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排序方式: 共有227条查询结果,搜索用时 244 毫秒
91.
Tarek A. Ahmed Hany M. Ibrahim Ahmed M. Samy Alaa Kaseem Mohammad T. H. Nutan Muhammad Delwar Hussain 《AAPS PharmSciTech》2014,15(3):772-780
The objective of this study was to investigate the sustained release of a hydrophilic drug, montelukast (MK), from two biodegradable polymeric drug delivery systems, in situ implant (ISI) and in situ microparticles (ISM). N-Methyl pyrrolidone (NMP), dimethyl sulfoxide (DMSO), triacetin, and ethyl acetate were selected as solvents. The release of 10% (w/v) MK from both systems containing poly-lactic-co-glycolic acid (PLGA) as the biodegradable polymer was compared. Upon contact with the aqueous medium, the PLGA in ISI and ISM systems solidified resulting in implants and microparticles, respectively. The in vitro drug release from the ISI system showed marked difference from miscible solvents (NMP and DMSO) than the partially miscible ones (triacetin and ethyl acetate), and the drug release decreased with increased PLGA concentration. In the ISM system, the initial in vitro drug release decreased with decreased ratio of polymer phase to external oil phase. In vivo studies in rats showed that ISM had slower drug release than the drug release from ISI. Also, the ISM system when compared to ISI system had significantly reduced initial burst effect. In vitro as well as the in vivo studies for both ISI and ISM systems showed sustained release of MK. The ISM system is suitable for sustained release of MK over 4-week period with a lower initial burst compared to the ISI system. Stability studies of the ISI and ISM formulations showed that MK is stable in the formulations stored at 4°C for more than 2 years. 相似文献
92.
Asfour IA El Shazly S Fayek MH Hegab HM Raouf S Moussa MA 《Biological trace element research》2006,110(1):19-32
The present study was undertaken to explore the effect of the administration of high doses of sodium selenite on apoptosis in polymorphonuclear leukocytes in patients with non-Hodgkin's lymphoma. Thirty patients with newly diagnosed non-Hodgkin's lymphoma were randomly divided into two groups. Group I was treated with chemotherapy and group II received 0.2 mg/kg/d sodium selenite in addition to chemotherapy. Flow cytometry was used for the monitoring of apoptosis on peripheral blood neutrophils at the time of diagnosis and after treatment in both groups of patients. Sodium selenite administration resulted in a significant reduction in neutrophils apoptosis (82+/-10% vs 32+/-18%, p<0.05) and this was associated with significant reduction in infection rate following chemotherapy (67% vs 20%, p<0.05). Also, significant improvement in cardiac ejection fraction was observed (62+/-4% vs 69+/-5% p<0.05). It is concluded that sodium selenite administration at the dosage chosen acts as a cytoprotective agent, alleviating side effects and immunosuppressive effects of cytotoxic chemotherapeutic agents. 相似文献
93.
El-Bestawy E El-Sokkary I Hussein H Keela AF 《Journal of industrial microbiology & biotechnology》2008,35(11):1517-1529
The main objective of the present study was to improve the quality of pulp and paper industrial wastewater of two local mills
RAKTA and El-Ahlia, Alexandria, Egypt, and to bring their pollutant contents to safe discharge levels. Quality improvement
was carried out using integrated chemical and biological treatment approaches after their optimization. Chemical treatment
(alum, lime, and ferric chloride) was followed by oxidation using hydrogen peroxide and finally biological treatment using
activated sludge (90 min for RAKTA and 60 min for El-Ahlia effluents). Chemical coagulation produced low-quality effluents,
while pH adjustment during coagulation treatment did not enhance the quality of the effluents. Maximum removal of the tested
pollutants was achieved using the integrated treatment and the pollutants recorded residual concentrations (RCs) of 34.67,
17.33, 0.13, and 0.43 mg/l and 15.0, 11.0, 0.0, and 0.13 mg/l for chemical oxygen demand (COD), biochemical oxygen demand
(BOD5), tannin and lignin, and silica in RAKTA and El-Ahlia effluents, respectively, all of which were below their maximum permissible
limits (MPLs) for the safe discharge into water courses. Specific oxygen uptake rate (SOUR) and sludge volume index (SVI)
values reflect good conditions and healthy activated sludge. Based on the previous results, optimized conditions were applied
as bench scale on the raw effluents of RAKTA and El-Ahlia via the batch chemical and the biological treatment sequences proposed.
