全文获取类型
收费全文 | 591篇 |
免费 | 16篇 |
出版年
2023年 | 1篇 |
2022年 | 4篇 |
2021年 | 10篇 |
2020年 | 1篇 |
2019年 | 3篇 |
2018年 | 9篇 |
2017年 | 5篇 |
2016年 | 10篇 |
2015年 | 14篇 |
2014年 | 24篇 |
2013年 | 40篇 |
2012年 | 29篇 |
2011年 | 53篇 |
2010年 | 19篇 |
2009年 | 24篇 |
2008年 | 27篇 |
2007年 | 48篇 |
2006年 | 33篇 |
2005年 | 38篇 |
2004年 | 37篇 |
2003年 | 33篇 |
2002年 | 19篇 |
2001年 | 18篇 |
2000年 | 15篇 |
1999年 | 6篇 |
1998年 | 6篇 |
1997年 | 3篇 |
1996年 | 7篇 |
1995年 | 6篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 5篇 |
1991年 | 5篇 |
1990年 | 8篇 |
1989年 | 4篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1986年 | 6篇 |
1985年 | 4篇 |
1984年 | 3篇 |
1983年 | 1篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1974年 | 1篇 |
1973年 | 2篇 |
排序方式: 共有607条查询结果,搜索用时 15 毫秒
561.
562.
Ryo Watanabe Haruki Mizoguchi Hideaki Oikawa Hirofumi Ohashi Koichi Watashi Hiroki Oguri 《Bioorganic & medicinal chemistry》2017,25(11):2851-2855
Densely functionalized tetrahydropyridines were stereoselectively synthesized from 1,6-dihydropyridines. Exploiting a carbonyl group installed at the C3 position of the 1,6-dihydropyridine system, we devised a strategy for cyanomethylation at C2/C6 and subsequent divergent installation of an allyl group at C3/C5 in a highly regio- and stereo-controlled manner. This versatile protocol for programmable functionalization of the 1,6-dihydropyridine system allows the divergent and streamlined synthesis of multiply-substituted tetrahydropyridines as an important class of biologically and medicinally relevant scaffolds. Two of the N-heterocyclic compounds bearing an alkyl nitrile group showed anti-hepatitis C virus (HCV) activity. 相似文献
563.
Yoshiaki Shirata Asami Wakasa Kiyoshi Miura Hironori Nakamura Yoshinobu Matsumoto 《Bioscience, biotechnology, and biochemistry》2017,81(9):1829-1836
We examined a methylcellulose (MC) formulation that gels at body temperature for enteral alimentation. Betaine was found to have a lowering effect on the gelation temperature of the MC solution. The thermal gelation temperature of a body heat-responsive (BHR) gelling MC formulation, consisting of 2% MC, 15% glucose, 1.2% sodium citrate, and 3.5% betaine mixture, was approximately 32 °C, indicating that it could gel in response to body heat. Glucose release from the BHR gels was delayed at 37 °C in an in vitro study. In rats, oral administration of BHR gelling MC formulation delayed an increase in blood glucose and appearance of 13CO2 in expired air in a 13C-acetate breath test in comparison with the control. These results suggested that the BHR gelling MC formulation was gelled in the stomach and delayed gastric emptying after oral administration and glucose in the gels was absorbed slowly. 相似文献
564.
Masakiyo Sakaguchi Masami Watanabe Rie Kinoshita Haruki Kaku Hideo Ueki Junichiro Futami Hitoshi Murata Yusuke Inoue Shun-Ai Li Peng Huang Endy Widya Putranto I. Made Winarsa Ruma Yasutomo Nasu Hiromi Kumon Nam-ho Huh 《Molecular biotechnology》2014,56(7):621-630
For expression of genes in mammalian cells, various vectors have been developed using promoters including CMV, EF-1α, and CAG promoters and have been widely used. However, such expression vectors sometimes fail to attain sufficient expression levels depending on the nature of cargo genes and/or on host cell types. In the present study, we aimed to develop a potent promoter system that enables high expression levels of cargo genes ubiquitously in many different cell types. We found that insertion of an additional promoter downstream of a cargo gene greatly enhanced the expression levels. Among the constructs we tested, C-TSC cassette (C: CMV-RU5′ located upstream; TSC: another promoter unit composed of triple tandem promoters, hTERT, SV40, and CMV, located downstream of the cDNA plus a polyadenylation signal) had the most potent capability, showing far higher efficiency than that of potent conventional vector systems. The results indicate that the new expression system is useful for production of recombinant proteins in mammalian cells and for application as a gene therapeutic measure. 相似文献
565.
566.
