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Gröschel B Kaufmann A Cinatl J Doerr HW Cinatl J 《Nucleosides, nucleotides & nucleic acids》2001,20(4-7):1433-1437
Continuous cultivation of T-lymphoid C8166 cells in the presence of pharmacological relevant concentration of cytarabine (Ara-C) results in significantly decreased expression of CD4 and CXCR4 molecules, the major cellular receptor and co-receptor of T-lymphotropic HIV-1 isolates. This change in receptor expression leads to decreased susceptibility of Ara-C resistant cells to HIV-1 infection demonstrated by reduced binding and penetration of HI-virus. 相似文献
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Benjamin Rothé Lucia Leal-Esteban Florian Bernet Séverine Urfer Nicholas Doerr Thomas Weimbs Justyna Iwaszkiewicz Daniel B. Constam 《Molecular and cellular biology》2015,35(19):3339-3353
Loss of the RNA-binding protein Bicaudal-C (Bicc1) provokes renal and pancreatic cysts as well as ectopic Wnt/β-catenin signaling during visceral left-right patterning. Renal cysts are linked to defective silencing of Bicc1 target mRNAs, including adenylate cyclase 6 (AC6). RNA binding of Bicc1 is mediated by N-terminal KH domains, whereas a C-terminal sterile alpha motif (SAM) self-polymerizes in vitro and localizes Bicc1 in cytoplasmic foci in vivo. To assess a role for multimerization in silencing, we conducted structure modeling and then mutated the SAM domain residues which in this model were predicted to polymerize Bicc1 in a left-handed helix. We show that a SAM-SAM interface concentrates Bicc1 in cytoplasmic clusters to specifically localize and silence bound mRNA. In addition, defective polymerization decreases Bicc1 stability and thus indirectly attenuates inhibition of Dishevelled 2 in the Wnt/β-catenin pathway. Importantly, aberrant C-terminal extension of the SAM domain in bpk mutant Bicc1 phenocopied these defects. We conclude that polymerization is a novel disease-relevant mechanism both to stabilize Bicc1 and to present associated mRNAs in specific silencing platforms. 相似文献
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Doerr A 《Nature methods》2007,4(5):381
Researchers demonstrate how microfluidics can be used to both synthesize millions of differentially encoded particles for multiplexed in vitro biological detection assays, as well as to decode their identities. 相似文献
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Pyrogenic organic matter production from wildfires: a missing sink in the global carbon cycle 下载免费PDF全文
Cristina Santín Stefan H. Doerr Caroline M. Preston Gil González‐Rodríguez 《Global Change Biology》2015,21(4):1621-1633
Wildfires release substantial quantities of carbon (C) into the atmosphere but they also convert part of the burnt biomass into pyrogenic organic matter (PyOM). This is richer in C and, overall, more resistant to environmental degradation than the original biomass, and, therefore, PyOM production is an efficient mechanism for C sequestration. The magnitude of this C sink, however, remains poorly quantified, and current production estimates, which suggest that ~1‐5% of the C affected by fire is converted to PyOM, are based on incomplete inventories. Here, we quantify, for the first time, the complete range of PyOM components found in‐situ immediately after a typical boreal forest fire. We utilized an experimental high‐intensity crown fire in a jack pine forest (Pinus banksiana) and carried out a detailed pre‐ and postfire inventory and quantification of all fuel components, and the PyOM (i.e., all visually charred, blackened materials) produced in each of them. Our results show that, overall, 27.6% of the C affected by fire was retained in PyOM (4.8 ± 0.8 t C ha?1), rather than emitted to the atmosphere (12.6 ± 4.5 t C ha?1). The conversion rates varied substantially between fuel components. For down wood and bark, over half of the C affected was converted to PyOM, whereas for forest floor it was only one quarter, and less than a tenth for needles. If the overall conversion rate found here were applicable to boreal wildfire in general, it would translate into a PyOM production of ~100 Tg C yr?1 by wildfire in the global boreal regions, more than five times the amount estimated previously. Our findings suggest that PyOM production from boreal wildfires, and potentially also from other fire‐prone ecosystems, may have been underestimated and that its quantitative importance as a C sink warrants its inclusion in the global C budget estimates. 相似文献
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Neriko Musha Doerr 《Anthropology & education quarterly》2004,35(2):233-253
In this article, I offer a new interpretation of non-Mäori parents' claim that a Mäori/English bilingual school unit in Aotearoa/New Zealand is separatist. I show that while some use such a claim to defend monoculturalism and white hegemony, others use it to come to terms with Aotearoa/New Zealand's postcolonial nationhood, globalization, and a changing meaning of "ethnic harmony." I suggest collaborations to rearticulate the latter parents' concerns, thereby creating new alliances to support minority education. 相似文献
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Jindrich Cinatl Jaroslav Cinatl Holger Rabenau Jens Rapp Bernhardt Kornhuber Hans-Wilhelm Doerr 《Cell biology international》1993,17(9):885-895
A protein-free chemically defined medium designated PFEK-1 was developed for culture of VERO cells on polyvinyl formal (PVF) culture surface without serum or other macromolecular supplements. VERO cells proliferated in PFEK-1 medium on PVF surface to a similar extent as cells in serum-supplemented medium without previous adaptation from serum-containing conditions. The protein-free culture infected with coxsackievirus B4, herpes simplex virus types 1 and 2, measles virus and poliovirus types 1, 2 and 3 developed viral titers comparable to those found in conventionally grown cells. The results demonstrated that VERO cells in protein-free culture provide a sensitive substrate for the production of human pathogenic viruses which are not contaminated by serum or other protein factors usually added to a culture medium. 相似文献