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排序方式: 共有319条查询结果,搜索用时 15 毫秒
311.
Daranagama Nayani Dhanushka Shioya Koki Yuki Masahiro Sato Haruna Ohtaki Yuki Suzuki Yoshiyuki Shida Yosuke Ogasawara Wataru 《Journal of industrial microbiology & biotechnology》2019,46(6):831-842
Journal of Industrial Microbiology & Biotechnology - Filamentous fungi produce a variety of proteases with significant biotechnological potential and show diverse substrate specificities.... 相似文献
312.
Hiroki Kobayashi Haruna Nishimura Norio Kudo Hiroyuki Osada 《Bioscience, biotechnology, and biochemistry》2020,84(10):2113-2120
ABSTRACT Small molecules that regulate cell stemness have the potential to make a major contribution to regenerative medicine. In the course of screening for small molecules that affect stemness in mouse embryonic stem cells (mESCs), we discovered that NPD13432, an aurone derivative, promoted self-renewal of mESCs. Normally, mESCs start to differentiate upon withdrawal of 2i/LIF. However, cells treated with the compound continued to express endogenous Nanog, a pluripotency marker protein essential for sustaining the undifferentiated state, even in the absence of 2i/LIF. Biochemical characterization revealed that NPD13432 inhibited GSK3α and GSK3β with IC50 values of 92 nM and 310 nM, respectively, suggesting that the compound promotes self-renewal in mESCs by inhibiting GSK3. The chemical structure of the compound is unique among known molecules with this activity, providing an opportunity to develop new inhibitors of GSK3, as well as chemical tools for investigating cell stemness. 相似文献
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Takumi Itoh Ryo Hatano Yoshiya Horimoto Taketo Yamada Dan Song Haruna Otsuka Yuki Shirakawa Shuji Mastuoka Noriaki Iwao Thomas M. Aune Nam H. Dang Yutaro Kaneko Ko Okumura Chikao Morimoto Kei Ohnuma 《Cell death & disease》2021,12(6)
Triple-negative breast cancer (TNBC) has a poor prognosis compared to other breast cancer subtypes. Although epidermal growth factor receptor (EGFR) is overexpressed in TNBC, clinical trials with EGFR inhibitors including tyrosine kinase inhibitors (EGFR-TKI) in TNBC have heretofore been unsuccessful. To develop effective EGFR-targeted therapy for TNBC, the precise mechanisms of EGFR-TKI resistance in TNBC need to be elucidated. In this study, to understand the molecular mechanisms involved in the differences in EGFR-TKI efficacy on TNBC between human and mouse, we focused on the effect of IL-26, which is absent in mice. In vitro analysis showed that IL-26 activated AKT and JNK signaling of bypass pathway of EGFR-TKI in both murine and human TNBC cells. We next investigated the mechanisms involved in IL-26-mediated EGFR-TKI resistance in TNBC. We identified EphA3 as a novel functional receptor for IL-26 in TNBC. IL-26 induced dephosphorylation and downmodulation of EphA3 in TNBC, which resulted in increased phosphorylation of AKT and JNK against EGFR-TKI-induced endoplasmic reticulum (ER) stress, leading to tumor growth. Meanwhile, the blockade of IL-26 overcame EGFR-TKI resistance in TNBC. Since the gene encoding IL-26 is absent in mice, we utilized human IL-26 transgenic (hIL-26Tg) mice as a tumor-bearing murine model to characterize the role of IL-26 in the differential effect of EGFR-TKI in human and mice and to confirm our in vitro findings. Our findings indicate that IL-26 activates the bypass pathway of EGFR-TKI, while blockade of IL-26 overcomes EGFR-TKI resistance in TNBC via enhancement of ER stress signaling. Our work provides novel insights into the mechanisms of EGFR-TKI resistance in TNBC via interaction of IL-26 with its newly identified receptor EphA3, while also suggesting IL-26 as a possible therapeutic target in TNBC.Subject terms: Stress signalling, Cell death and immune response, Breast cancer 相似文献
316.
Much less attention has been paid to Zn2+ in artificial cerebrospinal fluid (ACSF), i.e., extracellular medium, used for in vitro slice experiments than divalent cations such as Ca2+. Approximately 2 mM Ca2+ is added to conventional ACSF from essentiality of Ca2+ signaling in neurons and glial cells. However, no Zn2+ is added to it, even though the importance of Zn2+ signaling in them is recognizing. On the other hand, synaptic Zn2+ homeostasis is changed during brain slice preparation. Therefore, it is possible that not only neuronal excitation but also synaptic plasticity such as long-term potentiation is modified in ACSF without Zn2+, in which original physiology might not appear. The basal (static) levels of intracellular (cytosolic) Zn2+ and Ca2+ are not significantly different between brain slices prepared with conventional ACSF without Zn2+ and pretreated with ACSF containing 20 nM ZnCl2 for 1 h. In the case of mossy fiber excitation, however, presynaptic activity assessed with FM 4–64 is significantly suppressed in the stratum lucidum of brain slices pretreated with ACSF containing Zn2+, indicating that hippocampal excitability is enhanced in brain slices prepared with ACSF without Zn2+. The evidence suggests that low nanomolar concentration of Zn2+ is necessary for ACSF. Furthermore, exogenous Zn2+ has opposite effect on LTP induction between in vitro and in vivo experiments. It is required to pay attention to extracellular Zn2+ concentration to understand synaptic function precisely. 相似文献
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Fujimoto Keisaku Ura Midori Yamazaki Haruna Uematsu Akikazu 《Sleep and biological rhythms》2018,16(3):323-330
Sleep and Biological Rhythms - The purpose was to determine whether the instability of parasympathetic nerve (PN) function is associated with fragmentation of sleep and the instability can be... 相似文献
319.
Mohammed Haruna Jaiswal Sanjay K. Mohammed Mustapha Mbah Glory C. Dakora Felix D. 《Physiology and Molecular Biology of Plants》2020,26(6):1263-1280
Physiology and Molecular Biology of Plants - With legumes, symbiotic N2 fixation can meet the species N demand and reduce the over-reliance on chemical fertilizers in tropical regions where N... 相似文献