全文获取类型
收费全文 | 9754篇 |
免费 | 920篇 |
国内免费 | 1246篇 |
专业分类
11920篇 |
出版年
2024年 | 53篇 |
2023年 | 216篇 |
2022年 | 461篇 |
2021年 | 698篇 |
2020年 | 521篇 |
2019年 | 556篇 |
2018年 | 516篇 |
2017年 | 393篇 |
2016年 | 533篇 |
2015年 | 676篇 |
2014年 | 867篇 |
2013年 | 833篇 |
2012年 | 994篇 |
2011年 | 851篇 |
2010年 | 471篇 |
2009年 | 472篇 |
2008年 | 496篇 |
2007年 | 438篇 |
2006年 | 322篇 |
2005年 | 277篇 |
2004年 | 245篇 |
2003年 | 157篇 |
2002年 | 174篇 |
2001年 | 80篇 |
2000年 | 85篇 |
1999年 | 66篇 |
1998年 | 53篇 |
1997年 | 47篇 |
1996年 | 34篇 |
1995年 | 41篇 |
1994年 | 37篇 |
1993年 | 23篇 |
1992年 | 29篇 |
1991年 | 31篇 |
1990年 | 31篇 |
1989年 | 33篇 |
1988年 | 14篇 |
1987年 | 19篇 |
1986年 | 11篇 |
1985年 | 9篇 |
1983年 | 11篇 |
1982年 | 8篇 |
1981年 | 4篇 |
1979年 | 3篇 |
1977年 | 6篇 |
1973年 | 3篇 |
1966年 | 2篇 |
1962年 | 2篇 |
1950年 | 2篇 |
1940年 | 2篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
81.
Leng Yu Wang Hao de Lucio Mario Gomez Hector 《Biomechanics and modeling in mechanobiology》2022,21(6):1825-1840
Biomechanics and Modeling in Mechanobiology - Subcutaneous injection of therapeutic monoclonal antibodies (mAbs) has gained increasing interest in the pharmaceutical industry. The transport,... 相似文献
82.
Xiao-Chen Chen Hao Sun Chang-Jun Zhang Ying Zhang Ke-Qin Lin Liang Yu Lei Shi Yu-Fen Tao Xiao-Qin Huang Jia-You Chu Zhao-Qing Yang 《遗传学报》2013,40(10):543-548
The ataxin-2 (ATXN2) gene is located on human chromo-some 12q24.1. In normal individuals, the coding region in exon 1 of this gene has fewer than 31 CAG repeats (Yu et al., 2005: Laffita-Mesa et al., 2012). However, an abnormal expansion of CAG trinucleotide repeats results in the aggre-gation of polyglutamine (polyQ), which causes spinocer-ebellar ataxia type 2 (SCA2) (Pulst et al., 1996). The expanded alleles have more than 32 repeats in the affected individuals, and generally there is an inverse correlation between CAG repeat length and age of onset (Pulst et al., 1996). SCA2 is an autosomal dominant inheritance neurodegenerative disease, whose major clinical feature is progressive cerebellar ataxia. Atrophies of the brainstem and frontal lobe have been frequently detected by magnetic resonance imaging (MRI) (Yamamoto-Watanabe et al., 2010). This disease has the strong effect on sensory and motor control. 相似文献
83.
Li Songtao Zhao Hongling Wang Ruxing Wang Jianping Mao Xiaoxia Hao Ting Chang Xiaomin Zhao Enhong Yin Zhifeng Deng Shuhua Yang Yaqi Wang Huina 《International journal of peptide research and therapeutics》2019,25(4):1361-1367
International Journal of Peptide Research and Therapeutics - It is demonstrated that gonadotropin-releasing hormone (GnRH) analogs can directly inhibit the proliferation of reproductive tissue... 相似文献
84.
