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51.
52.
Yasuo Kitagawa Etsuro Sugimoto Hideo Chiba 《Bioscience, biotechnology, and biochemistry》2013,77(1):199-206
The amino acid composition of beef liver d-glycerate dehydrogenase (EC 1.1.1.29) was determined. Results of sodium dodecyl sulfate gel electrophoresis and measurements of the number of NADH bound by the enzyme and the number of the essential sulfhydryl groups suggested that the enzyme was composed of two identical subunits with the molecular weight of 36,000. Close relation between the essential sulfhydryl groups and the coenzyme binding site was also suggested. Effect of an alkylating agent (bromopyruvate) with the structure similar to the substrate was studied. Effects of iodoacetate and iodoacetamide were also studied. It was suggested that these reagents behaved as active-site-directed irreversible inhibitors of the enzyme. Bromopyruvate exhibited a high affinity to the enzyme. Iodoacetate (anionic reagent) had a higher affinity than iodoacetamide (neutral reagent). 相似文献
53.
Masaaki Yoshikawa Etsuro Sugimoto Hideo Chiba 《Bioscience, biotechnology, and biochemistry》2013,77(5):1005-1013
It was indicated from ultraviolet difference spectra and ultracentrifugal experiments that associations occurred between two casein components (αs- and κ-caseins, β- and κ-caseins and αs- and β-caseins) at lower CaCl2 concentrations (2~3 mm) and that aromatic amino acid residues participated in the associations. Chemical modification studies with 2-hydroxy-5-nitrobenzylbromide indicated that tryptophane residues of each casein component were not essential for these associations. It was also demonstrated by nitration of tyrosine residues with tetranitromethane that tyrosine residues of κ-casein were essential for αs·κ-association and for β·κ-association and that tyrosine residues of αs-casein were important to αs·β-association.Interactions between casein components were also studied at higher CaCl2 concentration (10 mm) which is enough for micelle formation. It was found that tyrosine residues of κ- casein played an important role for the stabilization of αs- and β-caseins. Properties of the nitrated-β-casein were almost the same as that of the native β-casein except the absorption spectrum. αs·β-Interaction in the presence of 10 mm CaCl2 was investigated by use of the nitrated-β-casein instead of the native β-casein. It was proved that αs-casein was stabilized by the nitrated-β-casein and that precipitation of the nitrated-β-casein increased in the presence of αs-casein.The mechanism of interactions between casein components at higher CaCl2 concentration (10 mm) are discussed in connection with the associations at lower CaCl2 concentrations (2~3 mm). 相似文献
54.
55.
The plant‐specific CDKB1‐CYCB1 complex mediates homologous recombination repair in Arabidopsis 下载免费PDF全文
56.
Morita Y Araki H Sugimoto T Takeuchi K Yamane T Maeda T Yamamoto Y Nishi K Asano M Shirahama-Noda K Nishimura M Uzu T Hara-Nishimura I Koya D Kashiwagi A Ohkubo I 《FEBS letters》2007,581(7):1417-1424
Legumain/asparaginyl endopeptidase (EC 3.4.22.34) is a novel cysteine protease that is abundantly expressed in the late endosomes and lysosomes of renal proximal tubular cells. Recently, emerging evidence has indicated that legumain might play an important role in control of extracellular matrix turnover in various pathological conditions such as tumor growth/metastasis and progression of atherosclerosis. We initially found that purified legumain can directly degrade fibronectin, one of the main components of the extracellular matrix, in vitro. Therefore, we examined the effect of legumain on fibronectin degradation in cultured mouse renal proximal tubular cells. Fibronectin processing can be inhibited by chloroquine, an inhibitor of lysosomal degradation, and can be enhanced by the overexpression of legumain, indicating that fibronectin degradation occurs in the presence of legumain in lysosomes from renal proximal tubular cells. Furthermore, in legumain-deficient mice, unilateral ureteral obstruction (UUO)-induced renal interstitial protein accumulation of fibronectin and renal interstitial fibrosis were markedly enhanced. These findings indicate that legumain might have an important role in extracellular matrix remodeling via the degradation of fibronectin in renal proximal tubular cells. 相似文献
57.
Prostaglandin E receptors 总被引:14,自引:0,他引:14
Prostaglandin (PG) E(2) exerts its actions by acting on a group of G-protein-coupled receptors (GPCRs). There are four GPCRs responding to PGE(2) designated subtypes EP1, EP2, EP3, and EP4 and multiple splicing isoforms of the subtype EP3. The EP subtypes exhibit differences in signal transduction, tissue localization, and regulation of expression. This molecular and biochemical heterogeneity of PGE receptors leads to PGE(2) being the most versatile prostanoid. Studies on knock-out mice deficient in each EP subtype have defined PGE(2) actions mediated by each subtype and identified the role each EP subtype plays in various physiological and pathophysiological responses. Here we review recent advances in PGE receptor research. 相似文献
58.
Yuna Sugimoto Michiko Murohashi Satoko Arakawa Shinya Honda Shigeomi Shimizu 《Biochemical and biophysical research communications》2019,508(2):480-486
In chemical biology, the elucidation of chemical target is crucial for successful drug development. Because MHC class I molecules present peptides from intracellular damaged proteins, it might be possible to identify targets of a chemical by analyzing peptide sequences on MHC class I. Therefore, we treated cells with the autophagy-inducing chemical TMD-457 and identified the peptides presented on MHC class I. Many of the peptides were derived from molecules involved in ER trafficking and ER stress, which were confirmed by morphological and biochemical analyses. Therefore, our results demonstrate that analyzing MHC class I peptides is useful for the detection of chemical targets. 相似文献
59.
Sakamoto Kazunori Ogiwara Natsuko Kaji Tomomitsu Sugimoto Yurie Ueno Mitsuru Sonoda Masatoshi Matsui Akihiro Ishida Junko Tanaka Maho Totoki Yasushi Shinozaki Kazuo Seki Motoaki 《Journal of plant research》2019,132(4):541-568
Journal of Plant Research - Soybean (Glycine max) roots establish associations with nodule-inducing rhizobia and arbuscular mycorrhizal (AM) fungi. Both rhizobia and AM fungi have been shown to... 相似文献
60.
Functional and Physical Interaction between Rad24 and Rfc5 in the Yeast Checkpoint Pathways 总被引:9,自引:8,他引:9 下载免费PDF全文
Toshiyasu Shimomura Seiko Ando Kunihiro Matsumoto Katsunori Sugimoto 《Molecular and cellular biology》1998,18(9):5485-5491
The RFC5 gene encodes a small subunit of replication factor C (RFC) complex in Saccharomyces cerevisiae and has been shown to be required for the checkpoints which respond to replication block and DNA damage. Here we describe the isolation of RAD24, known to play a role in the DNA damage checkpoint, as a dosage-dependent suppressor of rfc5-1. RAD24 overexpression suppresses the sensitivity of rfc5-1 cells to DNA-damaging agents and the defect in DNA damage-induced Rad53 phosphorylation. Rad24, like Rfc5, is required for the regulation of Rad53 phosphorylation in response to DNA damage. The Rad24 protein, which is structurally related to the RFC subunits, interacts physically with RFC subunits Rfc2 and Rfc5 and cosediments with Rfc5. Although the rad24Δ mutation alone does not cause a defect in the replication block checkpoint, it does enhance the defect in rfc5-1 mutants. Furthermore, overexpression of RAD24 suppresses the rfc5-1 defect in the replication block checkpoint. Taken together, our results demonstrate a physical and functional interaction between Rad24 and Rfc5 in the checkpoint pathways. 相似文献