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81.
82.
Beatriz Sierra Ana B. Perez Gissel Garcia Eglys Aguirre Florian Kern Hans-Dieter Volk 《Cellular immunology》2010,262(2):134-140
Increased serum levels of cytokines released by cells of the immune response have been detected in patients suffering from dengue disease. Likewise, secondary infections by a different dengue virus serotype result in a highest risk of development of the severe dengue disease. Both findings suggest that the memory immune response is one of the key players in the pathogenesis of this disease. Here we take advantage of the particular Cuban epidemiological situation in dengue to analyze a broad spectrum of cell-mediated immune response mediators at mRNA and protein level. Evidences for a regulatory immune pattern in homologous (TGF-β, IL-10) vs. pro-inflammatory pattern (IFN-γ, TNF-α) in heterologous dengue virus re-challenge were found, suggesting a possible association with the higher incidence of severe dengue cases in the latter case. 相似文献
83.
Gangliosides are not essential for influenza virus infection 总被引:1,自引:0,他引:1
Matrosovich M Suzuki T Hirabayashi Y Garten W Webster RG Klenk HD 《Glycoconjugate journal》2006,23(1-2):107-113
Sialic acid is known to be an essential part of influenza virus receptors, but the specific identity of the receptor molecules
on target cells is still not defined. In particular, the relative roles played by cellular sialylglycoproteins and gangliosides
in virus entry into target cells remain unclear. To test whether gangliosides are essential for virus infection, we used the
GM-95 mutant cell line of mouse B16 melanoma which lacks synthesis of major glycosphingolipids including gangliosides. We
found that GM-95 cells grown in serum-containing medium harboured substantial amounts of ganglioside receptors for influenza
virus due to incorporation of serum gangliosides. To obtain ganglioside-free cells, we adapted GM-95 cells to growth in defined
serum-free (sf) medium. Ganglioside-free GM-95-sf cells could be infected by avian and human influenza A viruses and produced
infectious virus progeny demonstrating that gangliosides were neither absolutely necessary for the early nor for the late
stages of the infection. However, sensitivity of the GM-95-sf cells to the viruses was 2–4 times lower than that of the ganglioside-containing
parent cell line. Further studies are needed to specify whether this effect was due to the lack of gangliosides, neutral glycosphingolipids,
or other effects. 相似文献
84.
Nanogomphodon wildi n. gen., n. sp. is based on a tiny lower postcanine tooth from the lower Lettenkeuper (Lower Keuper or Erfurt Formation;
Ladinian) of Michelbach an der Bilz (Baden-Württemberg). It represents the first record of a traversodont cynodont from the
Middle Triassic of Europe and exhibits a distinctive combination of dental features. Along with recent discoveries of other
traversodont taxa from the Upper Triassic of eastern North America,Nanogomphodon indicates the existence of a distinct lineage of these cynodonts in the Northern Hemisphere.
相似文献
85.
Schmidt-Bleek K Schell H Schulz N Hoff P Perka C Buttgereit F Volk HD Lienau J Duda GN 《Cell and tissue research》2012,347(3):567-573
Bone healing commences with an inflammatory reaction which initiates the regenerative healing process leading in the end to
reconstitution of bone. An unbalanced immune reaction during this early bone healing phase is hypothesized to disturb the
healing cascade in a way that delays bone healing and jeopardizes the successful healing outcome. The immune cell composition
and expression pattern of angiogenic factors were investigated in a sheep bone osteotomy model and compared to a mechanically-induced
impaired/delayed bone healing group. In the impaired/delayed healing group, significantly higher T cell percentages were present
in the bone hematoma and the bone marrow adjacent to the osteotomy gap when compared to the normal healing group. This was
mirrored in the higher cytotoxic T cell percentage detected under delayed bone healing conditions indicating longer pro-inflammatory
processes. The highly activated periosteum adjourning the osteotomy gap showed lower expression of hematopoietic stem cell
markers and angiogenic factors such as heme oxygenase and vascular endothelial growth factor. This indicates a deferred revascularization
of the injured area due to ongoing pro-inflammatory processes in the delayed healing group. Results from this study suggest
that there are unfavorable immune cells and factors participating in the initial healing phase. In conclusion, identifying
beneficial aspects may lead to promising therapeutical approaches that might benefit further by eliminating the unfavorable
factors. 相似文献
86.
We assessed viability of 18 strains of filamentous ectomycorrhizal and saprotrophic basid-iomycetes and ascomycetes after cryopreservation with a novel technique based on charcoal filter paper strips (CFS). The results indicate that axenic fungal cultures grown on CFS recovered from freezing within a few days, even though none survived cryopreservation by the conventional straw method. Fungal growth on CFS was more vigorous, with morphological differentiations such as rhizomorphs and an increased amount of aerial mycelia compared to the unamended culture media. Accordingly CFS allows the cryopreservation of a wide range of rare and important ectomycorrhizal and saprotrophic fungi, which hitherto were difficult to revive from liquid nitrogen storage with the conventional and widely applied straw technique. 相似文献
87.
