On the patterns of abundance and diversity of macrolichens of Chopta-Tunganath in the Garhwal Himalaya

A total of 3211 colonies of macrolichens, from twelve 50 m × 10 m plots distributed across four macrohabitat (vegetation) types between 1500 m–3700 m in the Chopta-Tunganath landscape of the Garhwal Himalaya, yielded 13 families with 15 genera and 85 species.Lobaria retigera stood out as a broad-niched generalist species with moderate levels of abundance in all the three major microhabitats, viz. rock, soil and wood across 83% of all the plots sampled, whereasUmbilicaria indica emerged as an abundantly occurring specialist confined to rock substrates.Heterodermia incana andLeptogium javanicum appeared to be rare members of the community as they were encountered only once during the field survey. Woody microhabitats turned out to be richer than rock and soil substrates for macrolichens. Amongst the macrohabitats, middle altitude (2500–2800 m)Quercus forest was richest in species and genera followed by high altitude (2900–3200 m)Rhododendron forest, higher altitude grasslands (3300–3700 m) and then the lower elevation (1500 m)Quercus forest. Species, genus and family level alphaas well as beta-diversities were significantly correlated with each other, implying that higher taxonomic ranks such as genera may be used as surrogates for species thus facilitating cost- and time-effective periodic monitoring of the biodiversity of macrolichens. Dynamics of the diversity of lichen communities in relation to various forms of environmental disturbance including livestock grazing and tourism as dominant land use activities in the higher Himalaya need further research.     

Non-timber forest products (NTFPs): a viable option for biodiversity conservation and livelihood enhancement in central Himalaya

The present study aims to document detail information of some of the selected wild edible having enormous potential for livelihood enhancement and socio-economic development by making a variety of value added products. To this end, some of the wild edibles of central Himalaya were selected and prioritized for harnessing their economic potential along with their detail information in terms of distribution, ethnobiology, phenophases and appropriate time of harvesting so as to make communities well aware about the resource availability and their harvesting period round the year. The cost-benefit analysis of each value added product prepared from selected wild edibles was worked out in detail and these analyses revealed that total monetary output, as well as the net return, is very high for all value added products prepared. Since wild edible fruits or other edible parts can be collected from wild free of cost except labour is involved in collection of these wild edibles bio-resources. In addition, information on a participatory action research framework & approaches for promoting participatory conservation of these wild edible species were also highlighted for appropriate management of these resources. The present attempt provides a practical example of sustainable utilization of wild edibles, their potential in livelihood improvement of local people, distribution and phenophases and availability in natural conditions, participatory conservation of these wild edibles may help policy planners at the regional and national levels to link livelihood/socio-economic development with conservation.     

Understanding enzyme behavior in a crowded scenario through modulation in activity,conformation and dynamics

Macromolecular crowding, inside the physiological interior, modulates the energy landscape of biological macromolecules in multiple ways. Amongst these, enzymes occupy a special place and hence understanding the function of the same in the crowded interior is of utmost importance. In this study, we have investigated the manner in which the multidomain enzyme, AK3L1 (PDB ID: 1ZD8), an isoform of adenylate kinase, has its features affected in presence of commonly used crowders (PEG 8, Dextran 40, Dextran 70, and Ficoll 70). Michaelis Menten plots reveal that the crowders in general enhance the activity of the enzyme, with the K_m and V_max values showing significant variations. Ficoll 70, induced the maximum activity for AK3L1 at 100 g/L, beyond which the activity reduced. Ensemble FRET studies were performed to provide insights into the relative domain (LID and CORE) displacements in presence of the crowders. Solvation studies reveal that the protein matrix surrounding the probe CPM (7-diethylamino-3-(4-maleimido-phenyl)-4-methylcoumarin) gets restricted in presence of the crowders, with Ficoll 70 providing the maximum rigidity, the same being linked to the decrease in the activity of the enzyme. Through our multipronged approach, we have observed a distinct correlation between domain displacement, enzyme activity and associated dynamics. Thus, keeping in mind the complex nature of enzyme activity and the surrounding bath of dense soup that the biological entity remains immersed in, indeed more such approaches need to be undertaken to have a better grasp of the “enzymes in the crowd”.     

CERTAIN EMBRYOLOGICAL AND BIOCHEMICAL ASPECTS OF CYTOKININ SD 8339 IN CONVERTING SEX OF A MALE VITIS VINIFERA (SYLVESTRIS)

Certain embryological and biochemical effects of the cytokinin (SD 8339) in converting flower sex from male to hermaphrodite were studied in a clone of Vitis vinifera L. (sylvestris). The cytokinin accelerated the meiotic division of the megaspore mother cell, mitotic divisions of the megaspore and cells of pistillate tissue, and increased the rate of protein synthesis in flower buds. Two working hypotheses for the possible mode of action of the cytokinin in sex conversion are presented.     

Phenological features in relation to growth forms and biomass accumulation in an alpine meadow of the Central Himalaya

Phenological records of 50 plant species were made during 1988–89 in an alpine meadow (30° 10-30° 13 N lat. and 79° 39-79°-41 E long.) of Central Himalaya located between 3100–3750 m elevation. The growth initiation occurred when temperature began to rise continuously and the resulting in snowmelt. The peaks of the various phenophases succeeded one after another in time, within a period of about four months (from May to September) which is longer than the period reported for the alpine sites of higher latitudes. The period of growth initiation appeared to be related to growth form, the species showing earlier growth initiation (when temperatures were lower) deployed leaves to lower heights, close to the ground. A majority of forbs completed growth cycle earlier than the grasses. For example, one group represented by Trachydium roylei reached peak growth a few weeks before did another group of species, represented by Danthonia cachemyriana, which is indicative of niche separation of the respective group of species. Activities of accumulation of live and dead shoot biomass were clearly separated in time in most communities, the former occurring from May to August, and the latter mainly in September. Nomenclature: Osmaston (1926).     

The Transmembrane Region of Guard Cell SLAC1 Channels Perceives CO2 Signals via an ABA-Independent Pathway in Arabidopsis

The guard cell S-type anion channel, SLOW ANION CHANNEL1 (SLAC1), a key component in the control of stomatal movements, is activated in response to CO₂ and abscisic acid (ABA). Several amino acids existing in the N-terminal region of SLAC1 are involved in regulating its activity via phosphorylation in the ABA response. However, little is known about sites involved in CO₂ signal perception. To dissect sites that are necessary for the stomatal CO₂ response, we performed slac1 complementation experiments using transgenic plants expressing truncated SLAC1 proteins. Measurements of gas exchange and stomatal apertures in the truncated transgenic lines in response to CO₂ and ABA revealed that sites involved in the stomatal CO₂ response exist in the transmembrane region and do not require the SLAC1 N and C termini. CO₂ and ABA regulation of S-type anion channel activity in guard cells of the transgenic lines confirmed these results. In vivo site-directed mutagenesis experiments targeted to amino acids within the transmembrane region of SLAC1 raise the possibility that two tyrosine residues exposed on the membrane are involved in the stomatal CO₂ response.