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61.
C. E. Carty K. J. Hofmann P. M. Keller M. A. Polokoff R. J. Lynch B. J. Keech R. J. Gould R. Z. Maigetter L. D. Schultz 《Biotechnology letters》1990,12(12):879-884
Summary The -mating factor pre-pro-leader sequence under the regulation of theGAL10 promoter was used to direct the secretion of echistatin by recombinant yeasts. Optimization of the culture medium and host strain increased the productivity of shake flask cultures twenty-fold to 8 mg/L. In fermentors, the production of echistatin was greater than 40 mg/L. 相似文献
62.
The details of spermatogenesis and spermiogenesis are described forOphryotrocha puerilis. The ultrastructure of mature sperm is shown forO. puerilis, O. hartmanni, O. gracilis, O. diadema, O. labronica, andO. notoglandulata. Clusters of sixteen cells each are proliferated by two stem cells in each setigerous segment ofO. puerilis representing the very early stages of both oogenesis and spermatogenesis. In each spermatocyte-I cluster, the cells are interconnected
by cytoplasmic bridges. Early, clusters are enveloped by peritoneal sheath cells. These transient gonad walls break down prior
to meiosis. The meiotic processes may start in the clusters with the cells still interconnected, or during breakdown of the
original cluster, giving rise to smaller subclusters of both spermatocytes I and spermatocytes II with various numbers of
cells. Finally, spermatid tetrads are present. As spermiogenesis progresses, the tetrads disintegrate. Golgi vesicles in both
spermatocytes and spermatids contain electron-dense material, presumably preacrosomal. The acrosome is formed by such vesicles.
In the six species studied here, the acrosomes appear to be of a similar overall structure but are of different shape. Centrioles
are usually located beneath the acrosome. The distal centriole forms the basal body of a flagellum-like cytoplasmic process.
The microtubules of these flagellar equivalents do not show a normal ciliar arrangement. The flagellar equivalent appears
to be non-motile. InO. hartmanni and inO. notoglandulata, a flagellar equivalent is missing. Microtubules originating from the proximal end of the distal centriole stretch to the
nuclear envelope. This feature appears to be especially conspicuous inO. puerilis and inO. labronica. InO. labronica and inO. notoglandulata, bundles of microtubules paralleling the cell perimeter appear to stabilise the sperm. Various numbers of mitochondria are
either randomly distributed around the nucleus or accumulate on one side, often directly under the acrosome.
Parts of the present paper were presented at the 2nd International Polychaete Conference, Copenhagen 1986 and at the 3rd International Polychaete Conference, Long Beach, Ca. 1989. 相似文献
63.
The PROSITE database consists of biologically significant patterns and profiles formulated in such a way that with appropriate computational tools it can help to determine to which known family of proteins (if any) a new sequence belongs or which known domain(s) it contains. 相似文献
64.
G Schumann I Zündorf J Hofmann R Marschalek T Dingermann 《Molecular and cellular biology》1994,14(5):3074-3084
65.
Abstract: Derivatives of the lac promoter (tac, pac, rac) belong to the strongest bacterial promoters which are frequently used for the induced overexpression of foreign genes in Escherichia coli . However, their use in fermentation processes is strongly restricted because of the high cost of the inducer iso-propyl-β-D-thiogalactopyranoside (IPTG). The aim of this work was to investigate the possibility of using lac-derived promoters in high cell density processes resulting in a high yield of the induced recombinant protein if glucose is the main carbon and energy source. Lactose is tested as inducer of the main antigenic coat protein (VP1) of the foot and mouth disease (FMD) virus in a T7-RNA polymerase expression system. It was shown that lactose is able to induce the expression of the recombinant gene to an amount of the VP1 protein corresponding to 20% of the total cell protein. 相似文献
66.
67.
The impact of organic nutrients and massive addition of bacteria was followed in lake water mesocosms in a eutrophic lake. Increased DOM initiated a sequence of trophic responses indicated by rapid increases in bacterioplankton, protozoa, and algal biomass. The populations of Keratella cochlearis and Keratella quadrata showed a distinct response by rapid increase in birth rate followed by maxima of production and abundance. This succession clearly reflected the trophic position of these rotifer populations in the food chain. A reverse response was observed in Conochilus unicornis. 相似文献
68.
69.
The enormous heterogeneity of the surface protein designated CD44 is in part due to posttranslational modification, and in part due to differential splicing. Alternative splicing occurs within one particular region encoding the extracellular portion of the protein. Comparison of various cDNA clones with different 'inserts' in this variable region with sequences of genomic clones from the mouse has revealed the existence of at least ten exons from which sequences are chosen by alternative splicing. Various combinations of these exons account for the tremendous heterogeneity of CD44 molecules expressed in different tissues, and in progressing tumor cells. The existence of different isoforms of CD44 suggests a broad spectrum of yet unknown physiologic functions. 相似文献
70.
Markus M. Nöthen Thomas Eggermann Jeanette Erdmann Bernd Eiben Dieter Hofmann Peter Propping Gesa Schwanitz 《Human genetics》1993,92(4):347-349
The parental origin of the extra chromosome in trisomy 18 was traced in 30 informative families using highly polymorphic (CA) repeats mapped on the long arm of chromosome 18. Proband DNA was recovered from slides of chromosome preparations in 28 cases and from paraffin-embedded tissues in two cases. The extra chromosome was found to be of maternal origin in 26 cases (86.7%), and paternal origin in 4 cases (13.3%). 相似文献