A novel and fast-fabricated Prussian blue (PB)/topological insulator Bi(2)Se(3) hybrid film has been prepared by coelectrodeposition technique. Taking advantages of topological insulator in possessing exotic metallic surface states with bulk insulating gap, Prussian blue nanoparticles in the hybrid film have smaller size as well as more compact structure, showing excellent pH stability even in the alkalescent solution of pH 8.0. Based on the Laviron theory, the electron transfer rate constant of PB/Bi(2)Se(3) hybrid film modified electrode was calculated to be 4.05±0.49s(-1), a relatively big value which may be in favor of establishing a high-sensitive biosensor. An amperometric glucose biosensor was then fabricated by immobilizing glucose oxidase (GOD) on the hybrid film. Under the optimal conditions, a wide linear range extending over 3 orders of magnitude of glucose concentrations (1.0×10(-5)-1.1×10(-2)M) was obtained with a high sensitivity of 24.55μAmM(-1) cm(-2). The detection limit was estimated for 3.8μM defined from a signal/noise of 3. Furthermore, the resulting biosensor was applied to detect the blood sugar in human serum samples without any pretreatment, and the results were comparatively in agreement with the clinical assay. 相似文献
85.
Thomas M Davis Melanie E Shields Qian Zhang Denise Tombolato-Terzić Jeffrey L Bennetzen Ana C Pontaroli Hao Wang Qin Yao Phillip SanMiguel Kevin M Folta 《BMC plant biology》2010,10(1):81
Background
Strawberry (Fragaria spp.) is the familiar name of a group of economically important crop plants and wild relatives that also represent an emerging system for the study of gene and genome evolution. Its small stature, rapid seed-to-seed cycle, transformability and miniscule basic genome make strawberry an attractive system to study processes related to plant physiology, development and crop production; yet it lacks substantial genomics-level resources. This report addresses this deficiency by characterizing 0.71 Mbp of gene space from a diploid species (F. vesca). The twenty large genomic tracks (30-52 kb) captured as fosmid inserts comprise gene regions with roles in flowering, disease resistance, and metabolism. 相似文献86.
目的: 验证临床受试者所完成的心肺运动试验(CPET)为最大极限运动,进一步设计完善Max试验验证CPET结果客观定量功能评估的准确性及以某特定指标的特定数值作为停止运动的标准是否可行。方法: 选择2017年9月至2019年1月在阜外医院签署知情同意书后进行CPET和Max试验受试者216例。其中正常受试者41例,因CPET峰值呼吸交换率(RER)≤1.10,或运动中心率和血压不上升,对CPET极限运动结果存在质疑的临床患者175例进行研究。其中60例已初步报告,本研究进一步扩大研究。Max试验方法:完成CPET测试后,先蹬车≥60 r/min,再施加130%峰值功率的恒定功率,鼓励受试者运动至不能坚持的极限状态。计算分析Max试验30 s的最大心率和最大摄氧量、及其与峰值心率和峰值摄氧量之间的差值和百分差值。百分差值=(Max值-峰值值)/Max值× 100%。评测标准:①若心率和摄氧量任一指标的差值百分比≤-10%(Max测试的数值低于CPET峰值数据)则定义Max试验操作失败,否则为成功;2若心率和摄氧量的差值百分比均在-10%~10%,则Max试验操作成功,证明CPET数据为极限运动,CPET 峰值相关数据较为准确;③若心率和摄氧量差值任一指标差值百分比≥10%时,则Max试验操作成功,证明CPET结果为非极限运动。结果: 病例组峰值摄氧量(L/min、ml/(min·kg)、%pred)、无氧阈(L/min、ml/(min·kg)、%pred)、峰值氧脉搏(ml/beat、%pred)、峰值RER、峰值收缩压(mmHg)、峰值运动负荷(W/min)、峰值心率(bpm)、摄氧有效性峰值平台(OUEP)(比值、%pred)低于正常组,二氧化碳通气有效性平均90 s最低值(Lowest Ve/VCO2)(比值、%pred)、二氧化碳通气效率斜率(Ve/VCO2 Slope)(比值、%pred)高于正常组(P<0.05)。所有正常组与病例组均安全无任何事件完成CPET和Max试验。216例受试者中,Max试验成功198例(91.7%),其中证明CPET为极限运动182例,为非极限运动16例;失败18例(8.3%)。结论: 在临床检查中,若对CPET结果是否为最大极限存在质疑,利用Max试验可验证CPET是否为极限运动。Max试验方法安全可行,值得进一步深入研究和临床推广应用。 相似文献
87.
