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101.
A report on the XXXV International Congress of Physiological Sciences, held together with Experimental Biology 2005, San Diego, USA, 31 March - 6 April 2005. 相似文献
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Jerry?SpiegelEmail author Shannon?Bennett Libby?Hattersley Mary?H.?Hayden Pattamaporn?Kittayapong Sustriayu?Nalim Daniel?Nan?Chee?Wang Emily?Zielinski-Gutiérrez Duane?Gubler 《EcoHealth》2005,2(4):273-290
This article critically examines how programs for the prevention and control of dengue fever have been conducted in the absence
of an integrated approach, and considers the social and ecological factors influencing their effectiveness. Despite recognition
of dengue fever as the most important arboviral disease affecting humans, and in spite of a greater emphasis on community-based
control approaches, the burden placed on the communities, countries, and regions affected by this disease continues to rise.
In considering historical experience in the Americas and the Asia-Pacific region, as well as the global forces that are exerting
new pressures, the important elements of successful control programs are identified as community ownership, partnership with
government, leadership, scalability, and control of immature mosquitoes. The key barriers to the exchange of knowledge and
the transdisciplinary cooperation necessary for sustainable dengue control are rooted in differences in values among policy-makers,
citizens, and scientists and are repeatedly expressed in technical, economic, cultural, geographic, and political dimensions.
Through consideration of case studies in Cuba, Guatemala, Singapore, Thailand, Indonesia, and Vietnam, the limitations of
control approaches that fail to take into account the complexities of ecological and social systems are presented. Bridges
to effective control are identified as the basis for adaptability, both of control programs to the mosquito vector’s changing
behavior and of education programs to public, regional and local particularities, as well as transdisciplinarity, community
empowerment, the ability to scale local experiences up to the macro-level, and the capacity to learn from experience to achieve
sustainability. 相似文献
104.
Mutations in the hepatocyte nuclear factor-1beta gene are associated with familial hypoplastic glomerulocystic kidney disease 总被引:3,自引:0,他引:3 下载免费PDF全文
Bingham C Bulman MP Ellard S Allen LI Lipkin GW Hoff WG Woolf AS Rizzoni G Novelli G Nicholls AJ Hattersley AT 《American journal of human genetics》2001,68(1):219-224
Familial glomerulocystic kidney disease (GCKD) is a dominantly inherited condition characterized by glomerular cysts and variable renal size and function; the molecular genetic etiology is unknown. Mutations in the gene encoding hepatocyte nuclear factor (HNF)-1beta have been associated with early-onset diabetes and nondiabetic renal disease-particularly renal cystic disease. We investigated a possible role for the HNF-1beta gene in four unrelated GCKD families and identified mutations in two families: a nonsense mutation in exon 1 (E101X) and a frameshift mutation in exon 2 (P159fsdelT). The family members with HNF-1beta gene mutations had hypoplastic GCKD and early-onset diabetes or impaired glucose tolerance. We conclude that there is genetic heterogeneity in familial GCKD and that the hypoplastic subtype is a part of the clinical spectrum of the renal cysts and diabetes syndrome that is associated with HNF-1beta mutations. 相似文献
105.
James S. McTaggart Ned Jenkinson John-Stuart Brittain Siri A. W. Greeley Andrew T. Hattersley Frances M. Ashcroft 《PloS one》2013,8(4)
Background
Gain-of-function mutations in the ATP-sensitive potassium channel can cause permanent neonatal diabetes mellitus (PNDM) or neonatal diabetes accompanied by a constellation of neurological symptoms (iDEND syndrome). Studies of a mouse model of iDEND syndrome revealed that cerebellar Purkinje cell electrical activity was impaired and that the mice exhibited poor motor coordination. In this study, we probed the hand-eye coordination of PNDM and iDEND patients using visual tracking tasks to see if poor motor coordination is also a feature of the human disease.Methods
Control participants (n = 14), patients with iDEND syndrome (n = 6 or 7), and patients with PNDM (n = 7) completed three computer-based tasks in which a moving target was tracked with a joystick-controlled cursor. Patients with PNDM and iDEND were being treated with sulphonylurea drugs at the time of testing.Results
No differences were seen between PNDM patients and controls. Patients with iDEND syndrome were significantly less accurate than controls in two of the three tasks. The greatest differences were seen when iDEND patients tracked blanked targets, i.e. when predictive tracking was required. In this task, iDEND patients incurred more discrepancy errors (p = 0.009) and more velocity errors (p = 0.009) than controls.Conclusions
These results identify impaired hand-eye coordination as a new clinical feature of iDEND. The aetiology of this feature is likely to involve cerebellar dysfunction. The data further suggest that sulphonylurea doses that control the diabetes of these patients may be insufficient to fully correct their neurological symptoms. 相似文献106.
Dynamic modification of proteins with the small ubiquitin-like modifier (SUMO) affects the stability, cellular localization, enzymatic activity, and molecular interactions of a wide spectrum of protein targets. We have developed an in vitro fluorescence-resonance-energy-transfer-based assay that uses bacterially expressed substrates for the rapid and quantitative analysis of SUMO paralog-specific C-terminal hydrolase activity. This assay has applications in SUMO protease characterization, enzyme kinetic analysis, determination of SUMO protease activity in eukaryotic cell extracts, and high-throughput inhibitor screening. In addition, while demonstrating such uses, we show that the SUMO-1 processing activity in crude HeLa cell extracts is far greater than that of SUMO-2, implying that differential maturation rates of SUMO paralogs in vivo may be functionally significant. The high degree of structural conservation across the ubiquitin-like protein superfamily suggests that the general principle of this assay should be applicable to other post-translational protein modification systems. 相似文献
107.
Maria AT Groves Lily Amanuel Jamie I Campbell D Gareth Rees Sudharsan Sridharan Donna K Finch David C Lowe Tristan J Vaughan 《MABS-AUSTIN》2014,6(1):236-245
In vitro selection technologies are an important means of affinity maturing antibodies to generate the optimal therapeutic profile for a particular disease target. Here, we describe the isolation of a parent antibody, KENB061 using phage display and solution phase selections with soluble biotinylated human IL-1R1. KENB061 was affinity matured using phage display and targeted mutagenesis of VH and VL CDR3 using NNS randomization. Affinity matured VHCDR3 and VLCDR3 library blocks were recombined and selected using phage and ribosome display protocol. A direct comparison of the phage and ribosome display antibodies generated was made to determine their functional characteristics. 相似文献
108.
Camila Carlos Mathias M Pires Nancy C Stoppe Elayse M Hachich Maria IZ Sato Tânia AT Gomes Luiz A Amaral Laura MM Ottoboni 《BMC microbiology》2010,10(1):161
Background
Escherichia coli strains are commonly found in the gut microflora of warm-blooded animals. These strains can be assigned to one of the four main phylogenetic groups, A, B1, B2 and D, which can be divided into seven subgroups (A0, A1, B1, B22, B23, D1 and D2), according to the combination of the three genetic markers chuA, yjaA and DNA fragment TspE4.C2. Distinct studies have demonstrated that these phylo-groups differ in the presence of virulence factors, ecological niches and life-history. Therefore, the aim of this work was to analyze the distribution of these E. coli phylo-groups in 94 human strains, 13 chicken strains, 50 cow strains, 16 goat strains, 39 pig strains and 29 sheep strains and to verify the potential of this analysis to investigate the source of fecal contamination. 相似文献109.
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