For RAKTA effluents, the sequence was as follows: (1) coagulation with 375 mg/l FeCl3, (2) oxidation with 50 mg/l hydrogen peroxide, and (3) biological treatment using activated sludge with 2,000 mg/l initial
concentration and 90 min hydraulic retention time (HRT), while for El-Ahlia raw effluents, the sequence was (1) coagulation
with 250 mg/l FeCl3, (2) oxidation with 45 mg/l hydrogen peroxide, and (3) biological treatment using activated sludge with 2,000 mg/l initial
concentration and 60 min HRT. In conclusion, results confirmed that the application of the proposed sequential treatments
removed almost all COD, BOD5, high molecular weight compounds, and silica from RAKTA and El-Ahlia influents and produced high-quality effluents, thus
achieving the main objective of this study. 相似文献
94.
Muhammad Iqbal Chaudhary Joseph J. Adu-Gyamfi Hirofumi Saneoka Nguyen Tran Nguyen Ryuichi Suwa Shynsuke Kanai Hany A. El-Shemy David A. Lightfoot Kounosuke Fujita 《Acta Physiologiae Plantarum》2008,30(4):537-544
Phosphorous (P) fertilization is the major mineral nutrient yield determinant among legume crops. However, legume crops vary
widely in the ability to take up and use P during deficiency. The aim here was to compare P uptake and translocation, biological
nitrogen fixing ability and photosynthetic rate among mashbean (Vigna aconitifolia cv. ‘Mash-88’), mungbean (Vigna
radiata cv. ‘Moong-6601’) and soybean (Glycine max L. cv. ‘Tamahomare’) during deficiency in hydroponics. Two treatments, the withdrawal of P from the solution (P-deprivation)
and continued P at 160 μM (P sufficient) were effected at the pod initiation stage. Plants were grown for 20 days. Short-term
labeling with 32P showed the uptake and distribution of P into plant parts. Withdrawal of P from the solution reduced biomass, photosynthetic
activity, and nitrogen fixing ability in mungbean, and mashbean more than in soybean. P deprivation decreased P accumulation
more than N accumulation. The decrease was more severe in mungbean and mashbean than soybean. More P was translocated and
distributed into leaves in soybean than in mungbean and mashbean. Leaf P amount was more correlated to leaf area than to photosynthetic
rate per unit leaf area among all three legume species. The results indicate that selection for increased efficiency of P
utilization and leaf area may be used to improve leguminous crops. 相似文献
95.
Nanotubes impregnated human olfactory bulb neural stem cells promote neuronal differentiation in Trimethyltin‐induced neurodegeneration rat model 下载免费PDF全文
96.
Victoria S. Haritos Hany Ghabrial Jorma T. Ahokas Michael S. Ching 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1999,736(1-2)
A stereoselective method of analysis of the antidepressant drug doxepin (DOX, an 85:15% mixture of E–Z stereoisomers), its principal metabolites E- and Z-N-desmethyldoxepin (desDOX) and ring-hydroxylated metabolites in microsomal incubation mixtures is described. DOX and its metabolites were extracted from alkalinised incubation mixtures by either: 9:1 hexane–propan-2-ol (method 1) or 1:1 hexane–dichloromethane (method 2), derivatised with trifluoroacetic anhydride and analysed by GC–MS with selected ion monitoring. Both methods were suitable for the analysis of individual desDOX isomers as indicated by correlation coefficients of ≥0.999 for calibration curves constructed between 50 and 2500 nM, and good within-day precision at 125 nM (C.V. ≤14%) and 1000 nM (C.V. ≤8%). Method 1, however, was unsuitable for the analysis of ring-hydroxylated metabolites of DOX, whereas the hydroxylated metabolites of E-DOX and E-desDOX (generated in situ) were extracted by method 2 with a C.V. of ca. 13%. This is the first assay method that permits the simultaneous measurement of desDOX and hydroxylated metabolites of DOX in microsomal mixtures. 相似文献
97.