Rice (Oryza sativa L.) is the major staple food consumed by half of the world's population. Rice seeds have gained recent attention as bioreactors for the production of human pharmaceuticals such as therapeutic proteins or peptides. Rice seed production platforms have many advantages over animal cell or microbe systems in terms of cost-effectiveness, scalability, safety, product stability and productivity. Rice seed-based human pharmaceuticals are expected to become innovative therapies as edible drugs. Therapeutic proteins can be sequestered within natural cellular compartments in rice seeds and protected from harsh gastrointestinal environments. This review presents the state-of-the-art on the construction of gene cassettes for accumulation of pharmaceutical proteins or peptides in rice seeds, the generation of transgenic rice plants, and challenges involved in the use of rice seeds to produce human pharmaceuticals. 相似文献
567.
Kumiko Kawaguchi-Nagata Takehiko Watanabe Kazutaka Maeyama Atsushi Yamatodani Haruki Okamura Toshihide Tamura Ko Shoji Yukihiko Kitamura 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》1988,972(3)
When cells of mouse myelomonocytyc leukemia cell line, WEHI-3B, were cultured in the presence of actinomycin D plus the serum which was obtained from mice injected with bacterial endotoxin, i.e., lipopolysaccharide, their histidine decarboxylase (l-histidine carboxy-lyase, EC 4.1.1.22) (HDC) activity increased about 100-fold with a peak at 48 h. According to the increase in HDC activity, the expression of surface antigens associated with macrophages, such as Mac II, Mac III and Ia, increased markedly on WEHI-3B cells as well as their morphological changes to macrophages. Histamine levels in the culture medium increased concomitantly with the increase in the HDC activity in WEHI-3B cells, whereas the histamine contents inside the cells did not increase remarkably. Furthermore, the addition of lipopolysaccharide to the culture medium caused an additional 2-fold increase in the HDC activity of WEHI-3B cells. These results indicate that the increase in HDC activity in WEHI-3B cells may represent an event in the process of the differentiation to macrophages. 相似文献
568.
The envelope membrane was isolated from intact spinach chloroplastsby gentle osmotic treatment in a medium containing appropriateamounts of cations to prevent dissociation and fragmentationof the thylakoids. This treatment allowed us to separate effectivelythe envelope membranes from the thylakoids with one-step (0.6M/0.9 M) sucrose density gradient centrifugation. The envelope membrane contained both glyceroglycolipids andglycerophospholipids, as does the thylakoid membrane. Therewere, however, notable differences in the relative amounts oflipid components between these two membranes. The major glyceroglycolipidin the envelope membrane was digalactosyl diglyceride, whereasmonogalactosyl diglyceride was the major one in the thylakoid.The envelope membrane was characterized by a high content ofglycerophospholipids, as much as three-fold that in the thylakoidmembrane. Phosphatidyl choline, which is known to be minor inthe thylakoids and abundant in the microsomal and mitochondrialmembranes, was a major component, accounting for 50% of thetotal glycerophospholipids. The dual character of lipid compositionof the envelope membrane is discussed in terms of its chemicaland structural connection to the other intracellular membranesystems. (Received May 26, 1975; ) 相似文献
569.
Haruki Yamada Iwao Suzuki Yoshio Kumazawa Yasuhiro Kawamura Kimifusa Mizunoe Yukihiko Aramaki Toshio Miyazaki 《Biochimica et Biophysica Acta (BBA)/General Subjects》1978,538(3):627-630
Cell surface and extracellular polysaccharide fractions obtained from Dictyostelium discoideum NC-4 cultured in bacteria-free medium showed strong B-cell mitogenic activities. Upon periodate treatment of the extracellular polysaccharide fraction this activity completely disappeared. The extracellular polysaccharide fraction could also enhance the antibody response in vitro against sheep red blood cells. 相似文献
570.
Hirokazu Kawagishi Hiroshi Suzuki Haruki Watanabe Hiroko Nakamura Takehiko Sekiguchi Takeomi Murata Taichi Usui Kimio Sugiyama Hiroyuki Suganuma Takahiro Inakuma Kiyoshi Ito Yohichi Hashimoto Mayumi Ohnishi-Kameyama Tadahiro Nagata 《Biochimica et Biophysica Acta (BBA)/General Subjects》2000,1474(3):299-308
In an experiment in which rats were allowed free access to food and water, the rats did not eat the diet containing a mushroom Pleurotus ostreatus even if they were emaciated. A P. ostreatus lectin (POL) was isolated from the mushroom as the food intake-suppression principle. In hemagglutination inhibition assays, Me-αGalNAc was the most potent inhibitor among the monosaccharides tested. Among all the sugars tested, 2′-fucosyllactose (Fucα1→2Galβ1→4Glc) was the strongest inhibitor and its inhibitory potency was five times greater than that of Me-αGalNAc. POL exhibited a binding ability to bovine submaxillary mucin (BSM) and asialo-BSM and the other glycoproteins were inert to the binding. The food intake-suppressing activity of POL was dependent on the dose. The diet containing 0.1% POL caused a 50% decrease in the food intake of rats against the control. 相似文献