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排序方式: 共有155条查询结果,搜索用时 15 毫秒
21.
Preparation of Agglutinating Antisera and Fluorescent-Antibody Conjugates Against Pasteurella tularensis in Equines 总被引:1,自引:0,他引:1
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James H. Green Richard C. Bolin Russell K. Carver Herman Gross Nan Pigott William K. Harrell 《Applied microbiology》1970,19(6):894-897
The serological response in burros and horses to the viable LVS strain of Pasteurella tularensis was studied. High-titered agglutinating antisera and fluorescent-antibody conjugates were obtained in both groups of animals. Maximum titers were obtained in horses 14 to 21 days after the start of vaccination and in burros 21 to 28 days after the start of vaccination. The use of Woodhour's adjuvants or booster inoculations did not result in increased titers. 相似文献
22.
23.
Jason W. Skudlarek Christina N. DiMarco Kerim Babaoglu Anthony J. Roecker Joseph G. Bruno Mark A. Pausch Julie A. OBrien Tamara D. Cabalu Joanne Stevens Joseph Brunner Pamela L. Tannenbaum W. Peter Wuelfing Susan L. Garson Steven V. Fox Alan T. Savitz Charles M. Harrell Anthony L. Gotter Christopher J. Winrow Paul J. Coleman 《Bioorganic & medicinal chemistry letters》2017,27(6):1364-1370
In an ongoing effort to explore the use of orexin receptor antagonists for the treatment of insomnia, dual orexin receptor antagonists (DORAs) were structurally modified, resulting in compounds selective for the OX2R subtype and culminating in the discovery of 23, a highly potent, OX2R-selective molecule that exhibited a promising in vivo profile. Further structural modification led to an unexpected restoration of OX1R antagonism. Herein, these changes are discussed and a rationale for selectivity based on computational modeling is proposed. 相似文献
24.
Using historical data from the Utah Population Database, this analysis finds significant, consistent, but small adverse mortality
effects for mothers after age 50 who had mostly sons. Examination of age-dependent effects indicates that this association
increases with mother’s age. Additionally, mothers who had mostly daughters faced mortality risks that increased with age.
Offspring sex composition did not have a significant effect on paternal mortality. Interaction analyses were conducted to
examine the effect of offspring sex composition with regard to historical period, residential location, socioeconomic status,
and childhood survival. No other interactions were found to be statistically significant. Having mostly boys remained detrimental
to maternal mortality regardless of childhood survival.
相似文献
Ken R. SmithEmail: |
25.
Clark RL Arima A Makori N Nakata Y Bernard F Gristwood W Harrell A White TE Wier PJ 《Birth defects research. Part B, Developmental and reproductive toxicology》2008,83(4):418-434
BACKGROUND: The developmental toxicity, toxicokinetics, and hematological effects of the antimalarial drug, artesunate, were previously studied in rats and rabbits and have now been studied in cynomolgus monkeys. METHODS: Groups of up to 15 pregnant females were dosed on Gestation Days (GD) 20–50 or for 3–7‐day intervals. RESULTS: At 30 mg/kg/day, 6 embryos died between GD30 and GD40. Histologic examination of 3 live embryos (GD26–GD36) revealed a marked reduction in embryonic erythroblasts and cardiomyopathy. At 12 mg/kg/day, 6 embryos died between GD30 and GD45. Four surviving fetuses examined on GD100 had no malformations, but long bone lengths were slightly decreased. At the developmental no‐adverse‐effect‐level (4 mg/kg/day), maternal plasma AUC was 3.68 ng.h/mL for artesunate and 6.93 ng.h/ml for its active metabolite, dihydroartemisinin (DHA). No developmental toxicity occurred with administration of 12 mg/kg/day for 3 or 7 days, GD29–31 or GD27–33 (maternal plasma AUC of 9.84 ng.h/mL artesunate and 16.4 ng.h/mL DHA). Exposures at embryotoxic doses were substantially lower than human therapeutic exposures. However, differences in monkey and human Vss for artesunate (0.5 L/kg vs. 0.18 L/kg) confound relying solely on AUC for assessing human risk. Decreases in reticulocyte count occur at therapeutic doses in humans. Changes to reticulocyte counts at embryotoxic doses in monkeys (≥12 mg/kg/day) were variable and generally minor. CONCLUSIONS: Artesunate was embryolethal at ≥12 mg/kg/day when dosed for at least 12 days at the beginning of organogenesis, but not when dosed for 3 or 7 days, indicating that developmental toxicity of artesunate is dependent upon duration of dosing in cynomologus monkeys. Birth Defects Res (Part B) 83:418–434, 2008. © 2008 Wiley‐Liss, Inc. 相似文献
26.
Bergman JM Roecker AJ Mercer SP Bednar RA Reiss DR Ransom RW Meacham Harrell C Pettibone DJ Lemaire W Murphy KL Li C Prueksaritanont T Winrow CJ Renger JJ Koblan KS Hartman GD Coleman PJ 《Bioorganic & medicinal chemistry letters》2008,18(4):1425-1430
A series of OX(2)R/OX(1)R dual orexin antagonists was prepared based on a proline bis-amide identified as a screening lead. Through a combination of classical and library synthesis, potency enhancing replacements for both amide portions were discovered. N-methylation of the benzimidazole moiety within the lead structure significantly reduced P-gp susceptibility while increasing potency, giving rise to good brain penetration. A compound from this series has demonstrated in vivo central activity when dosed peripherally in a pharmacodynamic model of orexin activity. 相似文献
27.
