全文获取类型
收费全文 | 496篇 |
免费 | 22篇 |
出版年
2021年 | 3篇 |
2018年 | 5篇 |
2015年 | 12篇 |
2014年 | 6篇 |
2013年 | 26篇 |
2012年 | 24篇 |
2011年 | 16篇 |
2010年 | 15篇 |
2009年 | 14篇 |
2008年 | 28篇 |
2007年 | 15篇 |
2006年 | 19篇 |
2005年 | 24篇 |
2004年 | 13篇 |
2003年 | 8篇 |
2002年 | 21篇 |
2001年 | 16篇 |
2000年 | 24篇 |
1999年 | 14篇 |
1998年 | 5篇 |
1996年 | 3篇 |
1995年 | 9篇 |
1994年 | 3篇 |
1993年 | 6篇 |
1992年 | 18篇 |
1991年 | 12篇 |
1990年 | 11篇 |
1989年 | 9篇 |
1988年 | 10篇 |
1987年 | 8篇 |
1986年 | 6篇 |
1985年 | 9篇 |
1984年 | 4篇 |
1983年 | 4篇 |
1982年 | 8篇 |
1981年 | 5篇 |
1980年 | 3篇 |
1979年 | 7篇 |
1978年 | 8篇 |
1977年 | 8篇 |
1976年 | 10篇 |
1975年 | 6篇 |
1974年 | 3篇 |
1973年 | 6篇 |
1972年 | 2篇 |
1971年 | 5篇 |
1970年 | 9篇 |
1969年 | 3篇 |
1966年 | 3篇 |
1960年 | 2篇 |
排序方式: 共有518条查询结果,搜索用时 250 毫秒
81.
Kyoko Hatano Mikako Yamamoto Yuki Yamada Takahito Nishikata 《Phycological Research》2000,48(3):143-148
In the green alga Hydrodictyon reticulatum zoospores are arranged in a regular fashion to form an intricate hexagonal network during the asexual reproductive cycle. A monoclonal antibody which was raised against a homogenate of zoospores recognized a single poly‐peptide in zoospores with a molecular mass of 31 kDa. The antigenic polypeptide, which was designated Amy1, was localized within the cytoplasm of zoospores. The accumulation of Amy1 occurred concomitantly with the transition from multinuclear vegetative cells to mononuclear zoospores, and the degradation of Amy1 occurred concomitantly with the further development of zoospores. Amy1 was constantly expressed during the period of mononuclear zoospores. Thus, we conclude that Amy1 is a zoospore‐specific polypeptide. Using the anti‐Amy1 monoclonal antibody, we could easily distinguish between mononuclear zoospores and multinuclear vegetative net‐cells. This provides an important tool for analysing the molecular mechanisms involved in the hexagonal net formation by zoospores. 相似文献
82.
400 MHz 1H NMR of ferric low-spin cytochrome P-450scc purified from bovine adrenal cortex was measured for the first time. As compared with 1H NMR spectra of low-spin P-450cam and metMb- mercaptan complexes, paramagnetic shifts of low-spin P-450scc complexes were more divergent, suggesting that there is a subtle difference in the heme environment between P-450scc and P-450cam [1]. The paramagnetic shifts of low-spin complexes of P-450scc caused by adding nitrogenous inhibitors, aminoglutethimide and metyrapone, were different from those caused by adding an intermediate, 20α-hydroxycholesterol, and a detergent, Tween 20 [2]. The paramagnetic shifts of the metMb-mercaptan complexes were convergent compared with those of ferric low-spin P-450scc and P-450cam, suggesting that the electronic character and/or the conformation of the internal thiolate ligand in P-450scc and P-450cam are different from those of the external thiolate ligand in metMb-thiolate complexes [3]. The paramagetic shifts of the metMb-mercaptan complexes were dependent on the electron donating factor of the alkyl group of the bound mercaptans [4].Magnetic CD(MCD) spectra of ferric low-spin P-450scc, rabbit liver P-450 complexes and metMb- mercaptan complexes were also observed at various temperatures. The temperature dependences of the Soret MCD bands for the low-spin P-450 and metMb- mercaptan complexes were decidedly less pronounced than those for the low-spin metMb-CN? or imidazole complexes, suggesting that thiolate ligands markedly influence the Soret MCD band of the ferric low-spin complexes [1]. The suggestion described in [2] implied by the 1H NMR study was reconfirmed from the temperature dependence study of the Soret MCD [2]. The temperature dependences of the Soret MCD bands for low-spin P-450 complexes having a non-nitrogenous ligand were more pronounced than for those having a nitrogenous ligand. 相似文献
83.