Expression of interferon consensus sequence binding protein (ICSBP) is downregulated in Bcr-Abl-induced murine chronic myelogenous leukemia-like disease, and forced coexpression of ICSBP inhibits Bcr-Abl-induced myeloproliferative disorder 下载免费PDF全文
Chronic myelogenous leukemia (CML) is a clonal myeloproliferative disorder resulting from the neoplastic transformation of a hematopoietic stem cell. The majority of cases of CML are associated with the (9;22) chromosome translocation that generates the bcr-abl chimeric gene. Alpha interferon (IFN-alpha) treatment induces hematological remission and prolongs life in 75% of CML patients in the chronic phase. It has been shown that mice deficient in interferon consensus sequence binding protein (ICSBP), a member of the interferon regulatory factor family, manifest a CML-like syndrome. We have shown that expression of Bcr-Abl in bone marrow (BM) cells from 5-fluorouracil (5-FU)-treated mice by retroviral transduction efficiently induces a myeloproliferative disease in mice resembling human CML. To directly test whether icsbp can function as a tumor suppressor gene, we examined the effect of ICSBP on Bcr-Abl-induced CML-like disease using this murine model for CML. We found that expression of the ICSBP protein was significantly decreased in Bcr-Abl-induced CML-like disease. Forced coexpression of ICSBP inhibited the Bcr-Abl-induced colony formation of BM cells from 5-FU-treated mice in vitro and Bcr-Abl-induced CML-like disease in vivo. Interestingly, coexpression of ICSBP and Bcr-Abl induced a transient B-lymphoproliferative disorder in the murine model of Bcr-Abl-induced CML-like disease. Overexpression of ICSBP consistently promotes rather than inhibits Bcr-Abl-induced B lymphoproliferation in a murine model where BM cells from non-5-FU-treated donors were used, indicating that ICSBP has a specific antitumor activity toward myeloid neoplasms. We also found that overexpression of ICSBP negatively regulated normal hematopoiesis. These data provide direct evidence that ICSBP can act as a tumor suppressor that regulates normal and neoplastic proliferation of hematopoietic cells. 相似文献
88.
目的观察昼夜节律对大鼠局灶性脑缺血再灌注损伤的影响。方法将大鼠置人工光(6:00-18:00)暗(18:00-6:00)环境中,适应性饲养4周。然后将大鼠随机分为6:00组、12:00组、18:00组和24:00组,分别在相应的时间点采用线栓法制备局灶性脑缺血再灌注损伤大鼠模型。再灌注24 h后,观察昼夜节律对模型大鼠神经损伤症状,脑梗死范围,脑组织超氧化物歧化酶(SOD)活性和MDA含量,脑细胞内游离Ca2+浓度,血清中内皮素(ET)和血管内皮细胞生长因子(VEGF),以及脑组织病理变化的影响。结果各组大鼠均具有明显的神经损伤症状,其中24:00组大鼠的神经损伤症状明显轻于6:00组;6:00、12:00组大鼠脑梗死范围明显大于18:00、24:00组;18:00、24:00组大鼠SOD活性均显著高于6:00、12:00组,MDA含量均显著低于6:00组;24:00组大鼠脑细胞内游离Ca2+浓度显著低于6:00和12:00组,18:00组大鼠脑细胞内游离Ca2+浓度显著低于6:00;18:00、24:00组大鼠血清ET含量显著低于6:00组大鼠;24:00组大鼠血清VEGF含量显著低于6:00、12:00组;18:00、24:00组大鼠脑组织病理变化明显轻于6:00、12:00组。结论昼夜节律对大鼠脑缺血再灌注损伤具有显著影响。 相似文献
89.
Evolutionary and expression analysis of a MADS-box gene superfamily involved in ovule development of seeded and seedless grapevines 总被引:1,自引:0,他引:1
90.