全文获取类型
收费全文 | 148篇 |
免费 | 7篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 7篇 |
2020年 | 5篇 |
2019年 | 2篇 |
2018年 | 1篇 |
2017年 | 4篇 |
2016年 | 9篇 |
2015年 | 9篇 |
2014年 | 6篇 |
2013年 | 14篇 |
2012年 | 14篇 |
2011年 | 10篇 |
2010年 | 7篇 |
2009年 | 3篇 |
2008年 | 6篇 |
2007年 | 7篇 |
2006年 | 6篇 |
2005年 | 1篇 |
2004年 | 4篇 |
2003年 | 2篇 |
2002年 | 2篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1999年 | 2篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1991年 | 5篇 |
1990年 | 2篇 |
1988年 | 4篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1977年 | 2篇 |
1975年 | 1篇 |
1972年 | 2篇 |
排序方式: 共有155条查询结果,搜索用时 15 毫秒
71.
B-Rao C Kulkarni-Almeida A Katkar KV Khanna S Ghosh U Keche A Shah P Srivastava A Korde V Nemmani KV Deshmukh NJ Dixit A Brahma MK Bahirat U Doshi L Sharma R Sivaramakrishnan H 《Bioorganic & medicinal chemistry》2012,20(9):2930-2939
In recent years, xanthine oxidase has emerged as an important target not only for gout but also for cardiovascular and metabolic disorders involving hyperuricemia. Contrary to popular belief, recent clinical trials with uricosurics have demonstrated that enhanced excretion of uric acid is, by itself, not adequate to treat hyperuricemia; simultaneous inhibition of production of uric acid by inhibition of xanthine oxidase is also important. Virtual screening of in-house synthetic library followed by in vitro and in vivo testing led to the identification of a novel scaffold for xanthine oxidase inhibition. In vitro activity results corroborated the results from molecular docking studies of the virtual screening hits. The isocytosine scaffold maintains key hydrogen bonding and pi-stacking interactions in the deep end of the xanthine-binding pocket, which anchors it in an appropriate pose to inhibit binding of xanthine and shows promise for further lead optimization using structure-based drug design approach. 相似文献
72.
Shahana Perveen Hardik Patel Arslan Arif Sharif Younis Champa N. Codipilly Mohamed Ahmed 《PloS one》2012,7(12)
Angiogenesis is one of the most important processes for normal lung development. Oxidative stress can impair the pulmonary angiogenesis, leading to chronic lung disease or Bronchopulmonary dysplasia (BPD).
Objective
To investigate the protective effects of EC-SOD overexpression on pulmonary angiogenesis on neonates following exposure to acute hyperoxia.Design/Methods
Transgenic (TG) and wild-type (WT) neonatal mice (10 mice per group) were exposed either to air (control group) or 95% O2 for 7 days starting at birth. After exposure, all animals were sacrificed. ROS concentration was measured in lung homogenates using OxiSelect ROS assay kit. Mean vascular density (MVD) was measured using anti CD34 staining. RNA was extracted and the angiogenesis markers, VEGF, VEGFR1 and VEGFR2 and PECAM-1 were analyzed by RT-q PCR. VGEF protein was measured using Western blots. Endothelial progenitor cells (EPCs) was assayed by flow cytometer.Results
There was a significant reduction of ROS in TG hyperoxic neonate group (156±14.2) compared to WT hyperoxic animals (255±35.1). Evaluation of MVD, using anti-CD34, showed marked significant increase of MVD in the TG group following hyperoxic exposure (85±12) in comparison to the WT hyperoxic group (62±8.4), (P<0.05). Among the hyperoxic groups, both RNA and protein of VEGF expression were significantly reduced in the WT animals compared to the TG group (P<0.05). The same trend was found in VEGFR 1 and 2 which were significantly reduced in WT group compared to the TG group (P<0.05). There was no significant difference between hyperoxia TG and control group (P>0.05). PECAM expression was significantly reduced in both hyperoxic compared to normoxic groups (P<0.05). EPC’s showed significant reduction in WT hyperoxic group compared to others (P>0.05).Conclusions
EC-SOD plays a key role in preserving angiogenesis by scavenging free radicals which has an inhibitory effect on angiogenesis process in neonatal mice lung following exposure to hyperoxia. 相似文献73.
74.
75.
A method is described for the simultaneous analysis of several biologically important phenylethylamines using gas chromatography/mass spectrometry. The amines are converted to N-dinitrophenyl, O-trimethylsilyl derivatives prior to their separation by gas chromatography. By using selected ion monitoring in the chemical ionization mode, we have been able to quantitate the endogenous concentrations of phenylethylamine and phenylethanolamine in rat brain. 相似文献
76.
