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31.
Nadia Aboulaich Lamiaa Achmakh Hassan Bouziane M. Mar Trigo Marta Recio Mohamed Kadiri Baltasar Cabezudo Hassane Riadi Mohamed Kazzaz 《International journal of biometeorology》2013,57(2):197-205
Poaceae pollen is one of the most prevalent aeroallergens causing allergenic reactions. The aim of this study was to characterise the grass pollen season in Tetouan during the years 2008–2010, to analyse the effect of some meteorological parameters on the incidence of the airborne Poaceae pollen, and to establish forecasting variables for daily pollen concentrations. Aerobiological sampling was undertaken over three seasons using the volumetric method. The pollen season started in April and showed the highest pollen index in May and June, when the maximum temperature ranged from 23 to 27 °C, respectively. The annual pollen score recorded varied from year to year between 2,588 and 5,404. The main pollen season lasted 114–173 days, with peak days occurring mainly in May; the highest concentration reached 308 pollen grains/m3. Air temperature was the most important meteorological parameter and correlated positively to daily pollen concentration increase. An increase in relative humidity and precipitation was usually related to a decrease in airborne pollen content. External validation of the models performed using data from 2011 showed that Poaceae pollen concentration can be highly predicted (64.2–78.6 %) from the maximum temperature, its mean concentration for the same day in other years, and its concentration recorded on the previous day. Sensitive patients suffering allergy to Poaceae pollen are at moderate to highest risk of manifesting allergic symptoms to grass pollen over 33–42 days. The results obtained provide new information on the quantitative contribution of the Poaceae pollen to the airborne pollen of Tetouan and on its temporal distribution. Airborne pollen can be surveyed and forecast in order to warn the atopic population. 相似文献
32.
Hassan Khalil Wenxian Nie Robert A Edwards James Yoo 《Journal of visualized experiments : JoVE》2013,(80)
The myofibroblast is a stromal cell of the gastrointestinal (GI) tract that has been gaining considerable attention for its critical role in many GI functions. While several myofibroblast cell lines are commercially available to study these cells in vitro, research results from a cell line exposed to experimental cell culture conditions have inherent limitations due to the overly reductionist nature of the work. Use of primary myofibroblasts offers a great advantage in terms of confirming experimental findings identified in a cell line. Isolation of primary myofibroblasts from an animal model allows for the study of myofibroblasts under conditions that more closely mimic the disease state being studied. Isolation of primary myofibroblasts from human colon tissue provides arguably the most relevant experimental data, since the cells come directly from patients with the underlying disease. We describe a well-established technique that can be utilized to isolate primary myofibroblasts from both mouse and human colon tissue. These isolated cells have been characterized to be alpha-smooth muscle actin and vimentin-positive, and desmin-negative, consistent with subepithelial intestinal myofibroblasts. Primary myofibroblast cells can be grown in cell culture and used for experimental purposes over a limited number of passages. 相似文献
33.
Ettamimi Sara Carlier Jorge D. Cox Cymon J. Elamine Youssef Hammani Khalil Ghazal Hassan Costa Maria C. 《Extremophiles : life under extreme conditions》2019,23(6):821-834
Extremophiles - The prokaryotic communities of water bodies contaminated by acid mine drainage from the São Domingos mining area in southern Portugal were analyzed using a meta-taxonomics... 相似文献
34.
Somayeh Ghasemi Amir H. Khoshgoftarmanesh Hassan Hadadzadeh Mehran Jafari 《Journal of Plant Growth Regulation》2012,31(4):498-508
Supplying a sufficient amount of available iron (Fe) for plant growth in hydroponic nutrient solutions is a great challenge. The chelators commonly used to supply Fe in nutrient solutions have several disadvantages and may negatively affect plant growth. In this research study we have synthesized certain Fe-amino acid chelates, including Fe-arginine [Fe(Arg)2], Fe-glycine [Fe(Gly)2], and Fe-histidine [Fe(His)2], and evaluated their efficacy as an Fe source for two tomato cultivars (Lycopersicon esculentum Mill. cvs. ‘Rani’ and ‘Sarika’) grown in nutrient solution. Application of Fe-amino acid chelates significantly increased root and shoot dry matter yield of both tomato cultivars compared with Fe-EDTA. Tomato plants supplied with Fe-amino acid chelates also accumulated significantly higher levels of Fe, Zn, and N in their roots and shoots compared with those supplied with Fe-EDTA. In ‘Sarika’, the effect of Fe-amino acid chelates on shoot Fe content was in the order Fe(His)2?>?Fe(Gly)2?>?Fe(Arg)2. In ‘Rani’, the addition of all synthesized Fe-amino acid chelates significantly increased activity of ascorbate peroxidase (APX) in comparison with Fe-EDTA, whereas in ‘Sarika’, only Fe(His)2 increased shoot APX activity. The results obtained indicated that using Fe-amino acid chelates in the nutrient solution could supply a sufficient amount of Fe for plant uptake and also improve root and shoot growth of tomato plants, although this increase was cultivar-dependent. According to the results, Fe-amino acid chelates can be used as an alternative for Fe-EDTA to supply Fe in nutrient solutions. 相似文献
35.
36.