Where tributaries meet, certain conditions of flow and topography often result in incomplete mixing and the formation of spatially
and temporally persistent plumes or bands. Yolo Bypass, the primary floodplain of the lower Sacramento River (California,
USA), provides an extreme example of this effect. Inspection of recent and historical aerial photographs revealed that the
four major tributaries of Yolo Bypass typically do not substantially mix laterally within the floodplain. The phenomenon is
notable in the number of tributaries involved (4), the distance over which the bands remain distinct (>61 km), and the persistence
of the bands despite channel constrictions and long cross-wind fetch. This effect demonstrates the importance of lateral variability
during floodplain flow events, including transport and distribution of chemical constituents, and habitat for fish and other
organisms that use floodplains as migration corridors and rearing areas.
Handling editor: S. M. Thomaz 相似文献
28.
Meloxicam Blocks Neuroinflammation,but Not Depressive-Like Behaviors,in HIV-1 Transgenic Female Rats
Christina L. Nemeth Erica R. Glasper Constance S. Harrell Sanjana A. Malviya Jeffrey S. Otis Gretchen N. Neigh 《PloS one》2014,9(10)
Adolescents living with human immunodeficiency virus (HIV) comprise approximately 12% of the HIV-positive population worldwide. HIV-positive adolescents experience a higher rate of clinical depression, a greater risk of sexual and drug abuse behaviors, and a decreased adherence to highly active antiretroviral therapies (HAART). Using adolescent HIV-1 transgenic rats (HIV-1 tg) that display related immune response alterations and pathologies, this study tested the hypothesis that developmental expression of HIV-1-related proteins induces a depressive-like phenotype that parallels a decrease in hippocampal cell proliferation and an increase in pro-inflammatory cytokine expression in the hippocampus. Consistent with this hypothesis, adolescent HIV-1 tg rats demonstrated a depressive-like behavioral phenotype, had decreased levels of cell proliferation, and exhibited elevated expression of monocyte chemotactic protein-1 (Mcp-1) in the hippocampus relative to controls. Subsequently, we tested the ability of meloxicam, a selective COX-2 inhibitor, to attenuate behavioral deficits via inflammatory mechanisms. Daily meloxicam treatments did not alter the behavioral profile despite effectively reducing hippocampal inflammatory gene expression. Together, these data support a biological basis for the co-morbid manifestation of depression in HIV-positive patients as early as in adolescence and suggest that modifications in behavior manifest independent of inflammatory activity in the hippocampus. 相似文献
29.
Pathways of glycosphingolipid biosynthesis in SW13 cells in the presence and absence of vimentin intermediate filaments 总被引:2,自引:2,他引:0
We reported previously that the incorporation of sugars intoglycosphingolipids (GSL) is diminished in SW13 cells that lacka vimentin intermediate filament (IF) network (vim) comparedto vim+ cells. To further analyze the nature of this abnormality,we double-labeled cells with 3H-serine and l4C-sugars. Therewas no difference between vim+ and vim cells in the incorporationof serine into GSL, although the usual difference in sugar incorporationwas observed. This indicated that the defect in vim cellswas not in the incorporation of sugars into ceramide synthesizedde novo by acylation of sphinganine (pathway 1). Sugars canalso be incorporated into ceramide synthesized from sphingosinethat is derived from catabolism of sphin-golipids (pathway 2),and into GSL that recycle through the Golgi apparatus from endosomes(pathway 3). The amount of galactose and glucosamine incorporatedinto GSL in these three pathways was analyzed by the use oftwo inhibitors of sphingolipid biosynthesis. ß-Chloroala-nineinhibits the de novo synthesis of sphinganine (pathway 1), andfumonisin Bl inhibits the acylation of sphinganine and sphingosine(pathways 1 and 2). We were surprised to observe that in bothvim+ and vim cells only 2040% of sugar incorporationinto GSL took place in pathway 1, and 6080% of sugarincorporation took place in the recycling pathways. Moreover,in contrast to larger GSL, GlcCer was not synthesized in pathway3. Our observations indicate that vimentin IF facilitate therecycling of GSL and sphingosine, and that the differences betweenvim+ and vim cells are predominantly in pathways 2 and3. Furthermore, although it is generally believed that virtuallyall GSL are synthesized in the de novo pathway, these data indicatethat the recyling pathways predominate in the incorporationof sugars into GSL in SW13 cells. glycosphingolipid biosynthesis intermediate filaments SW13 cells sphingolipid recycling vimentin 相似文献
30.
Use of Acetone-Dried Vaccines for Preparing Capsular Antisera Against the Klebsiella Group and the Lyophilization of Klebsiella Cultures
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James H. Green Nan E. Pigott Richard C. Bolin William K. Harrell 《Applied microbiology》1970,20(3):416-420
Capsular antisera against the 72 types of Klebsiella have been prepared in rabbits by using an acetone-dried vaccine. It was shown that encapsulated cells dried with acetone and ground to a fine powder with a mortar and pestle retain their capsules. Antisera obtained from rabbits vaccinated with these vaccines had quellung titers ranging from 1:16 to 1:64. The dried vaccine was stable after storage for over 2 years at room temperature. Encapsulated cultures lyophilized in the presence of 5% sucrose, 5% sodium glutamate, and 5% polyvinylpyrrolidone remained viable and retained their capsules after storage for 10 months at room temperature. 相似文献