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991.
ABSTRACT: BACKGROUND: The Cucurbitaceae genus Trichosanthes comprises 90-100 species that occur from India to Japan and southeast to Australia and Fiji. Most species have large white or pale yellow petals with conspicuously fringed margins, the fringes sometimes several cm long. Pollination is usually by hawkmoths. Previous molecular data for a small number of species suggested that a monophyletic Trichosanthes might include the Asian genera Gymnopetalum (four species, lacking long petal fringes) and Hodgsonia (two species with petals fringed). Here we test these groups' relationships using a species sampling of c. 60% and 4759 nucleotides of nuclear and plastid DNA. To infer the time and direction of the geographic expansion of the Trichosanthes clade we employ molecular clock dating and statistical biogeographic reconstruction, and we also address the gain or loss of petal fringes. RESULTS: Trichosanthes is monophyletic as long as it includes Gymnopetalum, which itself is polyphyletic. The closest relative of Trichosanthes appears to be the sponge gourds, Luffa, while Hodgsonia is more distantly related. Of six morphology-based sections in Trichosanthes with more than one species, three are supported by the molecular results; two new sections appear warranted. Molecular dating and biogeographic analyses suggest an Oligocene origin of Trichosanthes in Eurasia or East Asia, followed by diversification and spread throughout the Malesian biogeographic region and into the Australian continent. CONCLUSIONS: Long-fringed corollas evolved independently in Hodgsonia and Trichosanthes, followed by two losses in the latter coincident with shifts to other pollinators but not with long-distance dispersal events. Together with the Caribbean Linnaeosicyos, the Madagascan Ampelosicyos and the tropical African Telfairia, these cucurbit lineages represent an ideal system for more detailed studies of the evolution and function of petal fringes in plant-pollinator mutualisms. 相似文献
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Hermann Brugger 《CMAJ》2009,180(5):491-492
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Pathogenic and non-pathogenic isolates of Streptococcus suis type 2 were screened to determine whether differences in superoxide dismutase (SOD) synthesis could explain the observed differences in their pathogenicity and intracellular fate in macrophages. A single band of SOD activity of similar Rf value was visualised in PAGE gels in all isolates and inhibition studies suggested that the cofactor present was manganese. There was no correlation between specific SOD activity and virulence. It is unlikely, therefore, that SOD produced by S. suis type 2 mediates intracellular survival of pathogenic isolates in macrophages. 相似文献
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Two alleles of the single-copy chalcone synthase gene in parsley differ by a transposon-like element 总被引:18,自引:0,他引:18
Anette Hermann Wolfgang Schulz Klaus Hahlbrock 《Molecular & general genetics : MGG》1988,212(1):93-98
We have analysed three nearly full-length cDNAs complementary to mRNAs encoding two PR1 (pathogenesis-related, class 1) proteins in parsley (Petroselinum crispum). Furthermore, one selected genomic clone containing the PcPR1-1 gene was investigated in detail. The structural organization and possible regulatory elements in the 5' flanking region of this gene are presented. In situ RNA hybridization in fungus-infected parsley leaf tissue demonstrated rapid and massive PR1 mRNA accumulation around infection sites. 相似文献
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