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121.
Koichi Kobayashi Takafumi Narise Kintake Sonoike Haruki Hashimoto Naoki Sato Maki Kondo Mikio Nishimura Mayuko Sato Kiminori Toyooka Keiko Sugimoto Hajime Wada Tatsuru Masuda Hiroyuki Ohta 《The Plant journal : for cell and molecular biology》2013,73(2):250-261
The galactolipids monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) are the predominant lipids in thylakoid membranes and indispensable for photosynthesis. Among the three isoforms that catalyze MGDG synthesis in Arabidopsis thaliana, MGD1 is responsible for most galactolipid synthesis in chloroplasts, whereas MGD2 and MGD3 are required for DGDG accumulation during phosphate (Pi) starvation. A null mutant of Arabidopsis MGD1 (mgd1‐2), which lacks both galactolipids and shows a severe defect in chloroplast biogenesis under nutrient‐sufficient conditions, accumulated large amounts of DGDG, with a strong induction of MGD2/3 expression, during Pi starvation. In plastids of Pi‐starved mgd1‐2 leaves, biogenesis of thylakoid‐like internal membranes, occasionally associated with invagination of the inner envelope, was observed, together with chlorophyll accumulation. Moreover, the mutant accumulated photosynthetic membrane proteins upon Pi starvation, indicating a compensation for MGD1 deficiency by Pi stress‐induced galactolipid biosynthesis. However, photosynthetic activity in the mutant was still abolished, and light‐harvesting/photosystem core complexes were improperly formed, suggesting a requirement for MGDG for proper assembly of these complexes. During Pi starvation, distribution of plastid nucleoids changed concomitantly with internal membrane biogenesis in the mgd1‐2 mutant. Moreover, the reduced expression of nuclear‐ and plastid‐encoded photosynthetic genes observed in the mgd1‐2 mutant under Pi‐sufficient conditions was restored after Pi starvation. In contrast, Pi starvation had no such positive effects in mutants lacking chlorophyll biosynthesis. These observations demonstrate that galactolipid biosynthesis and subsequent membrane biogenesis inside the plastid strongly influence nucleoid distribution and the expression of both plastid‐ and nuclear‐encoded photosynthetic genes, independently of photosynthesis. 相似文献
122.
Shinji Masuda Rei Ikeda Tatsuru Masuda Haruki Hashimoto Tohru Tsuchiya Hiroko Kojima Jiro Nomata Yuichi Fujita Mamoru Mimuro Hiroyuki Ohta Ken‐ichiro Takamiya 《The Plant journal : for cell and molecular biology》2009,58(6):952-960
In angiosperms, chlorophyll biosynthesis is light dependent. A key factor in this process is protochlorophyllide oxidoreductase (POR), which requires light to catalyze the reduction of protochlorophyllide to chlorophyllide. It is believed that this protein originated from an ancient cyanobacterial enzyme that was introduced into proto‐plant cells during the primary symbiosis. Here we report that PORs from the cyanobacteria Gloeobacter violaceus PCC7421 and Synechocystis sp. PCC6803 function in plastids. First, we found that the G. violaceus POR shows a higher affinity to its substrate protochlorophyllide than the Synechocystis POR but a similar affinity to plant PORs. Secondly, the reduced size of prolamellar bodies caused by a knockdown mutation of one of the POR genes, PORA, in Arabidopsis could be complemented by heterologous expression of the cyanobacterial PORs. Photoactive protochlorophyllide in the etioplasts of the complementing lines, however, was retained at a low level as in the parent PORA knockdown mutant, indicating that the observed formation of prolamellar bodies was irrelevant to the assembly of photoactive protochlorophyllide. This work reveals a new view on the formation of prolamellar bodies and provides new clues about the function of POR in the etioplast–chloroplast transition. 相似文献
123.
Singh RP Kashiwamura S Rao P Okamura H Mukherjee A Chauhan VS 《Journal of immunology (Baltimore, Md. : 1950)》2002,168(9):4674-4681
A possible protective role of IL-18 in host defense against blood-stage murine malarial infection was studied in BALB/c mice using a nonlethal strain, Plasmodium yoelii 265, and a lethal strain, Plasmodium berghei ANKA. Infection induced an increase in mRNA expression of IL-18, IL-12p40, IFN-gamma, and TNF-alpha in the case of P. yoelii 265 and an increase of IL-18, IL-12p40, and IFN-gamma in the case of P. berghei ANKA. The timing of mRNA expression of IL-18 in both cases was consistent with a role in the induction of IFN-gamma protein expression. Histological examination of spleen and liver tissues from infected controls treated with PBS showed poor cellular inflammatory reaction, massive necrosis, a large number of infected parasitized RBCs, and severe deposition of hemozoin pigment. In contrast, IL-18-treated infected mice showed massive infiltration of inflammatory cells consisting of mononuclear cells and Kupffer cells, decreased necrosis, and decreased deposition of the pigment hemozoin. Treatment with rIL-18 increased serum IFN-gamma levels in mice infected with both parasites, delayed onset of parasitemia, conferred a protective effect, and thus increased survival rate of infected mice. Administration of neutralizing anti-IL-18 Ab exacerbated infection, impaired host resistance and shortened the mean survival of mice infected with P. berghei ANKA. Furthermore, IL-18 knockout mice were more susceptible to P. berghei ANKA than were wild-type C57BL/6 mice. These data suggest that IL-18 plays a protective role in host defense by enhancing IFN-gamma production during blood-stage infection by murine malaria. 相似文献
124.