Olgun N Patel HJ Stephani R Wang W Yen H Reznik SE 《Canadian journal of physiology and pharmacology》2008,86(8):571-575
Preterm birth (PTB), defined as any birth occurring before 37 weeks of gestation, occurs in only 12% of all births, yet accounts for nearly half of long-term neurological morbidity, and 60%-80% of perinatal mortality. The single most common cause of PTB is intrauterine infection. Endothelin-1 (ET-1) is a potent vasoconstrictor peptide that is both upregulated by inflammatory cytokines and capable of increasing myometrial smooth muscle tone. We hypothesized, therefore, that ET-1 is a critical component of the parturition cascade in the setting of infection-associated PTB. In our previous work, we have shown that blockade of ET-1 synthesis through the use of the metalloproteinase inhibitor phosphoramidon results in control of preterm labor. In the current work, we showed that blockade of ET-1 action with 5-50 mg/kg i.p. 3-(3-carboxybenzyl)-1-((6-ethylbenzo[d][1,3]dioxol-5-yl)methyl)-6-hydroxy-4-oxo-1,4-dihydroquinoline-2-carboxylic acid (HJP272), a putative novel selective ETA-receptor antagonist (IC50, 70 nmol/L), prevents PTB induced with up to 50 mg/kg of i.p. lipopolysaccharide in a mouse model. This is the first report, to our knowledge, of control of infection-associated PTB with a specific ETA-receptor antagonist. The identification of a novel effective therapy for PTB could have important clinical implications. 相似文献
77.
The biosorption of metal ions (Cr+3,
, Cu+2, and Ni+2) on two algal blooms (designated HD-103 and HD-104) collected locally was investigated as a function of the initial metal
ion concentration. The main constituent of HD-103 is Cladophora sp., while Spirulina sp. is present significantly in the bloom HD-104. Algal biomass HD-103 exhibited the highest Cu+2 uptake capacity (819 mg/g). This bloom adsorbed Ni+2 (504 mg/g), Cr+3 (347 mg/g), and (168 mg/g). Maximum of Ni+2 (1108 mg/g) is taken by HD-104. This species takes up 306, 202, and 576 mg/g Cr+3, , and Cu+2, respectively. Equilibrium data fit very well to both the Langmuir and the Freundlich isotherm models. The sorption process
followed the Freundlich model better. Pseudo-first-order kinetic model could describe the kinetic data. Infrared (IR) spectroscopic
data were employed to identify the site(s) of bonding. It was found that phosphate and peptide moieties participate in the
metal uptake by bloom HD-103. In the case of bloom HD-104, carboxylate and phosphate are responsible for the metal uptake.
The role of protein in metal uptake by HD-103 was investigated using polyacrylamide gel electrophoresis. 相似文献
78.
Efficient nonviral transfection of dendritic cells and their use for in vivo immunization 总被引:4,自引:0,他引:4
Irvine AS Trinder PK Laughton DL Ketteringham H McDermott RH Reid SC Haines AM Amir A Husain R Doshi R Young LS Mountain A 《Nature biotechnology》2000,18(12):1273-1278
Immunization with dendritic cells (DCs) transfected with genes encoding tumor-associated antigens (TAAs) is a highly promising approach to cancer immunotherapy. We have developed a system, using complexes of plasmid DNA expression constructs with the cationic peptide CL22, that transfects human monocyte-derived DCs much more efficiently than alternative nonviral agents. After CL22 transfection, DCs expressing antigens stimulated autologous T cells in vitro and elicited primary immune responses in syngeneic mice, in an antigen-specific manner. Injection of CL22-transfected DCs expressing a TAA, but not DCs pulsed with a TAA-derived peptide, protected mice from lethal challenge with tumor cells in an aggressive model of melanoma. The CL22 system is a fast and efficient alternative to viral vectors for engineering DCs for use in immunotherapy and research. 相似文献
79.
Kumar PS Soni K Jadhav SR Doshi NS Saraf MN 《Indian journal of experimental biology》2007,45(5):419-424
The effect of chloroform soluble fraction (F-A) of twigs of Sarcostemma brevistigma on contractions induced by KCl, histamine, and acetylcholine in the isolated guinea pig ileum and taenia coli smooth muscles has been evaluated. F-A (19.5 microg/ml) significantly inhibited the contraction induced by 40 mM KCl to the extent of 87.6% in the isolated guinea pig ileum. In the isolated guinea pig ileum, F-A (64.3 and 59.2 microg/ml) significantly inhibited the contractions induced by acetylcholine and histamine to the extent of 85 and 83% respectively. In the isolated guinea pig taenia coli, F-A (65.2 microg/ml) significantly inhibited the contraction induced by 40 mM KCl to the extent of 96.0%. The inhibitory effect of F-A (40 microg/ml) on the isolated guinea pig taenia coli was reduced by Bay K 8644 (10(-6) M) to the extent of 61.6 from 73.6%. These results suggest that the F-A may exhibit smooth muscle relaxant activity by blocking the Ca2+ channels. 相似文献
80.
Metal binding by algae has enormous potential for environmental bioremediation targeting towards detoxification of water bodies. The present work reports the use of live and dead Spirulina sp. for sorption of metals like Cr(3+), Ni(2+), Cu(2+), and Cr(6+) in form of Cr(2)O(7)(2-). Preliminary investigation shows that this biomass takes up substantial amount of metal ions indicated above. IR spectroscopic study, kinetics models, Langmuir and Freundlich adsorption isotherms, SEM analysis, and fluorescence microscopic study of Spirulina sp. and the Spirulina sp. treated with different metal ions have been employed to understand the sorption mechanism. It is hoped that live Spirulina sp. will be a strong candidate for management of industrial wastewater. 相似文献