (Ehrenberg) Simonsen is frequently found and at it times dominates algal assemblages in the Barrage, Aulacoseira ambigua (F.Meister) Tuji & D.M.Williams has never before been recorded at this locality. During a countrywide proficiency-testing scheme for algae counting, coordinated by Rand Water, spiral-shaped diatom colonies with distinctly curved cells were detected during May 2015. Upon investigation, it was found that the spiral colonies were Aulacoseira ambigua f. japonica, its presence in the fresh waters of South Africa being recorded for the first time. Since their first appearance during May 2015, colonies of Aulacoseira ambigua f. japonica have been constantly present in the surface waters of the Barrage. Their presence at this site can be linked to increasing eutrophication in the Vaal River, because the spiral form is known to prefer eutrophic conditions. Centric diatoms rarely form spiral colonies and there is some confusion in the literature as to the identity of this particular form, therefore in this paper we discuss this taxon, its nomenclature and ecological significance. 相似文献
92.
93.
94.
Structure and organization of the bovine beta-globin genes 总被引:1,自引:0,他引:1
Genomic clones spanning the entire cow beta-globin gene locus have been
isolated and characterized. These clones demonstrate that the linkage of
embryonic-like (epsilon) genes and pseudogenes (psi) to the previously
described fetal (gamma) and adult (beta) genes is as follows: 5'-epsilon
3-epsilon 4-psi 3-beta-epsilon 1-epsilon 2-psi 1- psi 2-gamma-3'. Present
data indicate that, like that of the goat, the fetal and adult genes arose
via block duplication of an ancestral four- gene set:
epsilon-epsilon-psi-beta. This duplication event preceded the divergence of
cows and goats, which occurred greater than or equal to 18-20 Myr ago.
However, cows do not have the additional four-gene block containing a
preadult/stress globin gene (beta C). Furthermore, the cow fetal cluster
contains an extra beta-like pseudogene, which apparently arose by a
small-scale duplication. The fixation of this duplication may indicate a
possible evolutionary role for pseudogenes.
相似文献
95.
Microsatellite allele frequencies in humans and chimpanzees, with implications for constraints on allele size 总被引:18,自引:6,他引:18
The distributions of allele sizes at eight simple-sequence repeat (SSR) or
microsatellite loci in chimpanzees are found and compared with the
distributions previously obtained from several human populations. At
several loci, the differences in average allele size between chimpanzees
and humans are sufficiently small that there might be a constraint on the
evolution of average allele size. Furthermore, a model that allows for a
bias in the mutation process shows that for some loci a weak bias can
account for the observations. Several alleles at one of the loci (Mfd 59)
were sequenced. Differences between alleles of different lengths were found
to be more complex than previously assumed. An 8-base-pair deletion was
present in the nonvariable region of the chimpanzee locus. This locus
contains a previously unrecognized repeated region, which is imperfect in
humans and perfect in chimpanzees. The apparently greater opportunity for
mutation conferred by the two perfect repeat regions in chimpanzees is
reflected in the higher variance in repeat number at Mfd 59 in chimpanzees
than in humans. These data indicate that interspecific differences in
allele length are not always attributable to simple changes in the number
of repeats.
相似文献
96.
97.