H G Bergallo J L Luz D S Raíces F H Hatano F Martins-Hatano 《Revista brasleira de biologia》2005,65(4):583-588
The Restinga de Jurubatiba has at least 10 plant formations, including open Clusia shrubland. This formation is composed of dense shrubs of many shapes and sizes, where Clusia hilariana is one of the most important plant species. Shrublands with Clusia (CC) are poorer in plant species and less dense than shrublands without Clusia (SC). Oryzomys subflavus (Rodentia) is the most abundant small mammal species in the open Clusia shrubland. We tested the hypothesis that the abundance of rodents would increase with the size of the patch and would be higher in SC shrublands. Rodents were captured, marked and released in three 780-m-long transects. At each capture site, we evaluated the shape of the shrubland patch, calculated the area and noted the category of the shrubland. Using ANCOVA, we ascertained whether the abundance of Oryzomys subflavus increased with the sampled area and used CC and SC shrublands differently. We also verified if the size of patches used by rodents varies in the same frequency as the size of available shrublands. Rodent abundance was found to increase significantly with the area. There were no differences in the size of the patches used by rodents and the frequency of the size of available patches. This finding indicates that O. subflavus, in the study area, is a generalist species that uses its habitat according to availability. 相似文献
84.
Specific effect of Ca2+ on movement of plasmodial fragment obtained by caffeine treatment 总被引:8,自引:0,他引:8
S Hatano 《Experimental cell research》1970,61(1):199-203
85.
Shin-ichi Arimura Shin Takusagawa Shoji Hatano Mikio Nakazono Atsushi Hirai Nobuhiro Tsutsumi 《FEBS letters》1999,450(3):338-234
We have cloned a novel nuclear gene for a ribosomal protein of rice and Arabidopsis that is like the bacterial ribosomal protein S9. To determine the subcellular localization of the gene product, we fused the N-terminal region and green fluorescent protein and expressed it transiently in rice seedlings. Localized fluorescence was detectable only in chloroplasts, indicating that this nuclear gene encodes chloroplast ribosomal protein S9. The N-terminal region of rice ribosomal protein S9 was found to have a high sequence similarity to the transit peptide region of the rice chloroplast ribosomal protein L12, suggesting that these transit peptides have a common lineage. 相似文献
86.
The genus Nepenthes comprises carnivorous plants that digest insects in pitcher fluid to supplement their nitrogen uptake. In a recent study, two acid proteinases (nepenthesins I and II) were purified from the pitcher fluid. However, no other enzymes involved in prey digestion have been identified, although several enzyme activities have been reported. To identify all the proteins involved, we performed a proteomic analysis of Nepenthes pitcher fluid. The secreted proteins in pitcher fluid were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and several protein bands were detected by silver staining. The proteins were identified by in-gel tryptic digestion, de novo peptide sequencing, and homology searches against public databases. The proteins included homologues of beta-D-xylosidase, beta-1,3-glucanase, chitinase, and thaumatin-like protein, most of which are designated "pathogenesis-related proteins". These proteins presumably inhibit bacterial growth in the pitcher fluid to ensure sufficient nutrients for Nepenthes growth. 相似文献
87.
C-Glucosidic ellagitannin dimers were classified as types A-C according to a putative biogenetic oligomerization mode. They were characterized by different positions of the C-C bond between the phenolic acyl unit in one monomer and the benzylic C-1 of the open-chain glucose core in the other monomer. In recent years, four C-glucosidic tannins, melasquanins A-D (18-21), have been found in the leaves of Melaleuca squarrosa Donn ex Sm. (Myrtaceae). These are characterized as a dimer (melasquanin A) of a dimerization mode (type D), and trimers (melasquanins B-D) based on spectroscopic analysis including various two-dimensional nuclear magnetic resonance (2D NMR) experiments. Melasquanins B (19) and D (21) are C-glucosidic tannin trimers with a structure containing, non-repeating condensation modes, which was hitherto unknown. 相似文献
88.
Ken-ichi Hatano Satoshi Kikuchi Yohei Nakamura Hironobu Sakamoto Machiko Takigami Yasuyoshi Kojima 《Bioresource technology》2009,100(20):4697-4703
Molasses-based distilleries generate large volumes of a highly polluted and dark brown-colored wastewater. The present work describes the way in which an adsorbent-column chromatography can effectively remove the colorant and produce biomass ethanol from sugarcane or sugar beet molasses. It was found that the color and chemical oxygen demand of the resulting wastewater was respectively reduced by approximately 87% and 28% as compared with conventional molasses fermentation. Gas chromatography showed that the decolorized molasses maintained good ethanol productivity almost equal to that of the original molasses. Furthermore, it was revealed that the colorant concentrations of about 5 mg ml−1 in the medium were the most favorable for ethanolic fermentation. In summary, we have concluded that this method is the most effective when the adsorbent chromatography is performed just before molasses fermentation and that the decolorized molasses is an ideal substrate for fuel ethanol production. 相似文献
89.
Akihiro Nita Akinobu Matsumoto Ronghao Tang Chisa Shiraishi Kazuya Ichihara Daisuke Saito Mikita Suyama Tomoharu Yasuda Gaku Tsuji Masutaka Furue Bumpei Katayama Toshiyuki Ozawa Teruasa Murata Teruki Dainichi Kenji Kabashima Atsushi Hatano Masaki Matsumoto Keiichi I. Nakayama 《PLoS genetics》2021,17(8)
90.