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71.
Role of lectins in plant--microorganism interactions. IV. Ultrastructural localization of soybean lectin binding sites of Rhizobium japonicum 总被引:13,自引:0,他引:13
H E Calvert M Lalonde T V Bhuvaneswari W D Bauer 《Canadian journal of microbiology》1978,24(7):785-793
The binding of purified, ferritin-labeled soybean seed lectin to the cell surfaces of Rhizobium japonicum 31 lb 138 has been examined by whole mount, thin section, and freeze-etch electron microscopy. The ferritin-labeled lectin binds in a biochemically specific manner to the capsular material of this bacterium. The lectin does not bind to the outer membranes of the cells or to flagella. Labeled lectin binds to sites throughout the capsular structure, although the density of labeling is somewhat greater on the outer surface of the capsule. Some cells appear to be partially encapsulated. Preservation of the capsular material proved difficult, and methods for retaining most of the capsular material were developed. 相似文献
72.
73.
M P Heyn P J Bauer N A Dencher 《Biochemical and biophysical research communications》1975,67(3):897-903
The polyamine and ribosome contents during the logarithmic phase of growth were much higher than those in the late logarithmic or stationary phase of growth. On the other hand, the Mg2+ content did not vary markedly throughout the different growth phases. These data, together with the results of a previous communication (1), suggest that increased polyamines during the logarithmic phase of growth may play significant roles not only in the neutralization of the negative charges of increased ribosomes but also in the increase of the velocity of polypeptide chain elongation by binding to the ribosomes. The polyphenylalanine synthetic activity of ribosomes from the logarithmic phase of growth was higher than that of ribosomes obtained from the stationary phase of growth in the presence of optimal concentrations of spermidine. 相似文献
74.
75.
The yeastSaccharomyces cerevisiae X2180-1A (wild) and its mutants X2180-1A-4 (mnn 1) and X2180-1A-5 (mnn 2) defective in mannan biosynthesis were used as enzyme sources to catalyzein vitro mannosyl transfer from GDP-[14C-U]-mannose to endogenous glycoproteins as well as to exogenous, low-molecular weight acceptors. While the enzyme preparation
from the wild strain exhibited all mannosyl transferase activities involved in mannan biosynthesis by catalyzing the synthesis
of characteristic mannoprotein, the enzyme frommnn 1 mutant failed to catalyze the synthesis of α(1→3) mannoside linkages both with endogenous as well as with exogenous acceptors.
The enzyme preparation from themnn 2 mutant catalyzed the formation of mannoprotein very similar to that obtained with the enzyme from the wild strain. The
most important difference was the formation of a higher number of unsubstituted mannosyl units in the α(1→ 6) linked mannan
backbone. The observed results support the hypothesis that in themnn 1 the mutation has altered the structural gene involved in biosynthesis of an α(1→3) mannosyl transferase catalyzing the addition
of α(1→3) linked mannosyl units to α(1→2) linked mannotrioses in the polysaccharide side chains and in the oligosaccharides
attached to serine and/or threonine in the protein part of mannan molecule. Themnn 2 mutant represents most probably a kind of regulatory mutation where the activity of an α(1→2) mannosyl transferase adding
the mannosyl units directly to α(1→6) linked backbone in the outer region of polysacoharide part of yeast mannan is repressedin vivo but becomes significantin vitro. 相似文献
76.
Effect of gibberellic Acid on crown gall tumor induction in aging primary pinto bean leaves 下载免费PDF全文
Gibberellic acid was tested for its effect on tumor induction by Agrobacterium tumefaciens in primary pinto bean (Phaseolus vulgaris) leaves in various stages of development. The hormone was found to promote tumor induction in partially aged leaves but did not effect tumor induction in very young leaves or in fully matured leaves. It is suggested that the natural loss of susceptibility to tumor induction in maturing pinto bean leaves is associated with a concomitant loss of endogenous gibberellins and/or a sensitivity to gibberellins. 相似文献
77.
