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61.
Effect of experimental nitrogen load on methane and nitrous oxide fluxes on ombrotrophic boreal peatland 总被引:8,自引:0,他引:8
Nykänen Hannu Vasander Harri Huttunen Jari T. Martikainen Pertti J. 《Plant and Soil》2002,242(1):147-155
Methane (CH4) and nitrous oxide (N2O) dynamics were studied in a boreal Sphagnum fuscum pine bog receiving annually (from 1991 to 1996) 30 or 100 kg NH4NO3-N ha–1. The gas emissions were measured during the last three growing seasons of the experiment. Nitrogen treatment did not affect the CH4 fluxes in the microsites where S. fuscum and S. angustifolium dominated. However, addition of 100 kg NH4NO3-N ha–1 yr–1 increased the CH4 emission from those microsites dominated by S. fuscum. This increase was associated with the increase in coverage of cotton grass (Eriophorum vaginatum) induced by the nitrogen treatment. The differences in the CH4 emissions were not related to the CH4 oxidation and production potentials in the peat profiles. The N2O fluxes were negligible from all microsites. Only minor short-term increases occurred after the nitrogen addition. 相似文献
62.
Mikkola Satu Mikhailov Sergey N. Efimtseva Ekaterina Neuvonen Kari Oivanen Mikko Beigelman Leonid Lönnberg Harri 《Origins of life and evolution of the biosphere》2002,32(4):303-310
Stereoisomeric uridylyl(3',5')uridines D,L-UpU andL,D-UpU were synthesised. Their cleavage was followed in thepresence of acid, base and metal ion catalysts to studywhether the stereochemistry affects the inherent reactivity ofthe internucleosidic phosphodiester bond, and whether the lowmolecular weight catalysts can distinguish between thesubstrates. The rate constants obtained were compared to thoseof D,D-UpU. The comparison shows that the stability of thephosphodiester bond does not depend on the stereochemistry ofthe sugar rings. In contrast slight reactivity differences areobserved in the presence of metal ion catalysts, whichsuggests that selective cleavage of stereoisomeric substrateseven by small molecular weight chemical catalysts may bepossible. 相似文献
63.
Jorma Arpalahti Rainer Käppi Pejtti Lehikoinen Harri Lönnberg 《Nucleosides, nucleotides & nucleic acids》2013,32(3):447-448
Abstract Combined UV- and liquid scintillation-HPLC has been applied to study the complexing of purine nucleosides with Pt(II)-diamine ions, and the effect of the complex formation on the acidic depurination. 相似文献
64.
Supplementary feeding of ungulates, being widely used in game management, may have unwanted consequences. Its role in agricultural damage is well-studied, but few studies have considered the potential for the practice to attract ground nest predators. Our goal was to identify the factors influencing ground nest predation in the vicinity of year-round supplementary feeding sites for wild boar and to characterise their spatiotemporal scope. We conducted two separate artificial ground nest experiments in five different hunting districts in south-eastern Estonia. The quantity of food provided and distance of a nest from the feeding site were the most important factors determining predation risk. Larger quantities of food resulted in higher predation risk, while predation risk responded in a non-linear fashion to distance from the feeding site. Although predation risk eventually decreases if supplementary feeding is ceased for at least four years, recently abandoned feeding sites still pose a high predation risk. 相似文献
65.
66.
Standing genetic diversity and selection at functional gene loci are associated with differential invasion success in two non‐native fish species 下载免费PDF全文
Kyle W. Wellband Harri Pettitt‐Wade Aaron T. Fisk Daniel D. Heath 《Molecular ecology》2018,27(7):1572-1585
Invasive species are expected to experience a unique combination of high genetic drift due to demographic factors while also experiencing strong selective pressures. The paradigm that reduced genetic diversity should limit the evolutionary potential of invasive species, and thus, their potential for range expansion has received little empirical support, possibly due to the choice of genetic markers. Our goal was to test for effects of genetic drift and selection at functional genetic markers as they relate to the invasion success of two paired invasive goby species, one widespread (successful) and one with limited range expansion (less successful). We genotyped fish using two marker types: single nucleotide polymorphisms (SNPs) in known‐function, protein‐coding genes and microsatellites to contrast the effects of neutral genetic processes. We identified reduced allelic variation in the invaded range for the less successful tubenose goby. SNPs putatively under selection were responsible for the observed differences in population structure between marker types for round goby (successful) but not tubenose goby (less successful). A higher proportion of functional loci experienced divergent selection for round goby, suggesting increased evolutionary potential in invaded ranges may be associated with round goby's greater invasion success. Genes involved in thermal tolerance were divergent for round goby populations but not tubenose goby, consistent with the hypothesis that invasion success for fish in temperate regions is influenced by capacity for thermal tolerance. Our results highlight the need to incorporate functional genetic markers in studies to better assess evolutionary potential for the improved conservation and management of species. 相似文献
67.
