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61.
A. Kaga T. Ishii K. Tsukimoto E. Tokoro O. Kamijima 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(2):207-213
A genetic linkage map was developed with 86 F2 plants derived from an interspecific cross between azuki bean (Vigna angularis, 2n=2x=22) and rice bean (V. umbellata, 2n=2x=22). In total, 14 linkage groups, each containing more than 4 markers, were constructed with one phenotypic, 114 RFLP
and 74 RAPD markers. The total map size was 1702 cM, and the average distance between markers was 9.7 cM. The loci showing
significant deviation from the expected ratio clustered in several linkage groups. Most of the skewed loci were due to the
predominance of rice bean alleles. The azuki-rice bean linkage map was compared with other available maps of Vigna species in subgenus Ceratotropis. Based on the lineage of the common mapped markers, 7 and 16 conserved linkage blocks were found in the interspecific map
of azuki bean ×V. nakashimae and mungbean map, respectively. Although the present map is not fully saturated, it may facilitate gene tagging, QTL mapping
and further useful gene transfer for azuki bean breeding.
Received: 20 March 1999 / Accepted: 29 April 1999 相似文献
62.
Unique Induction of CA1 LTP Components After Intake of Theanine, an Amino Acid in Tea Leaves and its Effect on Stress Response 总被引:1,自引:0,他引:1
Takeda A Tamano H Suzuki M Sakamoto K Oku N Yokogoshi H 《Cellular and molecular neurobiology》2012,32(1):41-48
Theanine, γ-glutamylethylamide, is one of the major amino acid components in green tea. This study was undertaken to evaluate
the effect of theanine intake on long-term potentiation (LTP) induction at hippocampal CA1 synapses and exposure to acute
stress. Young rats were fed water containing 0.3% theanine after birth. Key findings: Serum corticosterone level was markedly
decreased by theanine intake. Because this decrease can modify synaptic plasticity, the effect of theanine intake was examined
focused on CA1 LTP induction. CA1 LTP induced by a 100-Hz tetanus for 1 s was almost the same extent in hippocampal slices
from theanine-administered rats, whereas that induced by a 200-Hz tetanus for 1 s was significantly attenuated. 2-Amino-5-phosphonovalerate
(APV), an N-methyl-d-aspartate (NMDA) receptor antagonist, significantly attenuated CA1 LTP induced by a 200-Hz tetanus in the control rats, but
not in theanine-administered rats. Interestingly, APV completely blocked CA1 LTP induced by a 100-Hz tetanus in the control
rats, while scarcely blocking it in theanine-administered rats. These results indicate that theanine intake reduces NMDA receptor-dependent
CA1 LTP, while increasing NMDA receptor-independent CA1 LTP. Furthermore, neither 100-Hz tetanus-induced LTP nor 200-Hz tetanus-induced
LTP was attenuated in theanine-administered rats after exposure to tail suspension stress, suggesting that the lack of NMDA
receptor-dependent CA1 LTP by theanine intake is involved in ameliorating the attenuation of CA1 LTP after tail suspension.
This study is the first to indicate that theanine intake modifies the mechanism of CA1 LTP induction. 相似文献
63.
Zinc is a trace nutrient for the brain and a signal factor to serve for brain function. A portion of zinc is released from glutamatergic (zincergic) neuron terminals in the brain. Synaptic Zn2+ signaling is involved in synaptic plasticity such as long-term potentiaion (LTP), which is a cellular mechanism of memory. The block and/or loss of synaptic Zn2+ signaling in the hippocampus and amygdala with Zn2+ chelators affect cognition, while the role of synaptic Zn2+ signal is poorly understood, because zinc-binding proteins are great in number and multi-functional. Chronic zinc deficiency also affects cognition and cognitive decline induced by zinc deficiency might be associated with the increase in plasma glucocorticoid rather than the decrease in synaptic Zn2+ signaling. On the other hand, excess glutamatergic (zincergic) neuron activity induces excess influx of extracellular Zn2+ into hippocampal neurons, followed by cognitive decline. Intracellular Zn2+ dynamics, which is linked to presynaptic glutamate release, is critical for LTP and cognitive performance. This paper deals with insight into cognition from zinc as a nutrient and signal factor. 相似文献
64.
