Cyclic AMP formation of isolated human corpora lutea in response to HCG - interference by PGF2 alpha.

Human corpora lutea of defined ages were excised at operation, cut into pieces and incubated in the presence of HCG, PGF2 alpha and PGE2 alone or in combination. Following incubation cAMP formation in tissue and medium was determined. HCG-stimulated tissue cAMP content was most pronounced at a corpus luteum age of 7-10 days after ovulation. This stimulation was antagonized by PGF2 alpha in corpora lutea older than 6 days. PGE2 stimulated cAMP formation per se and this effect was more pronounced when HCG and PGE2 were combined. A possible role for PGF2 alpha as a luteolytic substance in the human is suggested.     

Conformation of methylated sequences in HeLa cell 18-S ribosomal RNA: nuclease S1 as a probe

18-S rRNA from HeLa cells was digested with nuclease S1. Under the conditions employed 15% of the total nucleotides and some 50% of the methylated nucleotides were released as low-molecular-weight products. The material which was precipitable by 70% ethanol after nuclease S1 digestion was subjected to further digestion by combined T1 plus pancreatic ribonucleases or by T1 ribonuclease alone, and fingerprints were prepared. It was found that the four sites which are modified late during ribosome maturation, and which contain base modifications, were all accessible to nuclease S1. By contrast fewer than one-half of the sites which are modified early during ribosome maturation, and which contain 2'-O-methyl groups, were accessible to nuclease S1; the remainder were protected, presumably by secondary or tertiary interactions within 18-S rRNA.     

Heterogeneous staining of rat hepatocyte nuclei by HBFP technique

Synopsis The non-enzymatic histochemical technique Haematoxylin-Basic Fuchsinpicric acid (HBFP) was studied in fresh-frozen and Carnoy-fixed, paraffin-embedded rat liver sections. The hepatocyte nuclei fell into two populations and showed either a crimson red or purple staining in frozen as well as paraffin sections. The heterogeneous staining of the rat heptocyte nuclei was also present when the tissue sections were stained by Methyl Green-Pyronin stain. The differing nuclear staining was present in the isolated nuclei also. The HBFP technique, therefore, appears potentially useful when applied to liver and other tissues as well.     

Preliminary observations on plant-rock type relationships in the Shiraz-Dashte Arjan area,South-West Iran

Summary In the Dashte-Arjan area near Shiraz, rock formations appear to control the distribution of various plant species. The Fars formation (Miocene) and the Asmari-Jahrom formation (Eocene-Oligocen) sediments are characterized by distinet plant species and life forms. Among the characteristic calciphytes of Asmari-Jahrom limestones are Astragalus acutus, Amygdalus lyciodes, Cerasus microcarpa, and Fraxinus rotundifolia. The Fars formation limestones are characterized by Astragulus gossypinus, Acantholimon flexuosum, Noaea mucronata and Phlomis bruguieri, Except for Glycrrhiza glabra, white and red marls seem to have similar species, such as Alhagi maurorum and Carthamus oxyacantha. Gypsum of Fars formation has Berberis integerrima and Rosa beggeriana. Various life forms have been distinguished and were found to be confined to different rock types as well.Nomenclature of species is given in table 1.We are greately indebted to Dr. P.H. Davis and Prof. G. Pontecorvo F.R.S. for critically reading this paper and making some invaluable suggestions. We are grateful to the University Research Grant Commission for providing us a grant for this work. We are also indebted to the Dean, College of Arts & Sciences, Pahlavi University, Shiraz, Iran for providing us with a vehicle during this work.     

Successful phytoremediation of crude-oil contaminated soil at an oil exploration and production company by plants-bacterial synergism

Phytoremediation is a promising approach for the cleanup of soil contaminated with petroleum hydrocarbons. This study aimed to develop plant-bacterial synergism for the successful remediation of crude oil-contaminated soil. A consortia of three endophytic bacteria was augmented to two grasses, Leptochloa fusca and Brachiaria mutica, grown in oil-contaminated soil (46.8 g oil kg^?1 soil) in the vicinity of an oil exploration and production company. Endophytes augmentation improved plant growth, crude oil degradation, and soil health. Maximum oil degradation (80%) was achieved with B. mutica plants augmented with the endophytes and it was significantly (P < 0.05) higher than the use of plants or bacteria individually. Moreover, endophytes showed more persistence, the abundance and expression of alkB gene in the rhizosphere as well as in the endosphere of the tested plants than in unvegetated soil. A positive relationship (r = 0.70) observed between gene expression and crude oil reduction indicates that catabolic gene expression is important for hydrocarbon mineralization. This investigation showed that the use of endophytes with appropriate plant is an effective strategy for the cleanup of oil-contaminated soil under field conditions.     

