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排序方式: 共有419条查询结果,搜索用时 46 毫秒
411.
P. Ramu B. Kassahun S. Senthilvel C. Ashok Kumar B. Jayashree R. T. Folkertsma L. Ananda Reddy M. S. Kuruvinashetti B. I. G. Haussmann C. T. Hash 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(7):1193-1204
The sequencing and detailed comparative functional analysis of genomes of a number of select botanical models open new doors
into comparative genomics among the angiosperms, with potential benefits for improvement of many orphan crops that feed large
populations. In this study, a set of simple sequence repeat (SSR) markers was developed by mining the expressed sequence tag
(EST) database of sorghum. Among the SSR-containing sequences, only those sharing considerable homology with rice genomic
sequences across the lengths of the 12 rice chromosomes were selected. Thus, 600 SSR-containing sorghum EST sequences (50
homologous sequences on each of the 12 rice chromosomes) were selected, with the intention of providing coverage for corresponding
homologous regions of the sorghum genome. Primer pairs were designed and polymorphism detection ability was assessed using
parental pairs of two existing sorghum mapping populations. About 28% of these new markers detected polymorphism in this 4-entry
panel. A subset of 55 polymorphic EST-derived SSR markers were mapped onto the existing skeleton map of a recombinant inbred
population derived from cross N13 × E 36-1, which is segregating for Striga resistance and the stay-green component of terminal drought tolerance. These new EST-derived SSR markers mapped across all
10 sorghum linkage groups, mostly to regions expected based on prior knowledge of rice–sorghum synteny. The ESTs from which
these markers were derived were then mapped in silico onto the aligned sorghum genome sequence, and 88% of the best hits corresponded to linkage-based positions. This study demonstrates
the utility of comparative genomic information in targeted development of markers to fill gaps in linkage maps of related
crop species for which sufficient genomic tools are not available. 相似文献
412.
413.
Chandan Prasad A. Jayaraman Hugh J. F. Robertson Jayashree K. Rao 《Neurochemical research》1987,12(9):767-774
Cyclo(His-Pro), or histidyl-proline diketopiperazine, is an endogenous cyclic dipeptide that is ubiquitously distributed in tissues and body fluids of both man and animals. This cyclic dipeptide is not only structurally related to thyrotropin-releasing hormone (TRH, pGlu-His-ProNH2), but it can also arise from TRH by the action of the enzyme pyroglutamate amino-peptidase (pGlu-peptidase). The data on the distribution of TRH, cyclo(His-Pro), and pGlu-peptidase under normal and abnormal conditions are summarized and potential relationships analyzed. We conclude that all of the cyclo(His-Pro) cannot be derived from TRH. Two additional sources of cyclo(His-Pro) are suggested. It is proposed that 29,247 molecular weight TRH prohormone, prepro TRH, which contains 5 copies of TRH sequence, can be processed to yield cyclo(His-Pro). Thus, both TRH and cyclo(His-Pro) share a common precursor, prepro[TRH/Cyclo(His-Pro)]. 相似文献
414.
Summary A spectrophotometric assay for cephalosporin C was established usingAlcaligenes faecalis as a test organism. The optimal pH range for the sensitivity of the assay was pH 6.7–7.4. The dose-response curve became linear after 4 h incubation with 15% (v/v) dosage. Deviations between spectrophotometric and chemical assays were within 6%. The cost of the spectrophotometric assay is about one-hundredth that of the chemical assay. 相似文献
415.
Selvan Nehru John Abraham Anitha Priya Sekar Hariharan Rajadurai Vijay Solomon Selvakumar Veeralakshmi 《Journal of biomolecular structure & dynamics》2020,38(7):2057-2067
AbstractFor efficient designing of metallodrugs, it is imperative to analyse the binding affinity of those drugs with drug-carrying serum albumins to comprehend their structure–activity correlation for biomedical applications. Here, cobalt(II) and cobalt(III) complexes comprising three phendione ligands, [Co(phendione)3]Cl2 (1) and [Co(phendione)3]Cl3 (2), where, phendione = 1,10-phenanthroline-5,6-dione, has been chosen to contrast the impact of their hydrophobicity and ionicity on binding with bovine serum albumin (BSA) through spectrophotometric titrations. The attained hydrophobicity values using octanol/water partition coefficient method manifested that complex 1 is more hydrophobic than complex 2, which could be attributed to lesser charge on its coordination sphere. The interaction of complexes 1 and 2 with BSA using steady state fluorescence studies revealed that these complexes quench the intrinsic fluorescence of BSA through static mechanism, and the extent of quenching and binding parameters are higher for complex 2. Further thermodynamics of BSA-binding studies revealed that complexes 1 and 2 interact with BSA through hydrophobic and hydrogen bonding/van der Waals interactions, respectively. Further, UV–visible absorption, circular dichroism and synchronous fluorescence studies confirmed the occurrence of conformational and microenvironmental changes in BSA upon binding with complexes 1 and 2. Molecular docking studies have also shown that complex 2 has a higher binding affinity towards BSA as compared to complex 1. This sort of modification of ionicity and hydrophobicity of metal complexes for getting desirable binding mode/strength with drug transporting serum albumins will be a promising pathway for designing active and new kind of metallodrugs for various biomedical applications.Communicated by Ramaswamy H. Sarma 相似文献
416.
417.
Priya Hariharan Madhavi Sawant Manju Gorivale Ruma Manchanda Roshan Colah K. Ghosh Anita Nadkarni 《Molecular biology reports》2017,44(5):413-417
Co-inheritance of gamma and beta globin gene mutations in a compound heterozygous state is rare but of clinical interest as it provides an important data on understanding the HbF expression. Hematological analysis was carried out (Sysmex KX-21). F-cells were enumerated using flow cytometry. Beta globin gene was analysed by CRDB technique and by DNA sequencing. Gamma globin promoter region was sequenced and expression studies were carried out using real time Taqman assay. We report a family, where two inherited defects of the β globin gene cluster segregate. The proband and her sibling were compound heterozygotes for a novel Gγ promoter mutation and the 619 bp deletion a common Indian β thalassemia mutation. Molecular characterization revealed that the father (HbA2 5.1%, HbF 5.4%), proband (HbA2 3.6%, HbF 31.7%) and her brother (HbA2 3.9%, HbF 23.6%) were heterozygous for the 619 bp deletion. The mother (HbA2 2.1%, HbF 3.4%) had a normal β globin gene. As both the children showed high HbF levels, the γ globin gene work up was carried out. The Gγ-globin gene promoter analysis revealed that the mother and the two children were heterozygous for a 5 bp deletion -ATAAG (-533 to -529) that resides in the GATA binding site. These findings suggest that the 5 bp deletion in the Gγ globin promoter has a functional role in silencing the γ-globin gene expression in adults by disrupting GATA-1 binding and the associated repressor complex and results in the up-regulation of gamma globin gene expression. When co-inherited with β -thalassemia trait it leads to a phenotype of HPFH. 相似文献
418.
Functional & Integrative Genomics - Campylobacter jejuni remains a major cause of human gastroenteritis with estimated annual incidence rate of 450 million infections worldwide. C. jejuni is a... 相似文献
419.
Plasmonics - This study presents an in situ growth technique to develop highly sensitive plasmonic fiber optic sensors with an excellent control over the plasmonic properties of gold (AuNPs) and... 相似文献