van Leeuwen Wessel M. A. Sallinen Mikael Virkkala Jussi Lindholm Harri Hirvonen Ari Hublin Christer Porkka-Heiskanen Tarja Härmä Mikko 《Sleep and biological rhythms》2018,16(1):45-54
Sleep and Biological Rhythms - Sleep restriction is increasingly common and associated with the development of health problems. We investigated how the neuroendocrine stress systems respond to... 相似文献
68.
Syed Bilal Ahmad Andrabi Subhash Kumar Tripathi Obaiah Dirasantha Kartiek Kanduri Sini Rautio Catharina C. Gross Sari Lehtimäki Kanchan Bala Johanna Tuomisto Urvashi Bhatia Deepankar Chakroborty Laura L. Elo Harri Lähdesmäki Heinz Wiendl Omid Rasool Riitta Lahesmaa 《Cell reports》2018,22(8):2094-2106
69.
Emmanuela Daza Secco Jari Haimi Harri Högmander Sara Taskinen Jenni Niku Kristian Meissner 《Wetlands Ecology and Management》2018,26(4):597-611
As most ecosystems, peatlands have been heavily exploited for different human purposes. For example, in Finland the majority is under forestry, agriculture or peat mining use. Peatlands play an important role in carbon storage, water cycle, and are a unique habitat for rare organisms. Such properties highlight their environmental importance and the need for their restoration. To monitor the success of peatland restoration sensitive indicators are needed. Here we test whether testate amoebae can be used as a reliable bioindicator for assessing peatland condition. To qualify as reliable indicators, responses in testate amoebae community structure to ecological changes must be stronger than random spatial and temporal variation. In this study, we simultaneously assessed differences between the effects of seasonality, intermediate scale spatial variation and land uses on living testate amoebae assemblages in natural, forested and restored peatlands. We expected the effects of seasonality on testate amoebae communities to be less pronounced than those of land use and within site variation. On average, natural sites harboured the highest richness and density, while the lowest numbers were found at forestry sites. Despite small changes observed in taxa dominance and differences in TA community structure between seasons and years at some sites, spatial heterogeneity, temperature, pH, nor water table depth seemed to significantly affect testate amoebae communities. Instead, observed differences were related to type of land use, which explained 75% of the community variation. Our results showed that testate amoebae community monitoring is a useful tool to evaluate impacts of human land use on boreal peatlands. 相似文献
70.
Miikka Pakkala Anu Jylhsalmi Ping Wu Jari Leinonen Ulf‐Hkan Stenman Harri Santa Jouko Vepslinen Mikael Perkyl Ale Nrvnen 《Journal of peptide science》2004,10(7):439-447
Prostate-specific antigen (PSA), a member of the kallikrein sub-group of the trypsin serine protease family, is a widely used marker for prostate cancer. Several sequences with specific binding to PSA have been identified by using phage display peptide libraries. The GST-fusion proteins of the characterized sequences have been shown to increase the enzyme activity of PSA to a synthetic substrate. The corresponding three cyclic synthetic analogues CVFTSNYAFC (A-1), CVFAHNYNYLVC (B-2) and CVAYCIEHHCWTC (C-4) have similar PSA promoting activity. Despite differences in the amino acid sequences, all three peptides bind to the same region of PSA. The conformation of the peptides was investigated by proton NMR spectroscopy. In addition, alanine replacement was used to characterize the prerequisites for binding. It is proposed that interactions with PSA are based on the aromatic and hydrophobic features of the amino acid side chains. Furthermore, it is suggested that peptides form beta-turn structures forced by cysteine bridges directing important aromatic side chains to the same side of the turn-structure. 相似文献