Exclusive transmission of the embryonic stem cell‐derived genome through the mouse germline

Gene targeting in embryonic stem (ES) cells remains best practice for introducing complex mutations into the mouse germline. One aspect in this multistep process that has not been streamlined with regard to the logistics and ethics of mouse breeding is the efficiency of germline transmission: the transmission of the ES cell‐derived genome through the germline of chimeras to their offspring. A method whereby male chimeras transmit exclusively the genome of the injected ES cells to their offspring has been developed. The new technology, referred to as goGermline, entails injecting ES cells into blastocysts produced by superovulated homozygous Tsc22d3 floxed females mated with homozygous ROSA26‐Cre males. This cross produces males that are sterile due to a complete cell‐autonomous defect in spermatogenesis. The resulting male chimeras can be sterile but when fertile, they transmit the ES cell‐derived genome to 100% of their offspring. The method was validated extensively and in two laboratories for gene‐targeted ES clones that were derived from the commonly used parental ES cell lines Bruce4, E14, and JM8A3. The complete elimination of the collateral birth of undesired, non‐ES cell‐derived offspring in goGermline technology fulfills the reduction imperative of the 3R principle of humane experimental technique with animals. genesis 54:326–333, 2016. © 2016 The Authors. Genesis Published by Wiley Periodicals, Inc.     

Dairy manure and tillage effects on soil fertility and corn yields

Organic amendments have received renewed attention to improve soil fertility for crop production. A randomized complete block split plot experiment was conducted to evaluate the dairy manure (DM) amendments of soil for corn (Zea mays L. cv. Monsanto 919) production under different tillage systems. Main plot treatments were no-till (NT), conventional tillage (CT), and deep tillage (DT), and subplot treatments were chemical fertilization (DM(0)), and DM at 10Mgha(-1)yr(-1) (DM(10)) and 20Mgha(-1)yr(-1) (DM(20)) with supplemental chemical fertilization. Results show that tillage and DM had significantly reduced bulk density (rho(b)) with greater porosity (f(t)) and hydraulic conductivity (K(fs)) than soils under NT and DM(0). Manuring was effective to improve soil physical properties in all tillage treatments. While manure significantly increased C sequestration, the N concentration was influenced by both tillage and manure with significant interaction. The CT significantly increased P as did the addition of manure. However, with manure, K was significantly increased in all tillage treatments. While tilled soils produced taller plants with higher grain yields, and water-use efficiency than NT soils, manuring, in contrast, increased corn harvest index. Manure exerted significant quadratic effect on corn biomass N and K uptake. The variable effects of tillage and dairy manuring on soil properties and corn growth are most probably related to "transitional period" in which soil ecosystems may have adjusting to a new equilibrium.     

Mapping the interactions between a RUN domain from DENND5/Rab6IP1 and sorting nexin 1

Eukaryotic cells have developed a diverse repertoire of Rab GTPases to regulate vesicle trafficking pathways. Together with their effector proteins, Rabs mediate various aspects of vesicle formation, tethering, docking and fusion, but details of the biological roles elicited by effectors are largely unknown. Human Rab6 is involved in the trafficking of vesicles at the level of Golgi via interactions with numerous effector proteins. We have previously determined the crystal structure of Rab6 in complex with DENND5, alternatively called Rab6IP1, which comprises two RUN domains (RUN1 and RUN2) separated by a PLAT domain. The structure of Rab6/RUN1-PLAT (Rab6/R1P) revealed the molecular basis for Golgi recruitment of DENND5 via the RUN1 domain, but the functional role of the RUN2 domain has not been well characterized. Here we show that a soluble DENND5 construct encompassing the RUN2 domain binds to the N-terminal region of sorting nexin 1 by surface plasmon resonance analyses.