全文获取类型
收费全文 | 248042篇 |
免费 | 22857篇 |
国内免费 | 254篇 |
出版年
2018年 | 2673篇 |
2017年 | 2609篇 |
2016年 | 3483篇 |
2015年 | 3739篇 |
2014年 | 4672篇 |
2013年 | 6681篇 |
2012年 | 7355篇 |
2011年 | 8078篇 |
2010年 | 5476篇 |
2009年 | 4916篇 |
2008年 | 7019篇 |
2007年 | 7224篇 |
2006年 | 6826篇 |
2005年 | 6544篇 |
2004年 | 6436篇 |
2003年 | 6286篇 |
2002年 | 6145篇 |
2001年 | 12074篇 |
2000年 | 12053篇 |
1999年 | 9199篇 |
1998年 | 2722篇 |
1997年 | 2776篇 |
1996年 | 2740篇 |
1995年 | 2506篇 |
1994年 | 2462篇 |
1993年 | 2351篇 |
1992年 | 7228篇 |
1991年 | 7031篇 |
1990年 | 7110篇 |
1989年 | 6890篇 |
1988年 | 6405篇 |
1987年 | 6051篇 |
1986年 | 5389篇 |
1985年 | 5703篇 |
1984年 | 4506篇 |
1983年 | 3907篇 |
1982年 | 2706篇 |
1981年 | 2545篇 |
1980年 | 2339篇 |
1979年 | 4148篇 |
1978年 | 3176篇 |
1977年 | 2925篇 |
1976年 | 2863篇 |
1975年 | 3298篇 |
1974年 | 3542篇 |
1973年 | 3558篇 |
1972年 | 3082篇 |
1971年 | 2859篇 |
1970年 | 2547篇 |
1969年 | 2322篇 |
排序方式: 共有10000条查询结果,搜索用时 599 毫秒
361.
T Tobe S Minoshima S Yamase N H Choi M Tomita N Shimizu 《Cytogenetics and cell genetics》1991,57(4):193-195
SP-40,40 is a serum glycoprotein consisting of two different subunits (alpha and beta) assembled into a dimer by disulfide bonds. Northern blot hybridization, using total RNA from several cell lines, showed that SP-40,40 is expressed in glioblastoma and testicular tumor cells, as well as hepatoma cells. Spot blot hybridization of flow-sorted human chromosomes, using a SP-40,40 cDNA fragment as a probe, localized the gene for SP-40,40 to human chromosome 8. This gene has been given the designation CLI, for complement lysis inhibitor, by the Human Gene Nomenclature Committee. 相似文献
362.
363.
The biting midge Culicoides sonorensis Wirth and Jones (Diptera: Ceratopogonidae) transmits pathogens to both livestock and wildlife. Biting midge surveillance relies heavily on light traps for collection; however, little is known about the light spectra preferences of C. sonorensis midges. A light assay arena was constructed and light‐emitting diodes (LEDs) of various light spectra were used as light sources to evaluate midge photoattraction. A comparison of responses to light spectra indicated the highest proportions of C. sonorensis were attracted to ultraviolet (UV) light and that midges differentiated 10‐nm differences in wavelength. Stronger intensities of UV light resulted in greater attraction. Midges exhibited both sugar‐seeking and escape behaviours under different conditions of sugar supplementation before and during the experiment. These behaviours occurred with lights of 355 nm and 365 nm in wavelength. Based on the results of this study, the attraction of C. sonorensis to light traps can be improved through the use of bright LEDs at 355 nm or 365 nm. 相似文献
364.
365.
366.
367.
368.
The binding of norepinephrine (NE) to plasma proteins of fresh human blood obtained from healthy volunteers was studied by ultrafiltration at different NE concentrations and incubation times at 37 degrees C. At 1.7 nM L-[3H]-NE binding was approximately 25%. The binding was rapid and was not influenced by the incubation time. [3H]-NE could be dissociated from its binding sites by acid precipitation and, after HPLC, showed to be unchanged NE. No difference in NE binding was found between plasma collected in EGTA-GSH or heparin solution. There was no degradation of NE when incubated in plasma at 37 degrees C for 10 h, even without the addition of antioxidants. Therefore, in the present study, binding represented interaction of unchanged NE with plasma proteins. The whole plasma binding was saturable over the range of 0.66 nM to 0.59 mM of NE. Scatchard plot of specific binding revealed high-affinity sites with a Kd of 5.4 nM and a Bmax of 3.9 fmoles.mg-1 protein, and low-affinity sites with a Kd of 2.7 microM and a Bmax of 3.3 pmoles.mg-1 protein. Electrophoretic characterization of NE-binding proteins showed that about 60% of bound NE was associated to albumin, and 20% to prealbumin. NE binding to pure human plasma proteins was also studied using ultrafiltration. Scatchard analyses revealed a single class of very high-affinity binding sites for prealbumin (Kd 4.9 nM), a single class of binding sites for alpha 1-acid glycoprotein (Kd 54 microM) and two classes of binding sites for albumin with high (Kd 1.7 microM) and low (Kd 0.8 mM) affinities respectively. The main results obtained in this study - a) reversibility of NE binding, b) stability of free and bound NE in plasma, c) involvement of the prealbumin as a specific binding protein - point out to a specific transport for NE in human blood plasma. 相似文献
369.
370.