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11.
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C B Harley J Lawrie M Betlach R Crea H W Boyer J Hedgpeth 《Nucleic acids research》1988,16(15):7269-7285
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Reversible inhibition of intercellular junctional communication by glycyrrhetinic acid 总被引:17,自引:0,他引:17
J S Davidson I M Baumgarten E H Harley 《Biochemical and biophysical research communications》1986,134(1):29-36
Intercellular gap-junctional communication was measured using metabolic co-operation in co-cultures of argininosuccinate synthetase-deficient and argininosuccinate lyase-deficient human fibroblasts. 18-alpha-glycyrrhetinic acid (AGA) was found to inhibit communication by more than 95% at concentrations as low as 2 microM. Concentrations up to 100 microM were not cytotoxic over a period of 2 hours. Communication inhibition was of rapid onset and was readily reversible. Communication remained continuously yet reversibly blocked in cells cultured in the presence of AGA for 20 days. The related compounds 18-beta-glycyrrhetinic acid and carbenoxolone also caused communication inhibition. The effect is probably not mediated via mineralocorticoid or glucocorticoid receptors since aldosterone and glucocorticoids had no effect on communication. AGA thus has properties of a useful inhibitor in the study of intercellular junctional communication. 相似文献
15.
Regional localisations and linkage relationships of seven RFLPs and myotonic dystrophy on chromosome 19 总被引:4,自引:2,他引:2
D. J. Shaw A. L. Meredith M. Sarfarazi H. G. Harley S. M. Huson J. D. Brook L. Bufton M. Litt T. Mohandas P. S. Harper 《Human genetics》1986,74(3):262-266
Summary We have studied the genetic linkage relationships of seven DNA polymorphisms on chromosome 19, with each other and with the myotonic dystrophy locus. The DNA sequences were localised to various regions of the chromosome using translocations in somatic cell hybrids. These results provide the basis for a linkage map of most of chromosome 19, and suggest that the myotonic dystrophy locus is close to the centromere. 相似文献
16.
Determination of Rubella Hemagglutination-Inhibiting Antibody in Whole Blood 总被引:1,自引:0,他引:1 下载免费PDF全文
A technique is described for determination of rubella hemagglutination-inhibiting antibody in only 50 to 100 muliters of whole blood. 相似文献
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Brian J. S. Harley 《CMAJ》1960,82(22):1123-1126
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Lysis of sensitized sheep erythrocytes in human sera deficient in the second component of complement 总被引:1,自引:0,他引:1
K L Knutzen Steuer L B Sloan T J Oglesby T C Farries M W Nickells P Densen J B Harley J P Atkinson 《Journal of immunology (Baltimore, Md. : 1950)》1989,143(7):2256-2261
Analysis of C-dependent lysis of sensitized SRBC by C2-deficient sera (C2D) led to the characterization of a C2 bypass pathway. Lysis in the total hemolytic C assay by C2D sera was Ca2+-dependent and required a high concentration of hemolysin to sensitize E. Selective component depletion indicated a requirement for C1 and C4 of the classical pathway (CP) and proteins B, P, and probably D of the alternative pathway (AP). Total hemolytic C could be restored to normal in these C2D sera by utilizing heavily sensitized E or by the addition of a supranormal concentration of B. This system most closely resembles a pathway described by J. E. May and M. M. Frank which requires antibody, C1, and the AP but not C4 or C2. It differs in its requirement for C4. We hypothesize that this pathway represents vestiges of a more primitive C pathway. It becomes evident and possibly clinically important in the setting of C2 deficiency, by allowing C activation, other than the AP, and perhaps in normal individuals, by damaging microorganisms that have evolved means to inhibit early components of the CP. 相似文献