Janejira Duangjit Kent Welsh Mitchell L. Wise Borut Bohanec Michael J. Havey 《Molecular breeding : new strategies in plant improvement》2014,34(1):75-85
Higher concentrations of anthocyanins in vegetables are important for attractive appearance and may offer health benefits for consumers. The red color of onion (Allium cepa) bulbs is due primarily to the accumulation of anthocyanins. The goal of this study was to identify chromosome regions that significantly affect concentrations of anthocyanins and soluble solids in onion bulbs. Segregating haploid plants from the cross of yellow (OH1) and red (5225) inbreds were asexually propagated and bulbs were produced in replicated trials across three environments. Concentrations of soluble solids were measured at 30 days after harvest and quantitative analyses revealed a significant region on chromosome 5. Analyses using a binary model for segregation of red versus yellow bulbs revealed a significant region on chromosome 7 and two regions linked in repulsion phase on chromosome 4. These results are consistent with the complementary two-locus model previously proposed to control red versus yellow bulb colors in onion. The region on chromosome 7 mapped to the same location as the R locus, and the regions on chromosome 4 may correspond to the L and L2 loci. The intensity of red bulb color was assessed visually by a panel of evaluators and by amounts of anthocyanins [peonidin 3-glucoside and cyanidin 3-(6″-malonoyl-laminaribioside)] measured by high-performance liquid chromatography. Quantitative analyses using a normal model revealed significant quantitative trait loci on chromosomes 1, 4 and 8 affecting anthocyanin concentrations, and yellow onion contributed beneficial genetic variation to enhance red bulb color. Significant correlations were observed between these anthocyanin concentrations and panel scores, indicating that visual selection should be effective for increasing anthocyanin levels in onion bulbs. These selected populations may be more attractive to consumers, potentially provide health benefits from increased anthocyanin consumption, and be a source of natural colorants. 相似文献
98.
Animal models of spinal cord contusion injuries. 总被引:22,自引:0,他引:22
BACKGROUND AND PURPOSE: Traumatic spinal cord injury causes initial mechanical disruption of tissue, leading to a complex secondary sequence of pathophysiologic changes and neurologic impairment. These sequelae depend on the impact force delivered to the spinal cord at the time of injury. Successful clinical evaluation of the efficacy of any therapeutic regimen depends on the reliability and reproducibility of an experimental animal model. We describe a trauma device and the biomechanical parameters required to induce severe or moderate spinal cord contusion injury in cats and rats. METHODS: Recovery after injury was determined by behavioral, electrophysiologic, and histologic evaluations. RESULTS: Behavioral and electrophysiologic tests after injury clearly identified the experimental groups. A stable severe paraplegic state (defined as 6 months for cats and 8 weeks for rats), without evidence of behavioral or electrophysiologic recovery, was induced by a 65-Newton (N) load for cats and a 35-N load for rats. Moderate spinal cord contusion injury, from which cats and rats partially recovered after approximately 3 months and 4 weeks, respectively, was induced by a 45- and 25-N load, respectively. CONCLUSION: Use of these injury conditions provides reliable animal models for studies designed to evaluate potential therapeutic regimens for spinal cord injury. 相似文献
99.
M. J. Havey 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(5-6):778-782
Hybrid-onion (Allium cepa) seed is produced using systems of cytoplasmic-genic male sterility (CMS). Two different sources of CMS (S and T cytoplasms)
have been genetically characterized. Testcrosses of N-cytoplasmic maintaining and restoring genotypes to S and T cytoplasmic
lines demonstrated that different alleles, or loci, restore male fertility for these two male-sterile cytoplasms. Other sources
of CMS have been used or reported in Europe, Japan and India, and their relationships to S and T cytoplasms are not clear.
Restriction fragment length polymorphisms were identified in the organellar genomes among commercially used male-sterile cytoplasms
from Holland, Japan and India, and were compared to S and T cytoplasms. Mitochondrial DNA diversity among 58 non-S-cytoplasmic
open-pollinated onion populations was also assessed. All five putative CMS lines selected from the Indian population Nasik
White Globe were identical to S cytoplasm for all polymorphisms in the chloroplast genome, and always possessed the same-sized
mitochondrial fragments as S cytoplasm. T cytoplasm, the male-sterile cytoplasm used to produce the Dutch hybrid Hygro F1, and two sources of CMS from Japan, were similar and showed numbers of mitochondrial polymorphisms similar to those observed
among the 58 non-S-cytoplasmic open-pollinated populations. This research demonstrates that the same, or very similar, male-sterile
cytoplasms have been independently isolated and exploited for hybrid-seed production in onion.
Received: 27 October 1999 / Accepted: 12 February 2000 相似文献
100.