I Anderson E Saunders A Lapidus M Nolan S Lucas H Tice TG Del Rio JF Cheng C Han R Tapia LA Goodwin S Pitluck K Liolios K Mavromatis I Pagani N Ivanova N Mikhailova A Pati A Chen K Palaniappan M Land L Hauser CD Jeffries YJ Chang EM Brambilla M Rohde S Spring M Göker JC Detter T Woyke J Bristow JA Eisen V Markowitz P Hugenholtz NC Kyrpides HP Klenk 《Standards in genomic sciences》2012,6(2):155-164
Thermodesulfatator indicus Moussard et al. 2004 is a member of the Thermodesulfobacteriaceae, a family in the phylum Thermodesulfobacteria that is currently poorly characterized at the genome level. Members of this phylum are of interest because they represent a distinct, deep-branching, Gram-negative lineage. T. indicus is an anaerobic, thermophilic, chemolithoautotrophic sulfate reducer isolated from a deep-sea hydrothermal vent. Here we describe the features of this organism, together with the complete genome sequence, and annotation. The 2,322,224 bp long chromosome with its 2,233 protein-coding and 58 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献
88.
A Copeland A Zeytun M Yassawong M Nolan S Lucas N Hammon S Deshpande JF Cheng C Han R Tapia LA Goodwin S Pitluck K Mavromatis K Liolios I Pagani N Ivanova N Mikhailova A Pati A Chen K Palaniappan M Land L Hauser CD Jeffries EM Brambilla M Rohde J Sikorski R Pukall M Göker JC Detter T Woyke J Bristow JA Eisen V Markowitz P Hugenholtz NC Kyrpides HP Klenk A Lapidus 《Standards in genomic sciences》2012,6(2):240-250
Deinococcus proteolyticus (ex Kobatake et al. 1973) Brook and Murray 1981 is one of currently 47 species in the genus Deinococcus within the family Deinococcaceae. Strain MRP(T) was isolated from feces of Lama glama and possesses extreme radiation resistance, a trait is shares with various other species of the genus Deinococcus, with D. proteolyticus being resistant up to 1.5 Mrad of gamma radiation. Strain MRP(T) is of further interest for its carotenoid pigment. The genome presented here is only the fifth completed genome sequence of a member of the genus Deinococcus (and the forth type strain) to be published, and will hopefully contribute to a better understanding of how members of this genus adapted to high gamma- or UV ionizing-radiation. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 2,886,836 bp long genome with its four large plasmids of lengths 97 kbp, 132 kbp, 196 kbp and 315 kbp harbors 2,741 protein-coding and 58 RNA genes and is a part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献
89.
B Abt C Han C Scheuner M Lu A Lapidus M Nolan S Lucas N Hammon S Deshpande JF Cheng R Tapia LA Goodwin S Pitluck K Liolios I Pagani N Ivanova K Mavromatis N Mikhailova M Huntemann A Pati A Chen K Palaniappan M Land L Hauser EM Brambilla M Rohde S Spring S Gronow M Göker T Woyke J Bristow JA Eisen V Markowitz P Hugenholtz NC Kyrpides HP Klenk JC Detter 《Standards in genomic sciences》2012,6(2):194-209
Spirochaeta coccoides Dröge et al. 2006 is a member of the genus Spirochaeta Ehrenberg 1835, one of the oldest named genera within the Bacteria. S. coccoides is an obligately anaerobic, Gram-negative, non-motile, spherical bacterium that was isolated from the hindgut contents of the termite Neotermes castaneus. The species is of interest because it may play an important role in the digestion of breakdown products from cellulose and hemicellulose in the termite gut. Here we provide a taxonomic re-evaluation for strain SPN1T, and based on physiological and genomic characteristics, we propose its reclassification as a novel species in the genus Sphaerochaeta, a recently published sister group of the Spirochaeta. The 2,227,296 bp long genome of strain SPN1T with its 1,866 protein-coding and 58 RNA genes is a part of the Genomic
Encyclopedia of
Bacteria and
Archaea project. 相似文献
90.
Inhibition of dendritic cell maturation and function is independent of heme oxygenase 1 but requires the activation of STAT3 总被引:1,自引:0,他引:1
Mashreghi MF Klemz R Knosalla IS Gerstmayer B Janssen U Buelow R Jozkowicz A Dulak J Volk HD Kotsch K 《Journal of immunology (Baltimore, Md. : 1950)》2008,180(12):7919-7930
The induction of heme oxygenase 1 (HO-1) by a single treatment with cobalt protoporphyrin (CoPPIX) protects against inflammatory liver failure and ischemia reperfusion injury after allotransplantation. In this context, the HO-1-mediated inhibition of donor-derived dendritic cell maturation and migration is discussed as one of the key events of graft protection. To investigate the poorly understood mechanism of CoPPIX-induced HO-1 activity in more detail, we performed gene expression analysis in murine liver, revealing the up-regulation of STAT3 after CoPPIX treatment. By using wild-type and HO-1-deficient dendritic cells we demonstrated that LPS-induced maturation is dependent on STAT3 phosphorylation and independent of HO-1 activity. In summary, our observations revise our understanding of the anti-inflammatory properties of HO-1 and highlight the immunomodulatory capacity of STAT3, which might be of further interest for targeting undesired immune responses, including ischemia reperfusion injury. 相似文献