78.
Exonuclease V from Saccharomyces cerevisiae. A 5'----3'-deoxyribonuclease that produces dinucleotides in a sequential fashion 总被引:3,自引:0,他引:3
A novel deoxyribonuclease, exonuclease V, has been purified approximately 30,000-fold from Saccharomyces cerevisiae. Exonuclease V is localized in the nucleus. The nuclease degrades single-stranded, but not double-stranded, DNA from the 5'-end. The products of exonuclease action are dinucleotides, except the 3'-terminal tri- and tetranucleotides which are not degraded. Studies with synthetic oligo- and polynucleotides with specified sequence elements showed that exonuclease V cleaves off dinucleotides as primary digestion products. Thus, the polymers (pT)9pA(pT)n and (pT)10pA(pT)n yielded pTpA and pApT as digestion products, respectively. Removal of the 5'-terminal phosphate from the DNA substrate results in reduced binding of the enzyme to the substrate. In addition, the initial hydrolytic cut by exonuclease V on the dephosphorylated substrate produces a mixture of dinucleoside monophosphates and trinucleoside diphosphates. The enzyme is processive in action. 相似文献
79.
T-cell intracellular antigen-1 (TIA-1) regulates developmental and stress-responsive pathways through distinct activities at the levels of alternative pre-mRNA splicing and mRNA translation. The TIA-1 polypeptide contains three RNA recognition motifs (RRMs). The central RRM2 and C-terminal RRM3 associate with cellular mRNAs. The N-terminal RRM1 enhances interactions of a C-terminal Q-rich domain of TIA-1 with the U1-C splicing factor, despite linear separation of the domains in the TIA-1 sequence. Given the expanded functional repertoire of the RRM family, it was unknown whether TIA-1 RRM1 contributes to RNA binding as well as documented protein interactions. To address this question, we used isothermal titration calorimetry and small-angle X-ray scattering to dissect the roles of the TIA-1 RRMs in RNA recognition. Notably, the fas RNA exhibited two binding sites with indistinguishable affinities for TIA-1. Analyses of TIA-1 variants established that RRM1 was dispensable for binding AU-rich fas sites, yet all three RRMs were required to bind a polyU RNA with high affinity. Small-angle X-ray scattering analyses demonstrated a "V" shape for a TIA-1 construct comprising the three RRMs and revealed that its dimensions became more compact in the RNA-bound state. The sequence-selective involvement of TIA-1 RRM1 in RNA recognition suggests a possible role for RNA sequences in regulating the distinct functions of TIA-1. Further implications for U1-C recruitment by the adjacent TIA-1 binding sites of the fas pre-mRNA and the bent TIA-1 shape, which organizes the N- and C-termini on the same side of the protein, are discussed. 相似文献
80.
Thyrotropin-releasing hormone-degrading ectoenzyme (TRH-DE) is a TRH-specific peptidase which catalyzes the inactivation of the peptidergic signal substance TRH. As indicated by sequence alignment, TRH-DE and the other members of the M1 family of aminopeptidases have a distinct set of conserved amino acid residues in common. By replacing amino acid residues that are putatively involved in catalysis, we could demonstrate that the enzymatic activities of the mutants E408D, E442D, E464Q, E464D, Y528F, H507R, and H507F are dramatically decreased, essentially due to the changes of V(max). The mutant enzymes E408Q and E442Q are inactive, whereas the specific enzymatic activity of the mutants R488Q, R488A, and Y554F are similar to that of the wild-type enzyme. These data strongly suggest that E408, E442, Y528, and H507 are involved in the catalytic process of TRH-DE while E464 presumably represents the third zinc-coordinating residue and may be equivalent to E166 in thermolysin. In contrast, amino acid residues R488 and Y554 seem not to be involved in the catalytic mechanism of TRH-DE. 相似文献