Zhenghua Li Gang Zhao Jingling Shen Kimi Araki Kyoko Haruna Seiya Inoue Jun Wang Ken-ichi Yamamura 《Transgenic research》2011,20(1):191-200
To produce a humanized mouse, it is critical to obtain a correct expression of a human gene/cDNA after insertion into a mouse
locus. We previously generated a targeted allele in which the PGK-neo cassette, flanked by lox71 and loxP, was inserted into
the first exon of the mouse endogenous transthyretin (Ttr) gene in ES cells. Using these ES cells, we showed that a human
transthyretin (TTR) cDNA with the PGK-puro cassette can be efficiently inserted into this locus by Cre-mediated recombination,
and that the human TTR cDNA was expressed in a tissue-specific manner under the control of the mouse endogenous Ttr promoter.
To examine whether the PGK-puro cassette or IRES could affect the expression of human TTR cDNA, we generated four mouse lines
using Cre and Flp-mediated recombination. The mouse line containing the PGK-puro cassette, but not IRES, exhibited quantitatively
and temporally similar expression of human TTR cDNA. Removal of the PGK-puro cassette significantly downregulated the expression
of the cDNA. The insertion of IRES sequence upstream of the human TTR cDNA resulted in decreased expression, even in the presence
of the PGK-puro cassette. The mouse line containing IRES, but not PGK-puro, showed the lowest level of expression. These results
suggest that the PGK-puro cassette is necessary to obtain the enhanced expression of a co-existing human cDNA in the mouse
Ttr locus, even though the expression of co-existing cDNA was under the control of the mouse endogenous promoter. 相似文献
65.
Photo‐identification matches of humpback whales (Megaptera novaeangliae) from feeding areas in Russian Far East seas and breeding grounds in the North Pacific 下载免费PDF全文
Olga V. Titova Olga A. Filatova Ivan D. Fedutin Ekaterina N. Ovsyanikova Haruna Okabe Nozomi Kobayashi Jo Marie V Acebes Alexandr M. Burdin Erich Hoyt 《Marine Mammal Science》2018,34(1):100-112
Humpback whales migrate seasonally from high latitude feeding areas to lower latitude breeding areas for mating and calving. In 2004–2006, a North Pacific basin‐wide study called SPLASH was conducted as an international collaboration among various groups of researchers. The Russian Far East consists of multiple high latitude feeding areas and during SPLASH, 102 whales were identified and compared to catalogs from breeding areas. Our goal in this study was to further investigate the migratory destinations of whales from the Russian Far East using a total of 1,459 photographs of whales identified from 2004 to 2014. We compared the latest Russian catalog with the SPLASH catalog from wintering areas (2004–2006) and with two additional regional catalogs from Okinawa (1989–2006) and the northern Philippines (2000–2006). We found a total of 152 matches: 106 with Asian, 35 with Hawaiian, and 11 with Mexican breeding grounds. The match rate was higher in mainland Kamchatka and consisted mostly of whales from the Asian breeding ground. In the Commander Islands, the proportion of whales from Asia was twice that of Hawaii and six times higher than Mexico. The total match rate was low, supporting the hypothesis of some undiscovered humpback whale breeding location in the North Pacific. 相似文献
66.
67.
Diel vertical migration (DVM) and diel feeding rhythm of two cladocerans, Daphnia longispina and Bosmina coregoni were investigated at the pelagic area of Lake Toya (Hokkaido, Japan) in May, August and October 1992. Both species performed nocturnal DVM. The amplitude of DVM, however, became smaller from May to October. Such seasonal variations in DVM could not be explained by light penetration and/or water temperature. The two species had a clear feeding rhythm; they fed at night in May and October but also after sunrise in August. These feeding rhythms appeared to be related to the light-dark cycle, but were not necessarily associated with their DVM. We suggest that the diel feeding rhythm and DVM are regulated independently by light cues. 相似文献
68.
