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991.
Yang Xiaolin Sun Beibei Gao Wangsheng Chen Yuanquan Sui Peng 《The International Journal of Life Cycle Assessment》2019,24(3):371-385
The International Journal of Life Cycle Assessment - Low carbon footprint agriculture has received increasing attention in the effect of reducing greenhouse gas emissions and mitigating climate... 相似文献
992.
Mammalian Genome - Increasing evidence shows that miRNAs play pivotal roles in cardiovascular diseases, including heart failure (HF). The aim of this study was to investigate the role of miR-129-5p... 相似文献
993.
994.
995.
G L Chen A Balfe W Erwa G Hoefler J Gaertner J Aikawa W W Chen 《Biochemical and biophysical research communications》1991,178(3):1084-1091
A polypeptide containing the carboxyl-terminal fragment of human peroxisomal enoyl-CoA hydratase:3-hydroxyacyl-CoA dehydrogenase bifunctional enzyme was synthesized in vitro from its cDNA clone. This expression polypeptide was transported into purified rat liver peroxisomes. When the expression polypeptide was incubated with postnuclear supernatant fractions of human hepatoma cells and analyzed by Nycodenz gradient SDS-PAGE and fluorography, it was imported specifically into peroxisomes as indicated by its resistance to proteinase K degradation. A deletion of the last nine amino acid residues at the carboxyl-terminus of this polypeptide prevents its peroxisomal import. A tripeptide sequence, SKL, located at the carboxyl-terminus of human bifunctional enzyme appears to be the targeting signal for the peroxisomal importation of bifunctional enzyme in human cells. 相似文献
996.
Bioprocess and Biosystems Engineering - The properties of the anode material and structure are critical to the microbial growth and interfacial electron transfer between the biofilm and the anode.... 相似文献
997.
998.
Zhaodong Huang Ao Chen Funian Mo Guojin Liang Xinliang Li Qi Yang Ying Guo Ze Chen Qing Li Binbin Dong Chunyi Zhi 《Liver Transplantation》2020,10(24)
Output voltage and self‐discharge rate are two important performance indices for supercapacitors, which have long been overlooked, though these play a very significant role in their practical application. Here, a zinc anode is used to construct a zinc ion hybrid capacitor. Expanded operating voltage of the hybrid capacitor is obtained with novel electrolytes. In addition, significantly improved anti‐self‐discharge ability is achieved. The phosphorene‐based zinc ion capacitor exploiting a “water in salt” electrolyte with a working potential can reach 2.2 V, delivering 214.3 F g?1 after 5000 cycles. The operating voltage is further extended to 2.5 V through the use of an organic solvent as the electrolyte; the solvent is prepared by adding 0.2 m ZnCl2 into the tetraethylammonium tetrafluoroborate in propylene carbonate (Et4NBF4/PC) solvent, and it exhibits 105.9 F g?1 even after 9500 cycles. More importantly, the phosphorene‐based capacitors possess excellent anti‐self‐discharge performance. The capacitors retain 76.16% of capacitance after resting for 300 h. The practical application of the zinc ion capacitor is demonstrated through a flexible paper‐based printed microcapacitor. It is believed that the developed zinc ion capacitor can effectively resolve the severe self‐discharge problem of supercapacitors. Moreover, high‐voltage zinc ion capacitors provide more opportunities for the application of supercapacitors. 相似文献
999.
Washing is a standard step for enzyme‐linked immunosorbent assays (ELISA) performed on a paper‐based chip, in which nonspecific‐binding antibodies and antigens should be removed completely from the paper surface. In this study, a novel three‐dimensional (3D) washing strategy using a heating ring‐oven was carried out on a paper‐based chip. Compared with a plane washing mode by a ring‐oven, this 3D washing strategy obtained a lower background, as gravity played an important role in the washing step. The paper‐based chip was placed on a 3D plastic holder and the waste area was connected to a heating ring. Use of a heating waste area meant that the nonspecific‐binding protein was continuously carried to the waste area through gravity and capillary action. The angle between the plastic holder and the ring plane was carefully selected. The effect of washing on different parts of the detection area was investigated by upconversion fluorescence and chemiluminescence (CL). This novel 3D washing strategy was performed for carcinoembryonic antigen detection through CL and a lower detection limit of 2 pg ml?1 was obtained. This approach provides an effective washing strategy to remove nonspecific‐binding antibody from a paper‐based immunodevice. 相似文献
1000.
Chen X Shang H Qiu X Fujiwara N Cui L Li XM Gao TM Kong J 《Neurochemical research》2012,37(4):835-845
Converging evidence indicates that SOD1 aggregation is a common feature of mutant SOD1-linked fALS, and seems to be directly
related to the gain-of-function toxic property. However, the mechanism inducing the aggregation is not understood. To study
the contribution of oxidative modification of cysteine residues in SOD1 aggregation, we systematically examined the redox
state of SOD1 cysteine residues in the G37R transgenic mouse model at different stages of the disease and under oxidative
stress induced by H2O2. Our data suggest that under normal circumstance, cysteine 111 residue in SOD1 is free; however, under oxidative stress,
it is prone to oxidative modification by providing the thiolate anion (S−). With the progression of the disease, increased
levels of oxidative insults facilitated the oxidation of thiol groups of cysteine residues; human mutant SOD1 could generate
an upper shift band in reducing SDS-PAGE, which turned out to be a Cys111-peroxidized SOD1 species. We also detected the formation
of SOD1 multimers at different stages of the disease, and found that accumulated oxidative stress facilitated the formation
of aggregates, which were not mediated by disulfide bond. This oxidative modification of cysteine 111 therefore promotes the
formation of disulfide bond-independent aggregation of SOD1. 相似文献