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141.
Rüter J Hoffmann T Demuth HU Moschansky P Klapp BF Hildebrandt M 《Biological chemistry》2004,385(6):537-541
We assessed changes of the enzyme dipeptidyl peptidase IV (DPP IV, CD26) in the context of leptin or leptin receptor deficiency. C57BL/6 mice, Leptin-deficient mice (ob/ob mice, B6.V-Lep) and Leptin-receptor-deficient mice (db/db mice, B6.Cg-m+/+Lepr) were infected with B. Calmette-Guerin (BCG) and sacrificed three days later. DPP IV activity in serum was higher in ob/ob mice and in db/db mice than in wild-type mice. The expression of DPP IV/CD26 on splenocytes was higher in ob/ob mice than in wild-type animals, and lower in db/db mice, and decreased upon stimulation with BCG in ob/ob mice only. Several T cell antigens including CTLA-4 were expressed aberrantly in ob/ob and in db/db mice. Our observations provide evidence for a relationship between DPP IV and leptin. 相似文献
142.
Rüter J Hoffmann T Heiser U Demuth HU Arck PC Klapp BF Hildebrandt M 《Cellular immunology》2002,220(2):150-156
Inhibition of DPP IV has been shown to abrogate the stress-related increase in murine abortions and a concomitant increase in gamma-interferon. The aim of the present study was to investigate a potential impact of the DPP IV inhibitor Isoleucine Cyanopyrrolidide on the expression of surface antigens involved in T-cell responses. DBA/2-mated CBA mice were stressed on day 5.5 of pregnancy and received injections of a DPP IV inhibitor. On day 13 of gestation, the animals were sacrificed and the percentage of abortions was determined. As shown before, stress failed to boost the abortion rate in mice receiving the DPP IV inhibitor. In stressed animals, a lower surface density of CTLA-4 on decidual CD26-positive lymphocytes was observed than in non-stressed animals. Inhibition of DPP IV restored CTLA-4 surface density to normal and decreased surface expression of CD26 and CD28 on decidual lymphocytes irrespective of stress exposure. These observations suggest that a modulation of T-cell surface antigens expression due to inhibition of DPP IV activity may contribute to the potent anti-abortogenic effect observed here. 相似文献
143.
144.
The nucleotide sequence and derived amino acid sequence of cDNA coding for mouse carbonic anhydrase II 总被引:9,自引:0,他引:9
The nucleotide sequence of a clone containing mouse carbonic anhydrase (CA) cDNA in pBR322 has been determined. The cloned cDNA contains all of the coding region except for nucleotides specifying the first eight amino acids, and all of the 3' noncoding region, which consists of 700 nucleotides. A cDNA clone was identified which contains an additional 54 bp at the 5' end, so that the complete amino acid sequence of mouse CA could be deduced. This sequence showed a 73-81% homology with other mammalian CA form II isozymes, 56-63% with form I isozymes, and 52-56% with form III isozymes. By examination of the amino acids which are unique and invariant for each isozyme, the mouse amino acid sequence was found to contain 16 of the 23 residues that are unique and invariant to mammalian CA form II isozymes, but only one or no residue for forms I and III, respectively. 相似文献
145.
Echolocation behavior of rufous horseshoe bats hunting for insects in the flycatcher-style 总被引:1,自引:0,他引:1
Hans-Ulrich Schnitzler Heidi Hackbarth Ute Heilmann Horst Herbert 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1985,157(1):39-46
Summary The echolocation behavior ofRhinolophus rouxi when waiting for insects on a perch and when pursuing them in short hunting flights is described. It reveals that flycatcher-style hunting is one foraging strategy utilized by this species. It also suggests that bats use fluttering target information to classify their prey. 相似文献
146.
Hans-Ulrich Thiele 《Oecologia》1969,3(2):227-229
Summary Carabid beetles of forest habitats are mostly hygrophilous and darkpreferring species. Nearly all of them are nocturnal, and the majority are autumn breeders. Most carabids from open field habitats are xerophilous, light-preferring and diurnal species, or they are indifferent to humidity and light and can be active by day and night. This group includes markedly more spring breeders than the forest species. The adaptative value of the observed correlations is discussed. 相似文献
147.
148.
Ultramicroscopy is a powerful tool to reveal detailed three-dimensional structures of large microscopical objects. Using high magnification, we observed that formalin induces fluorescence more in extra-cellular space and stains cellular structures negatively, rendering cells as dark objects in front of a bright background. Here, we show this effect on a three-dimensional image stack of a hippocampus sample, focusing on the CA1 region. This method, called FIF-Ultramicroscopy, allows for the three-dimensional observation of cellular structures in various tissue types without complicated staining techniques. 相似文献
149.
Axel Newe Stefan Wimmer Antje Neubert Linda Becker Hans-Ulrich Prokosch Matthias W. Beckmann Rainer Fietkau Christian Forster Markus Friedrich Neurath Georg Schett Thomas Ganslandt 《PloS one》2015,10(8)
Background
The analysis of electronic health records for an automated detection of adverse drug reactions is an approach to solve the problems that arise from traditional methods like spontaneous reporting or manual chart review. Algorithms addressing this task should be modeled on the criteria for a standardized case causality assessment defined by the World Health Organization. One of these criteria is the temporal relationship between drug intake and the occurrence of a reaction or a laboratory test abnormality. Appropriate data that would allow for developing or validating related algorithms is not publicly available, though.Methods
In order to provide such data, retrospective routine data of drug administrations and temporally corresponding laboratory observations from a university clinic were extracted, transformed and evaluated by experts in terms of a reasonable time relationship between drug administration and lab value alteration.Result
The result is a data corpus of 400 episodes of normalized laboratory parameter values in temporal context with drug administrations. Each episode has been manually classified whether it contains data that might indicate a temporal correlation between the drug administration and the change of the lab value course, whether such a change is not observable or whether a decision between those two options is not possible due to the data. In addition, each episode has been assigned a concordance value which indicates how difficult it is to assess. This is the first open data corpus of a computable ground truth of temporal correlations between drug administration and lab value alterations.Discussion
The main purpose of this data corpus is the provision of data for further research and the provision of a ground truth which allows for comparing the outcome of other assessments of this data with the outcome of assessments made by human experts. It can serve as a contribution towards systematic, computerized ADR detection in retrospective data. With this lab value curve data as a basis, algorithms for detecting temporal relationships can be developed, and with the classification made by human experts, these algorithms can immediately be validated. Due to the normalization of the lab value data, it allows for a generic approach rather than for specific or solitary drug/lab value combinations. 相似文献150.
Martin K?chele Anita M. Hennige Jürgen Machann Anja Hieronimus Apostolia Lamprinou Fausto Machicao Fritz Schick Andreas Fritsche Norbert Stefan Bernd Nürnberg Hans-Ulrich H?ring Harald Staiger 《PloS one》2015,10(12)