Ishizaki Y Fukuoka H Ishizaki T Katsura T Nishimura Y Haruna M Suzuki Y Kawakubo K Gunji A 《Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology》1997,4(1):S95-S98
We investigated the changes of the psychological state during 20 days bed rest (BR) in 10 young participants,(5 males and 5 females, age; 19-24 yrs). Psychological assessment was accomplished by the following authorized 3 indices: 1) Zung's Self-rating Depression Scale, 2) Cornell Medical Index, and 3) Goldberg's General Health Questionnaire. Zung's Self-rating Depression Scale is a indicator for depression, and the other two indices were used for detecting signs of neurosis. The results from Zung's Self-rating Depression Scale and the General Health Questionnaire in this study indicated a tendency toward depression and neurosis developing during BR, respectively. The scores in the Cornell Medical Index did not change. Urinary excretion of 17-hydroxycorticosteroid increased significantly, indicating that BR immobilization induced an important psychological stress. We were unable to determine which factors caused the changes in psychological state during BR, immobilization insert or personal problems. 相似文献
69.
Fukuoka H Nishimura Y Haruna M Suzuki Y Oyama K Igawa S Sekiguchi C Murai T Shigematsu T Sudoh M Akimura H Gunji A 《Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology》1997,4(1):S75-S81
Immobilization induces abnormal bone metabolism and severe decalcification of bone. To investigate the effect of middle-term immobilization on bone metabolism, we studied 10 young healthy males and females during bed rest for 20 days. Bone mineral density (BMD) rapidly decreased in both lumbar and metacarpal bones. No bone showed consistent BMD alterations, partial increase and partial decrease, and both lumbar and metacarpal bone showed similar rapid BMD change. Urinary excretion of pyridinoline tended to slightly increase by day 10, and to decline by day 20 (mean +/-SE: 34.2 +/-7.4, 26.3+/-4.6 nmol day-1, respectively). Neither alkaline phosphatase (isoform III) nor tartrate-resistant acid phosphatase, changed, suggesting that in the early stage of immobilization bone matrix in some part might increase or be resorbed without any activation of osteoblast or osteoclast, resulting in rapid calcification or decalcification, respectively. 相似文献
70.
Masayuki Shiba Takao Kato Moritoshi Funasako Eisaku Nakane Shoichi Miyamoto Toshiaki Izumi Tetsuya Haruna Moriaki Inoko 《PloS one》2016,11(2)
BackgroundPsoriasis vulgaris is a chronic inflammatory skin disease with an immune-genetic background. It has been reported as an independent risk factor for coronary heart disease (CHD) in the United States and Europe. The purpose of this study was to investigate the association between psoriasis and CHD in a hospital-based population in Japan.MethodsFor 113,065 in-hospital and clinic patients at our institution between January 1, 2011 and January 1, 2013, the diagnostic International Classification of Diseases (ICD)-10 codes for CHD, hypertension, dyslipidemia, diabetes, and psoriasis vulgaris were extracted using the medical accounting system and electronic medical record, and were analyzed.ResultsThe prevalence of CHD (n = 5,167, 4.5%), hypertension (n = 16,476, 14.5%), dyslipidemia (n = 9,236, 8.1%), diabetes mellitus (n = 11,555, 10.2%), and psoriasis vulgaris (n = 1,197, 1.1%) were identified. The prevalence of CHD in patients with hypertension, dyslipidemia, diabetes, and psoriasis vulgaris were 21.3%, 22.2%, 21.1%, and 9.0%, respectively. In 1,197 psoriasis patients, those with CHD were older, more likely to be male, and had more number of the diseases surveyed by ICD-10 codes. Multivariate analysis showed that psoriasis vulgaris was an independent associated factor for CHD (adjusted odds ratio [OR]: 1.27; 95% confidence interval [CI]: 1.01–1.58; p = 0.0404) along with hypertension (adjusted OR: 7.78; 95% CI: 7.25–8.36; p < 0.0001), dyslipidemia (adjusted OR: 2.35; 95% CI: 2.19–2.52; p < 0.0001), and diabetes (adjusted OR: 2.86; 95% CI: 2.67–3.06; p < 0.0001).ConclusionPsoriasis vulgaris was independently associated with CHD in a hospital-based population in Japan. 相似文献