125.
Atsushi Ishihara Fumio Matsuda Hisashi Miyagawa Kyo Wakasa 《Metabolomics : Official journal of the Metabolomic Society》2007,3(3):319-334
Advances in molecular breeding technologies have enabled manipulation of the concentrations of specific plant components by
modifying the genes that play a key role in their production. This has provided new opportunities to enhance the nutritional
quality of major crops. However, given that metabolic pathways form a highly integrated network, any alteration in a given
biosynthetic pathway is most likely to effect secondary and unpredicted changes in the metabolite profile of other pathways.
Metabolomics technologies can contribute to the efficient detection of such unexpected effects caused by genetic modification.
This has relevance not only from the perspective of safety evaluations of newly developed crops, but to basic science focused
on uncovering hitherto unknown regulatory mechanisms associated with the biosynthesis and catabolism of primary and secondary
metabolites in plants. In this review, recent advances in plant metabolic engineering for the overproduction of tryptophan
(Trp), one of the essential amino acids, are described. In particular, the efficacy of a transgene OASA1D that encodes a mutant anthranilate synthase (AS) α subunit of rice in specifically elevating levels of Trp without marked
secondary effects on the metabolite profile of rice is demonstrated. Related topics, such as regulation of Trp biosynthesis,
possible interactions between the biosyntheses of Trp and other aromatic amino acids, and translocation of Trp in are discussed
based on findings derived from metabolomic analyses of Trp-overproducing transgenic plants. 相似文献
126.
In order to understand a role of the Ca(2+) ion on the structure and function of a Ca(2+)-dependent family I.3 lipase from Pseudomonas sp. MIS38, apo-PML, holo-PML, holo-PML*, and the N-terminal domain alone (N-fragment) were prepared and biochemically characterized. Apo-PML and holo-PML represent refolded proteins in the absence and presence of the Ca(2+) ion, respectively. Holo-PML* represents a holo-PML dialyzed against 20 mM Tris-HCl (pH 7.5). The results suggest that the C-terminal domain of PML is almost fully unfolded in the apo-form and its folding is induced by Ca(2+) binding. The folding of this C-terminal domain may be required to make a conformation of the N-terminal catalytic domain functional. 相似文献
127.
128.
First evidence of Wolbachia infection in populations of grasshopper Podisma sapporensis (Orthoptera: Acrididae)
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Alexander G. Bugrov Yury Yu. Ilinsky Anton Strunov Mariya Zhukova Elena Kiseleva Shin‐ichi Akimoto Haruki Tatsuta 《Entomological Science》2016,19(3):296-300
The brachypterous grasshopper Podisma sapporensis (Orthoptera: Acrididae) is distributed throughout the Sakhalin, Kunashir and Hokkaido Islands. Karyotypes of this species consist of two major chromosomal races with different sex chromosome systems, XO/XX and XY/XX. Molecular phylogeographic analysis of the chromosome races and subraces confirms the genetic divergence of the races and subraces in P. sapporensis. Here we first report that P. sapporensis is infected with Wolbachia consisting of three variants on wsp locus, while gatB locus was monomorphic. Furthermore, observation of cell tissue of P. sapporensis using electron microscopy confirmed the infection of Wolbachia that was inferred from polymerase chain reaction and revealed the distribution of the bacteria in the head, thorax and abdomen of P. sapporensis embryos. Our finding may shed new light on Wolbachia as a possible agent causing hybrid dysfunction resulting from experimental crosses between chromosome races or subraces of P. sapporensis. 相似文献
129.
Young giant water bug nymphs prefer larger prey: changes in foraging behaviour with nymphal growth in Kirkaldyia deyrolli
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Shin‐Ya Ohba Haruki Tatsuta 《Biological journal of the Linnean Society. Linnean Society of London》2016,117(3):601-606
Raptorial characteristics may evolve in predators because of their importance in obtaining food. The giant water bug, Kirkaldyia deyrolli, possesses a claw on the terminal segment of the raptorial foreleg that is crucial for capturing prey. Claw curvature has been previously shown to change during growth in this species, but the adaptive significance of this change has not yet been explored. Predation experiments have demonstrated that young nymphs with highly curved claws caught proportionally larger prey than older nymphs with less‐curved claws. Catching behaviours for a certain prey size differed significantly between young and older nymphs. The observation that nymphal growth affects prey‐catching behaviour in the giant water bug supports the hypothesis that predators can change catching behaviours based on changes in raptorial characteristics in order to maximize prey resources acquired at each developmental stage. 相似文献
130.
Dr. Masatoshi Ito Hideo Tsuda Katsuhiko Oguro Kozo Mutoh Hideyuki Shiraishi Yukiyoshi Shirasaka Haruki Mikawa 《Neurochemical research》1990,15(9):933-936
GABA-gated chloride ion influx was measured in brain microsac preparations of epileptic El mice. There was significantly greater sensitivity to GABA in stimulated El mice (which had 14–18 convulsions induced at weekly intervals) than in unstimulated El mice (which had not experienced convulsions) or ddY mice. GABA-gated chloride ion influx was significantly decreased 20 min after a single convulsion, and returned to the preconvulsion level 60 min after a convulsion. These findings suggest that the functional state of GABA-gated chloride channel in El mice is changed secondarily by single or repeated convulsions. 相似文献