Sherin Y. Naiem Ayman E. Badran Mohamed S. Boghdady Bandar S. Aljuaid Ahmed M. El-Shehawi Heba M. Salem Amira M. El-Tahan Hany E. Ismail 《Saudi Journal of Biological Sciences》2022,29(4):2645-2655
The current experiment was adopted during the summer 2018, fall 2018/2019 and summer 2019 respectively at the Experimental Farm of Baloza station, Desert Research Center. North Sinai Governorate, Egypt to study the effect of different doses of irradiation (0, 20, 30 and 40 Gy), three irrigation levels (100, 80 and 60% field capacity on growth, yield and its quality of some potato cultivars (Spunta, Cara, Caruso and Hermes). Treated Spunta cultivar pre planting with 20 (Gy) and irrigated with 80% field capacity was the best treatment for increasing number of aerial stem/plants, leaf area, total chlorophyll in leaves, average tuber weight, and total yield/fed. Hermes cultivar with 20 (Gy) and irrigation level of 80% was the best for increasing dry matter content in tuber in both mutagenic generations. 相似文献
98.
Florina Moldovan Houda Benanni Jean Fiet Olivier Cussenot Jacques Dumas Christian Darbord Hany R. Soliman 《In vitro cellular & developmental biology. Animal》1996,32(1):16-23
Summary The plasmid pMK16 containing-SV40 replicated origin defective gene was efficiently introduced into early-passage human umbilical
vein endothelial cells (HUVEC) using positively charged liposomes. The resulting cell line acquired an almost infinite lifespan,
was morphologically unchanged, expressed SV40-antigen, and coexpressed von Willebrand factor (vWF), tissue plasminogen activator
(t-PA), plasminogen activator inhibitor-1 (PAI-1), angiotensin conversion enzyme (ACE), and endothelin converting enzyme (ECE).
In addition, these are the first immortalized human endothelial cells, to our knowledge, that biosynthesized and secreted
interleukins (IL-1β and IL-6) in both a constitutive and regulated fashion and endothelin-1 (ET-1), the most potent vasoactive
peptide, which has been suggested to be implicated in the pathogenesis of hypertension. Interestingly enough, both of the
immortalized cells and the early-passage HUVEC from which the immortalized cells were obtained biosynthesized and secreted
the same levels of ET-1 suggesting full maintenance of its biosynthetic pathway including the presence of active ECE, which
cleaves big endothelin-1 (big-ET-1) to ET-1 and regulation factors. Moreover, the immortalized cells retained the ability
to express the functional specific amino acid Na+-independent system Y+ transporter, which mediates L-arginine transport into endothelial cells from which endothelium-derived relaxing factor (EDRF,
nitric oxide) is formed via the action of nitric oxide-synthase. Obtaining these immortalized human endothelial cells without
alteration of the differentiated characteristics constitutes a useful model: (a) to study ET-1 secretion, gene regulation,
and human ECE, which may be an important therapeutic target in disease conditions in which ET-1 is to be implicated; (b) to
study L-arginine transport, which is a key step in the formation of EDRF; (c) to study IL-1β and IL-6 secretions, and gene
regulations; (d) to substitute large quantities of HUVEC; and, finally, (e) to reproduce, starting with different primary
endothelial cells both from human and animal origin. 相似文献
99.
E. S. Lashen 《Applied microbiology》1971,21(4):771-773
Bacteriostasis of treated fabric was evaluated directly by a colony count made on the treated fabric after impregnation with seeded agar containing triphenyltetrazolium chloride and incubation in a horizontal, suspended position in a petri dish. 相似文献
100.
Wagdy M. Eldehna Dina H. EL-Naggar Ahmed R. Hamed Hany S. Ibrahim Hazem A. Ghabbour Hatem A. Abdel-Aziz 《Journal of enzyme inhibition and medicinal chemistry》2018,33(1):309-318
Triple-negative breast cancer (TNBC) is a highly aggressive malignancy with limited treatment options due to its heterogeneity and the lack of well-defined molecular targets. In our endeavour towards the development of novel anti-TNBC agents, herein we report a one-pot three-component synthesis of novel spirooxindoles 6a–p, and evaluation of their potential anti-proliferative activity towards TNBC MDA-MB-231 cells. Spirooxindoles 6a, 6e and 6i emerged as the most potent analogues with IC50 =?6.70, 6.40 and 6.70?µM, respectively. Compounds 6a and 6e induced apoptosis in MDA-MB-231 cells, as evidenced by the up-regulation of the Bax and down-regulation of the Bcl-2, besides boosting caspase-3 levels. Additionally, 6e displayed significant increase in the percent of annexin V-FITC positive apoptotic cells from 1.34 to 44%. Furthermore, spirooxindoles 6e and 6i displayed good inhibitory activity against EGFR (IC50 =?120 and 150?nM, respectively). Collectively, these data demonstrated that 6e might be a potential lead compound for the development of effective anti-TNBC agents. 相似文献