McGovern T Risse PA Tsuchiya K Hassan M Frigola G Martin JG 《American journal of physiology. Lung cellular and molecular physiology》2010,299(6):L808-L815
Airway epithelial cells release proinflammatory mediators that may contribute to airway remodeling and leukocyte recruitment. We explored the hypothesis that leukotriene D? (LTD?) may trigger the release of proremodeling factors through activation of the EGF receptor (EGFR). We particularly focused on the effects of LTD? on release of heparin-binding EGF-like factor (HB-EGF) and IL-8 (CXCL8), a potent neutrophil chemoattractant that may be released downstream of EGFR activation. To address this hypothesis, both primary (NHBE) and transformed bronchial human epithelial cells (BEAS-2B) were grown on an air-liquid interface and stimulated with LTD?. HB-EGF and CXCL8 were evaluated by ELISA in cell culture supernatants. To explore the EGFR signaling pathway, we used a broad-spectrum matrix metalloproteinase (MMP) inhibitor, GM-6001, two selective EGFR tyrosine kinase inhibitors, AG-1478 and PD-153035, an HB-EGF neutralizing antibody, and a specific small interfering RNA (siRNA) against the EGFR. Expression of the CysLT? cysteinyl leukotriene receptor was demonstrated by RT-PCR and immunocytochemistry in both BEAS-2B and NHBE cells. Four hours after stimulation with LTD?, HB-EGF and CXCL8 were significantly increased in cell culture supernatant. GM-6001 and montelukast, a specific CysLT? receptor antagonist, blocked the LTD?-induced increase in HB-EGF. All inhibitors/antagonists decreased LTD?-induced CXCL8 release. siRNA against EGFR abrogated CXCL8 release following stimulation with LTD? and exogenous HB-EGF. These findings suggest LTD? induced EGFR transactivation through the release of HB-EGF in human bronchial epithelial cells with downstream release of CXCL8. These effects may contribute to epithelial-mediated airway remodeling in asthma and other conditions associated with cysteinyl leukotriene release. 相似文献
37.
Mohsen Akbari Mohammad Hassan Fazaelipoor Ataollah Soltai Goharizi 《Biotechnology and Bioprocess Engineering》2011,16(2):407-412
Water super absorbents are low cross-linked hydrophilic polymers that absorb water in amounts up to several hundred times
their dry weight. In this study, the effect of adding these materials to the bed of a biofilter was investigated. Two equal
size biofilters were used for this purpose. One of the biofilters was packed with a mixture of perlite and a commercial polyacrylamide
based super absorbent (2.3% dry weight), and the other was packed with perlite to perform as a control. The biofilters were
inoculated with a bacterial culture that was able to grow on n-hexane as the sole source of carbon and energy. Both biofilters
removed up to 90% of the entering pollutants when using an inlet n-hexane concentration of 1 g/m3, and an air flow rate of 0.3 L/min (mass loading of 18.34 g/m3/h, and empty bed residence time of 3.27 min). The super absorbent had a positive effect on the performance of the biofilter.
While the difference in the performance of the biofilters was marginal when frequent moistening was applied, the difference
was considerable when moistening was less frequent. 相似文献
38.
Roshanida Abd Rahman Rosli Md. Illias Mohd Ghazali Mohd Nawawi Ahmad Fauzi Ismail Osman Hassan Kamarulzaman Kamaruddin 《Process Biochemistry》2004,39(12):2053-2060
Cyclodextrin glucanotransferase (CGTase) activity was observed when the bacterium was grown in the medium at various initial pH values, containing carbon, nitrogen, phosphorus and mineral salt sources at 50 °C for 24 h in the shake flasks. The optimisation of this growth medium was carried out using response surface methodology. The design contains a total of 32 experimental trials involving 10 star points and 6 replicates at the centre points. The design was employed by selecting sago starch, peptone from casein, K2HPO4, CaCl2 and initial pH as five independent variables in this study. The optimal calculated values of tested variables for maximal production of CGTase were found to be comprised of: sago starch, 16.02 g/l; peptone from casein, 20 g/l; K2HPO4, 1.4 g/l; CaCl2, 0.2 g/l and initial pH, 7.54 with a predicted CGTase activity of 14.20 U/ml. These predicted optimal parameters were tested in the laboratory and the final CGTase activity obtained was very close to the predicted value at 14.80 U/ml. 相似文献
39.
Structure and oligomerization of the PilC type IV pilus biogenesis protein from Thermus thermophilus
Type IV pili are expressed from a wide variety of Gram‐negative bacteria and play a major role in host cell adhesion and bacterial motility. PilC is one of at least a dozen different proteins that are implicated in Type IV pilus assembly in Thermus thermophilus and a member of a conserved family of integral inner membrane proteins which are components of the Type II secretion system (GspF) and the archeal flagellum. PilC/GspF family members contain repeats of a conserved helix‐rich domain of around 100 residues in length. Here, we describe the crystal structure of one of these domains, derived from the N‐terminal domain of Thermus thermophilus PilC. The N‐domain forms a dimer, adopting a six helix bundle structure with an up‐down‐up‐down‐up‐down topology. The monomers are related by a rotation of 170°, followed by a translation along the axis of the final α‐helix of approximately one helical turn. This means that the regions of contact on helices 5 and 6 in each monomer are overlapping, but different. Contact between the two monomers is mediated by a network of hydrophobic residues which are highly conserved in PilC homologs from other Gram‐negative bacteria. Site‐directed mutagenesis of residues at the dimer interface resulted in a change in oligomeric state of PilC from tetramers to dimers, providing evidence that this interface is also found in the intact membrane protein and suggesting that it is important to its function. Proteins 2010. © 2010 Wiley‐Liss, Inc. 相似文献
40.